Microfabrication of a tunable collagen/alginate-chitosan hydrogel membrane for controlling cell–cell interactions

Song, Yukun; Zhang, Demeng; Lv, Yan; Guo, Xin; Lou, Ruyun; Wang, Shujun; Wang, Xiuli; Wu, Yu; Ma, Xiaojun

doi:10.1016/j.carbpol.2016.07.058

Cited by 17 publications

(7 citation statements)

References 48 publications

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“…Chitin and CS belong to the family of glycosaminoglycans and constitute a series of the linear copolymers of N-acetyl-D-glucosamine and D-glucosamine with β (1 → 4) glycosidic linkages [ 8 ]. They have been widely used in cells encapsulation, cell culture [ 9 ], bone reconstruction and pharmaceutical fields for their bioactivity, biocompatibility, biodegradability and interesting physicochemical properties [ 10 ]. Mizuno et al [ 11 ] reported that CS is able to incorporate basic fibroblast growth factors to accelerate the rate of wound healing.…”

Section: Introductionmentioning

confidence: 99%

Construction of physical crosslink-based chitosan/liquid crystal composite hydrogel and evaluation on their cytocompatibility

Du¹,

Yang

et al. 2016

Regen Biomater

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In order to provide a novel biomimetic composite substrate for tissue engineering and explore the interaction between cells and this type of material, we developed chitosan/liquid crystal (CS/LC) composite hydrogel with embedded LC phases by composing of cholesterol hydroxypropyl cellulose ester liquid crystalline material and CS. The micromorphology of CS/LC composite hydrogels exhibited ‘islands-sea’ phase separation structures similar to the ‘fluid mosaic model’ of biomembrane. In vitro cell compatibility study suggested that 3T3 is fibroblasts exhibited better initial cell adhesions and higher proliferation rates on the composite hydrogel than on the polystyrene control plate and the pure LC membrane. This novel CS/LC composite hydrogel provides more favorable interface for cell growth and proliferation and may serve as potentially active substrate for engineering interfaces to live cells.

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Section: Introductionmentioning

confidence: 99%

Construction of physical crosslink-based chitosan/liquid crystal composite hydrogel and evaluation on their cytocompatibility

Du¹,

Yang

et al. 2016

Regen Biomater

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“…The hydrogel was prepared according to the method previously described with some modifications ( Song et al, 2016 ). Briefiy, sodium alginates (High-G/M and Mid-G/M) were dissolved in 0.9% (w/v) NaCl solution to a final concentration of 60 mg/mL.…”

Section: Methodsmentioning

confidence: 99%

Effect of the uronic acid composition of alginate in alginate/collagen hybrid hydrogel on chondrocyte behavior

Zheng

Xue²,

Cao³

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: Developing a culture system that can effectively maintain chondrocyte phenotype and functionalization is a promising strategy for cartilage repair.Methods: An alginate/collagen (ALG/COL) hybrid hydrogel using different guluronate/mannuronate acid ratio (G/M ratio) of alginates (a G/M ratio of 64/36 and a G/M ratio of 34/66) with collagen was developed. The effects of G/M ratios on the properties of hydrogels and their effects on the chondrocytes behaviors were evaluated.Results: The results showed that the mechanical stiffness of the hydrogel was significantly affected by the G/M ratios of alginate. Chondrocytes cultured on Mid-G/M hydrogels exhibited better viability and phenotype preservation. Moreover, RT-qPCR analysis showed that the expression of cartilage-specific genes, including SOX9, COL2, and aggrecan was increased while the expression of RAC and ROCK1 was decreased in chondrocytes cultured on Mid-G/M hydrogels.Conclusion: These findings demonstrated that Mid-G/M hydrogels provided suitable matrix conditions for cultivating chondrocytes and may be useful in cartilage tissue engineering. More importantly, the results indicated the importance of taking alginate G/M ratios into account when designing alginate-based composite materials for cartilage tissue engineering.

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“…* Seaweed, * Annual plants, * Seed fibres, * Bacteria * Algae * * Green * Sustainable * Inexpensive * Hydrophilic * Most abundant resource * Biodegradable * Heavy metal removal * Packaging * Dye removal * Water purification * Biomedical * * Costless * Plentiful * Non-toxic * Eco-friendly * Degradable * Innocuous, * Hydrophilicity, * Biocompatibility * * Separation of oil and water emulsion * Used as an additive for improvement in properties, * Drug delivery systems * Removal of Organic Dyes * * Skin, * Vasculature organs, * Ligaments, * Tendon * Cornea * Cartilage, * Intervertebral discs, * Vitreous body * Reticulate, granulation tissue * Forms basal lamina * Cell surfaces, hair * Placenta * Good homeostatic capacity * Structural viability * Chemotactic action on regenerative cells * Low immunogenicity * Good dimensional stability * Biodegradable * Selective permeance * Good recycling * Separation of Emulsions * * Nails * Feathers * Wool * Hair * Horns * Claws * Hooves * The outer skin of vertebrates * Abundant, * Chemically Stable, * Biocompatibility * Biodegradability * Fibrous pore size * High porosity, * High permeance, * Adsorption, * * Guided tissue regeneration, * Separation of heavy metals (Cu, Ni, Co), * Removes dyes from water, * Cosmetics and food packing * * Sea crustaceans * Antibacterial * Antioxidant * Wound dressing applications[38,42,44,45,[54][55][56] …”

mentioning

confidence: 99%

Green and Non‐Conventional Materials for Membrane Synthesis: A Review

et al. 2022

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Membrane separation has been an upgrade in our quest for eco-friendly processes because of its minimal energy usage and lesser waste generation. However, the most widely used membranes are polymeric membranes. Most of these membranes are non-biodegradable and are replaced after deterioration/fouling and then discarded. Dumping without treatment on a large scale is a significant environmental problem. Green polymers only make up a limited percentage of all the new materials that are reported every year. We require more research in developing renewable, inexpensive membranes from waste materials to ensure progress with the least toll on the environment. Several reported green methods can partially achieve green synthesis. This ensures the least use of nonbiodegradable polymers in membranes. This review will emphasize the exploration of more natural materials given the target is to fabricate membranes in a way, where both raw material and membrane preparation are green.

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Microfabrication of a tunable collagen/alginate-chitosan hydrogel membrane for controlling cell–cell interactions

Cited by 17 publications

References 48 publications

Construction of physical crosslink-based chitosan/liquid crystal composite hydrogel and evaluation on their cytocompatibility

Construction of physical crosslink-based chitosan/liquid crystal composite hydrogel and evaluation on their cytocompatibility

Effect of the uronic acid composition of alginate in alginate/collagen hybrid hydrogel on chondrocyte behavior

Green and Non‐Conventional Materials for Membrane Synthesis: A Review

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