Microfiltration of protein mixtures and the effects of yeast on membrane fouling

Güell, Carme; Czekaj, Piotr; Davis, Robert H.

doi:10.1016/s0376-7388(98)00305-6

Cited by 97 publications

(46 citation statements)

References 17 publications

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“…The observed gradual decrease in membrane permeability results from chemical bonding of native protein molecules to the aggregates adsorbed to the membrane surface. The bonding is usually a disulfide covalent bond, although it is assumed that aggregate formation is also to a certain degree determined by weak van der Waals force, electrostatic and hydrophobic interactions, as well as hydrogen bonds (Marshall et al 1997;Güell et al 1999). The results seem to be consistent with the literature data, especially in the light of the fact that the microfiltration membrane used allowed a reduction in TSS of over 93 % (Fig.…”

Section: Integrated Treatment Of Process Wastewater By Mf Uf and MDsupporting

confidence: 88%

Protein and water recovery from poultry processing wastewater integrating microfiltration, ultrafiltration and vacuum membrane distillation

Białas

Stangierski

Konieczny

2014

Int. J. Environ. Sci. Technol.

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A novel approach for the recovery of the proteins contained in the effluent formed during production of surimi-like material from mechanically recovered poultry meat was proposed, using an integrated membrane process comprising microfiltration, ultrafiltration and vacuum membrane distillation. Additionally, the possibility of using the integrated membrane process to achieve highquality water for reuse was also investigated. Using crossflow membrane techniques, it was found that the total recovery of soluble proteins as well as the average degree of concentration amounted to 84 % and 9.3, respectively. Moreover, using this process, the total recovery of water was 70 % and the percentage reduction in COD, TOEM and TSS in the permeate obtained using vacuum membrane distillation exceeded 99 %. As a result, the water recovered by the integrated membrane process could be reused in the manufacture of surimi-like material from mechanically recovered poultry meat.

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Section: Integrated Treatment Of Process Wastewater By Mf Uf and MDsupporting

confidence: 88%

Protein and water recovery from poultry processing wastewater integrating microfiltration, ultrafiltration and vacuum membrane distillation

Białas

Stangierski

Konieczny

2014

Int. J. Environ. Sci. Technol.

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“…They found that the transition to a slower plugging rate occurs during filtration at constant rate. Guell et al have studied the effect of yeast cells on membrane fouling for different protein mixtures in dead-end filtration [17]. They observed that the yeast cake on top of the primary membrane acts as a secondary membrane and retains protein aggregates, thereby reducing protein fouling of the primary membrane.…”

Section: Introductionmentioning

confidence: 99%

Modeling of Filtration Processes—Microfiltration and Depth Filtration for Harvest of a Therapeutic Protein Expressed in Pichia pastoris at Constant Pressure

2014

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Filtration steps are ubiquitous in biotech processes due to the simplicity of operation, ease of scalability and the myriad of operations that they can be used for. Microfiltration, depth filtration, ultrafiltration and diafiltration are some of the most commonly used biotech unit operations. For clean feed streams, when fouling is minimal, scaling of these unit operations is performed linearly based on the filter area per unit volume of feed stream. However, for cases when considerable fouling occurs, such as the case of harvesting a therapeutic product expressed in Pichia pastoris, linear scaling may not be possible and current industrial practices involve use of 20-30% excess filter area over and above the calculated filter area to account for the uncertainty in scaling. In view of the fact that filters used for harvest are likely to have a very limited lifetime, this oversizing of the filters can add considerable cost of goods for the manufacturer. Modeling offers a way out of this conundrum. In this paper, we examine feasibility of using the various proposed models for filtration of a therapeutic product expressed in Pichia pastoris at constant pressure. It is observed that none of the individual models yield a satisfactory fit of the data, thus indicating that more than one fouling mechanism is at work. Filters with smaller pores were found to undergo fouling via complete pore blocking followed by cake filtration. On the other hand, filters with larger pores were found to undergo fouling via intermediate pore blocking followed by cake filtration. The proposed approach can be used for more accurate sizing of microfilters and depth filters.

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“…A popular model protein-cell system is the mixture of washed yeast cells and bovine serum albumin (BSA) (Arora and Davis, 1994;Davis, 1999, 2001;Ye and Chen, 2005). The more complex scenario of washed yeast cells and a mixture of proteins has also been studied (Guell et al, 1999). In all cases, a cake of yeast cells was found to form on the membrane surface, and under conditions where the cake acts as a filter aid, an enhancement in the flux and protein transmission above that for filtration of the protein alone maybe achieved (Guell et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…The more complex scenario of washed yeast cells and a mixture of proteins has also been studied (Guell et al, 1999). In all cases, a cake of yeast cells was found to form on the membrane surface, and under conditions where the cake acts as a filter aid, an enhancement in the flux and protein transmission above that for filtration of the protein alone maybe achieved (Guell et al, 1999). A cake may be considered to be a filter aid if the cake captures foulant species, stopping these species reaching the membrane, and that the consequent reduction in fouling at the membrane outweighs any increase in specific resistance due to the blocking of the interstices in the cake by captured species (Hughes and Field, 2006a).…”

Section: Introductionmentioning

confidence: 99%

Membrane fouling by cell‐protein mixtures: In situ characterisation using multi‐photon microscopy

Hughes

Cui

Field

et al. 2006

Biotech & Bioengineering

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Fouling of the membrane by cell and protein mixtures can result in severe flux declines, leading to the eventual need to clean or replace the membrane. In this study multi-photon microscopy, a fluorescence-based technique is used to 3-D image in situ the fouling of microfiltration membranes by suspensions containing combinations of washed yeast, bovine serum albumin (BSA) and ovalbumin. Appropriate fluorescent labelling allows the three foulant species to be clearly identified. Images correlate well with filtration data and clearly show the cake of yeast cells capturing protein aggregates. The proteins exhibited very different filtration behaviour. When filtering washed yeast together with ovalbumin and/or a 50:50 mixture by mass of BSA and ovalbumin, the ovalbumin fouling dominates the system. Capture of aggregates by the cake did not reduce fouling of the membrane by the protein and increased the resistance of the cake. For mixtures of BSA and washed yeast, the presence of a cake of yeast cells did reduce fouling of the membrane by the protein, however, the extra resistance due to the cake resulted in a flux lower than that when filtering BSA alone.

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Microfiltration of protein mixtures and the effects of yeast on membrane fouling

Cited by 97 publications

References 17 publications

Protein and water recovery from poultry processing wastewater integrating microfiltration, ultrafiltration and vacuum membrane distillation

Protein and water recovery from poultry processing wastewater integrating microfiltration, ultrafiltration and vacuum membrane distillation

Modeling of Filtration Processes—Microfiltration and Depth Filtration for Harvest of a Therapeutic Protein Expressed in Pichia pastoris at Constant Pressure

Membrane fouling by cell‐protein mixtures: In situ characterisation using multi‐photon microscopy

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