Microflora of bile aspirates in patients with acute cholecystitis With or without cholelithiasis: a tropical experience

Capoor, Malini R.; Nair, Deepthi; Rajni, .; Khanna, Geetika; Krishna, Suganeswari; Chintamani, M S; Aggarwal, Praveen

doi:10.1590/s1413-86702008000300012

Cited by 52 publications

(48 citation statements)

References 15 publications

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“…The results are less homogeneous in the Asiatic surveys from India (E. coli, Klebsiella spp., Citrobacter freundii, and Salmonella Typhi) [35] and Sri Lanka (E. coli, Pseudomonas spp., Streptococcus spp., and Klebsiella spp.) [36], with important differences when compared to our study.…”

Section: Discussionmentioning

confidence: 99%

Gallbladder microbiota variability in Colombian gallstones patients

Arteta¹,

Carvajal-Restrepo²,

Sánchez-Jiménez³

et al. 2017

J Infect Dev Ctries

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Introduction: Gallbladder stones are a very frequently occurring condition. Despite bile bactericidal activity, many bacteria have been detected inside the gallbladder, and gallstones facilitate their presence. Between 3% and 5% of the patients with Salmonella spp. infection develop the carrier stage, with the bacteria persisting inside the gallbladder, shedding bacteria in their feces without signs of infection. The aim of this study was to isolate bacteria from Colombian patients with gallstones, using standard culturing methods, and to identify Salmonella spp. carriers by molecular techniques. Methodology: A total of 149 patients (120 female and 29 male) diagnosed with gallstones who underwent cholecystectomy and who did not have symptoms of acute inflammation were included. Gallbladder tissue and bile were cultured and used for DNA extraction and Salmonella spp. hilA gene detection. Results: Of the 149 patients 28 (19%) had positive cultures. Twenty-one (75%) patients with positive cultures were from Medellin's metropolitan area. In this geographical location, the most frequent isolations were Pseudomonas spp. (38%), Klebsiella spp. (23%), and Proteus spp. (9%) in addition to unique cases of other bacteria. In Apartado, the isolates found were Enterobacter cloacae (50%), Raoultella terrigena (32%), and both Enterobacter cloacae and Raoultella terrigena were isolated in one (18%) male patient. Five (3.3%) of the 149 patients had positive polymerase chain reaction (PCR) results for the hilA gene of Salmonella spp., all of whom were female and residents of the Medellín metropolitan area. Conclusions: The gallbladder microbiota variability found could be related to geographical, ethnic, and environmental conditions.

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Section: Discussionmentioning

confidence: 99%

Gallbladder microbiota variability in Colombian gallstones patients

Arteta¹,

Carvajal-Restrepo²,

Sánchez-Jiménez³

et al. 2017

J Infect Dev Ctries

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“…(8) Gill et al in 2016 (1) has reported a slightly higher incidence of bacterbilia of about 30-35%. In a study by Capoor et al (2008), (9) most common organism was isolated was E. coli followed by Klebsiella pneumonia, Citrobacterfreundii and Salmonella. A number of other authors (Bhansali et al in 1985, (10) Cristina et al in 2003 (11) and Fazal et al in 2014) have reported E Coli to be the most common organism isolated from bile.…”

Section: Discussionmentioning

confidence: 99%

Bacteriological Profile of Bile and Antibiogram in Cholecystectomy Patients

Gupta¹

2017

jmscr

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“…or Vibrio cholerae) and other systemic infections (tuberculosis and syphilis). 3 Bacterial infection has been considered a primary factor, not only in the pathogenesis of brown stones, but also in the formation of black pigment stones. Although bile is usually sterile, bacteria isolated from intraoperative bile culture was 25%, 66%, 67% and 9% for gall bladder (GB) stones, common bile duct (CBD) stones, intrahepatic duct (IHD) stones and biliary malignancy, respectively.…”

Section: Introductionmentioning

confidence: 99%

Microflora of gall bladder bile in patients undergoing laparoscopic cholecystectomy

Prabhu¹,

Chandan²,

Sudarsan³

2018

Int Surg J

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Background: Gallstone disease is one of the most common problem affecting the digestive tract. The conditions that predispose to the development of gallstones are obesity, pregnancy, dietary factors, Crohn's disease, gastric surgery, terminal resection, hereditary spherocytosis, sickle cell disease, and thalassemia. The incidence is four times higher in women than in men with high prevalence among the age group of 30-40 years. Bacteria are commonly found in inflamed gallbladder and in patients with cholelithiasis, whereas evidence suggests that normal bile is sterile. So, exploring and identifying the microflora of gallbladder bile helps to choose appropriate antibiotics. Methods: Minimum of 50 Patients admitted under General Surgery, who are scheduled to undergo laparoscopic cholecystectomy. Five ml of bile will be aspirated from the gall bladder during laparoscopic cholecystectomy. The samples will be transferred onto a plain sterile container and then transferred at room temperature in an hour to the laboratory for culture. Results: In the present study 50 patients were evaluated for culture and gall bladder bile. Out of which maximum number of patients were clustered between 31-40 years. In the present study out of 50 patients, 34 (68%) were females and 16 (32%) were males. In the present study We found total of 33 (66%) yellow stones, 13 (26%) were black in colour and 4(8%) were brown stones. In this study bile culture was showing growth of the following organisms. The commonest organism was E. coli (64%) followed by Klebsiella (25%) and Enterococcus (11%). Conclusions: Bactibilia has long been known to be associated with biliary tract diseases and culturable bacteria in bile can represent a state of asymptomatic bactibilia which can disseminate after any intervention causing infective complication. Exploring the microflora of gall bladder bile important role in choosing the appropriate antibiotic to prevent complications.

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Microflora of bile aspirates in patients with acute cholecystitis With or without cholelithiasis: a tropical experience

Cited by 52 publications

References 15 publications

Gallbladder microbiota variability in Colombian gallstones patients

Gallbladder microbiota variability in Colombian gallstones patients

Bacteriological Profile of Bile and Antibiogram in Cholecystectomy Patients

Microflora of gall bladder bile in patients undergoing laparoscopic cholecystectomy

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