Microevolution of a Mycobacteroides abscessus subsp. bolletii strain in a clinical persistent infection

Santos, Andrea; Pinto, Miguel; Carneiro, Sofia; Silva, Sónia; Rodrigues, Irene Pimenta; Munhá, João; Gomes, João Paulo; Macedo, Rita

doi:10.1016/j.meegid.2023.105437

Cited by 1 publication

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“…Moreover, isolates from the same patient were closely linked (maximum of 16 ADs) in four distinct genetic clusters (Figure 3), while also being genetically distant from other isolates (minimum of 43 ADs observed between PTNTM_0014 and PTNMT_0024). Of note, the genetic cluster composed of isolates PTNTM_0001 up to PTNTM_0005 corresponds to an in-depth studied persistent infection by M. bolletii observed in a single patient from 2015 to 2021 [38]. Minimum spanning tree was constructed using Grapetree [39] based on the allelic matrix of 29 isolates and 3458 loci generated with the MABC wgMLST schema, with a site inclusion tolerance of 1% missing data.…”

Section: Mabc Phylogenetic Analysismentioning

confidence: 99%

Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal

Carneiro,

Pinto,

Silva

et al. 2023

IJMS

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The Mycobacterium abscessus complex (MABC) is an emerging, difficult to treat, multidrug-resistant nontuberculous mycobacteria responsible for a wide spectrum of infections and associated with an increasing number of cases worldwide. Dominant circulating clones (DCCs) of MABC have been genetically identified as groups of strains associated with higher prevalence, higher levels of antimicrobial resistance, and worse clinical outcomes. To date, little is known about the genomic characteristics of MABC species circulating in Portugal. Here, we examined the genetic diversity and antimicrobial resistance profiles of 30 MABC strains isolated between 2014 and 2022 in Portugal. The genetic diversity of circulating MABC strains was assessed through a gene-by-gene approach (wgMLST), allowing their subspecies differentiation and the classification of isolates into DCCs. Antimicrobial resistance profiles were defined using phenotypic, molecular, and genomic approaches. The majority of isolates were resistant to at least two antimicrobials, although a poor correlation between phenotype and genotype data was observed. Portuguese genomes were highly diverse, and data suggest the existence of MABC lineages with potential international circulation or cross-border transmission. This study highlights the genetic diversity and antimicrobial resistance profile of circulating MABC isolates in Portugal while representing the first step towards the implementation of a genomic-based surveillance system for MABC at the Portuguese NIH.

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Section: Mabc Phylogenetic Analysismentioning

confidence: 99%