Microfabrication and Characterization of Diaphorase-Patterned Surfaces by Scanning Electrochemical Microscopy

Abstract

Scanning electrochemical microscopy (SECM) was used to microfabricate and quantify diaphorase-pattemed glass surfaces. Deactivated circular and linear micropattems were produced at diaphorase-immobilized substrates by a localized surface reaction. The oxidation of Br~and Clã t a microelectrode generated a reactive species which deactivated the localized enzyme molecules at the substrate. The diaphorase-pattemed surfaces were characterized by SECM on the basis of detection of catalytic current of ferrocenylmethanol coupled with oxidation of NADH. The concentration of the immobilized diaphorase

Intrinsic Electrochemical Characteristics in the Individual Needle-like LiCoO₂ Crystals Synthesized by Flux Growth

“…For the single particle measurements, a lab-made Pt microprobe was used to make electrical connection with a needlelike LiCoO 2 particle; details of the fabrication procedure have been described elsewhere. 31 Before the measurement, the Pt microprobe was subjected to cyclic voltammetry in an aqueous 0.5 M H 2 SO 4 solution for the cleaning. The single particle measurements were carried out in a super-dry room at room temperature (298 K).…”

Scanning electrochemical microscopy: beyond the solid/liquid interface

“…Matsue and co-workers [66] fabricated and characterised diaphorase-patterned surfaces at the micrometre level. Additional work by Shiku et al [67] found that by coupling SECM with the enzyme-linked immunosorbent assay (ELISA) technique, detection levels as low as 10 4 molecules in a single 20 mm radius spot could be obtained for a carcinoembryonic antigen.…”

Parallel Imaging and Template‐Free Patterning of Self‐Assembled Monolayers with Soft Linear Microelectrode Arrays

“…[1][2][3][4][5][6] The electrolytic generation of a reactive compound at the microelectrode (i.e. the SECM probe) allows the chemically well-defined transformation of various materials and layers that can be quantitatively controlled by adjusting the working distance, electrolysis current, and electrolysis time.…”

“…Br 2 and HOBr act as oxidants and have been used for semiconductor etching, [1,8] polyaniline deposition, [3] and the deactivation of enzyme layers. [2] Other probe-generated reagents include metal ions for metal deposition, [9,10] catalytically active metal ions, [11] and aryl diazonium salts. [6,12] The pH shift associated with nitrite oxidation was applied in the local etching of a thin Ni coating on a gold substrate in a chemical lens.…”