Microflora in the Hemolymph of Japanese Coastal Crustacea

Ueda, Ryutaro; Sugita, Haruo; Deguchi, Yoshiaki

doi:10.2331/suisan.59.1637

Cited by 6 publications

(6 citation statements)

References 4 publications

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“…Although a number of studies report high numbers of bacteria within the body cavities of apparently healthy crustaceans , Sizemore et al 1975, Ueda et al 1993, this study has shown that the existence of bacteria in the body cavity may have apparent detrimental effects on the animal. Vogan et al (1999) showed that shell-diseased male Cancer pagurus displayed a significantly higher percentage of limb loss than their disease-free counterparts.…”

Section: Discussionmentioning

confidence: 60%

A histological study of shell disease syndrome in the edible crab Cancer pagurus

Vogan¹,

Costa-Ramos²,

Rowley³

2001

Dis. Aquat. Org.

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Shell disease syndrome is characterised by the external manifestation of black spot lesions in the exoskeletons of crustaceans. In the present study, gills, hepatopancreas and hearts from healthy (< 0.05% black spot coverage) and diseased (5 to 15% coverage) edible crabs, Cancer pagurus, were examined histologically to determine whether this disease can cause internal damage to such crabs. There was clear evidence of cuticular damage in the gills of diseased crabs leading to the formation of haemocyte plugs termed nodules. Nephrocytes found within the branchial septa of the gills showed an increase in the accumulation of dark material in their vacuoles in response to disease. In the hepatopancreas, various stages of tubular degradation were apparent that correlated with the severity of external disease. Similarly, there was a positive correlation between the number of viable bacteria in the haemolymph and the degree of shell disease severity. Approximately 21% of the haemolymph-isolated bacteria displayed chitinolytic activity. Overall, these findings suggest that shell disease syndrome should not be considered as a disease of the cuticle alone. Furthermore, it shows that in wild populations of crabs shell perforations may lead to limited septicaemia potentially resulting in damage of internal tissues. Whether such natural infections lead to significant fatalities in crabs is still uncertain.

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Section: Discussionmentioning

confidence: 60%

A histological study of shell disease syndrome in the edible crab Cancer pagurus

Vogan¹,

Costa-Ramos²,

Rowley³

2001

Dis. Aquat. Org.

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“…However, the presence of bacteria in the haemolymph of Crustacea, previously interpreted as a disease indicator (Lightner 1975;Lightner and Lewis 1975), is currently viewed more as a natural process (Gomez-Gil et al 1998). Bacteria have been isolated from the haemolymph of apparently healthy Pacific white shrimp (Penaeus vannamei;Gomez-Gil et al 1998), American lobster (Homarus americanus; Cornick and Stewart 1966;Bartlett et al 2008), blue crab (Callinectes sapidus; Haskell et al 1975;Tubiash et al 1975;Davis and Sizemore 1982;Welsh and Sizemore 1985), red swamp crawfish (Procambarus clarkii; Scott and Thune 1986), diverse crab species (Plagusia dentipes, Schizophrys aspera, Atergatis spp., and Aniculus aniculus; Ueda et al 1993), and freshwater prawns (Macrobrachium rosenbergii; Brady and Lasso-de-la-Vega 1992). Bacterial density often does not exceed 10 3)4 CFU ml )1 of haemolymph (Tubiash et al 1975;Welsh and Sizemore 1985;Ueda et al 1993;Gomez-Gil et al 1998) in 88AE3% of the analysed organisms (Bartlett et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…1975; Tubiash et al. 1975; Davis and Sizemore 1982; Welsh and Sizemore 1985), red swamp crawfish ( Procambarus clarkii ; Scott and Thune 1986), diverse crab species ( Plagusia dentipes , Schizophrys aspera , Atergatis spp., and Aniculus aniculus ; Ueda et al. 1993), and freshwater prawns ( Macrobrachium rosenbergii ; Brady and Lasso‐de‐la‐Vega 1992).…”

Section: Introductionmentioning

confidence: 99%

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Diversity of vibrios in the haemolymph of the spider crab Maja brachydactyla

Gómez‐Gil

Roque

Lacuesta

et al. 2010

Journal of Applied Microbiology

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Aims: The aim of this study was to characterise and identify vibrios isolated from the haemolymph of apparently healthy adult spider crabs (Maja brachydactyla) wild‐caught in the Spanish localities of Galician coast and in the Canary Islands and also from captive animals held at IRTA’s facilities in the Ebro Delta of Catalonia, north‐west Spanish Mediterranean coast. Methods and Results: A total of 277 bacterial isolates were obtained, and of these, 171 were characterised with rep‐PCR, resulting electrophoretic bands were analysed and clusters formed. Identification of representative strains of each cluster was made by sequencing the 16S rRNA. Samples from animals caught in Galicia and captive at IRTA (around 15–18°C) rendered mostly species belonging to the Splendidus clade (72·2 and 76·6% respectively), commonly found in cold waters (below 20°C). Higher species diversity was found in the haemolymph of the captive animals. In the warmer Canary Islands waters (around 21°C), the diversity of vibrios is dominated by three clades, Harveyi (Vibrio core group, 39·3%), Orientalis (23·2%) and Splendidus (21·4%) with a species diversity that equals that of the colder captive animals. Conclusions: Differences in the vibrios populations were found in the haemolymph extracted from animals collected from the three localities. Potential new species were found, and their description is under way. Significance and Impact of Study: As with other invertebrates, spider crabs also contain a diverse population of vibrios. These findings should help researchers to diagnose when a crab is infected.

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“…This may involve the fact that population densities of Gram-positive bacteria are much lower than Gram-nega tive bacteria in the intestine and hemolymph of Japanese crustaceans and their environments. [11][12][13] It is likely that this phenomenon is attributed to the structure of cell sur face of bacteria to some extent.…”

mentioning

confidence: 99%

Determination of the Naturally Occurring Bactericidal Activity of Sera from Japanese Coastal Crustaceans

1996

Self Cite

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Many marine crustaceans possess humoral and cellular defense mechanisms such as agglutinin, bactericidin and phagocytes, which cooperatively act to protect crustaceans from bacterial pathogens.1-8) Of these defense factors, bac tericidin is assumed to be more important for the protec tion against invading bacteria because this factor directly kills bacterial cells. The bactericidal activity of crustacean

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Microflora in the Hemolymph of Japanese Coastal Crustacea

Cited by 6 publications

References 4 publications

A histological study of shell disease syndrome in the edible crab Cancer pagurus

A histological study of shell disease syndrome in the edible crab Cancer pagurus

Diversity of vibrios in the haemolymph of the spider crab Maja brachydactyla

Determination of the Naturally Occurring Bactericidal Activity of Sera from Japanese Coastal Crustaceans

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