Microenvironmental reprogramming of human dermal papilla cells for hair follicle tissue engineering

Liu, Zhen; Huang, Junfei; Kang, Deni; Zhou, Yi; Du, Lijuan; Qu, Qian; Wang, Jin; Wen, Lihong; Fu, Danlan; Hu, Zhiqi; Miao, Yong

doi:10.1016/j.actbio.2022.11.004

Cited by 15 publications

(5 citation statements)

References 69 publications

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“…43 To reproduce hair follicles, which are complex organs, various studies have been conducted to produce spheroids using a combination of various cells or using transfected cells. [18][19][20][21] However, there are no reports of spheroids produced by combining various cells with transfected cells. DPCs play a role in controlling hair growth and fluff development, and KCs form the epidermis of the hair follicle.…”

Section: Discussionmentioning

confidence: 99%

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Optimization of hair follicle spheroids for hair-on-a-chip

Jeong,

Nam,

Sung

2024

Biomater. Sci.

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Section: Discussionmentioning

confidence: 99%

“…As a result, several specific essential genes were upregulated: ALP, NCAM2, SOX2, and β-catenin. 21 The development of hair-on-a-chip containing organoids will be useful for scalp drug development and cosmetic testing. It is also known that skin microbes are involved in the autoimmune response to alopecia areata.…”

Section: Introductionmentioning

confidence: 99%

Optimization of hair follicle spheroids for hair-on-a-chip

Jeong,

Nam,

Sung

2024

Biomater. Sci.

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“…Extensive research has since gone into improving the hair-inducing properties of cultured DP spheroids by culturing them in keratinocyte-conditioned media [76] or supplementing culture media with extracellular matrices (ECM). These include natural additives such as Matrigel [77] and synthetic ECM such as silk-gelatin [78], self-assembling peptide scaffold [79], and fibrin-microgel derived from blood plasma [80]. These strategies could enhance BMP and WNT/β-catenin signaling [78], improve cellular functions such as proliferation and migration [77,78], induce a secretome profile that resembles that of anagen DP [76], and support the cellular organization and viability of DP cells by further promoting their production of other ECMs such as collagen IV and versican [79,80].…”

Section: Dp Spheroidsmentioning

confidence: 99%

Modelling Human Hair Follicles—Lessons from Animal Models and Beyond

Tan,

Lim,

Lay

2024

Biology

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The hair follicle is a specialized appendage of the skin that is critical for multiple functions, including thermoregulation, immune surveillance, and sebum production. Mammals are born with a fixed number of hair follicles that develop embryonically. Postnatally, these hair follicles undergo regenerative cycles of regression and growth that recapitulate many of the embryonic signaling pathways. Furthermore, hair cycles have a direct impact on skin regeneration in homeostasis, cutaneous wound healing, and disease conditions such as alopecia. Here, we review the current knowledge of hair follicle formation during embryonic development and the post-natal hair cycle, with an emphasis on the molecular signaling pathways underlying these processes. We then discuss efforts to capitalize on the field’s understanding of in vivo mechanisms to bioengineer hair follicles or hair-bearing skin in vitro and how such models may be further improved to develop strategies for hair regeneration.

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“…Compared with biomaterials such as Matrigel [73,74], collagen [75], silk sericin [76] and peptides [11], GelMA is easier to prepare and can achieve thermodynamic compatibility with HAD, but lacks specific ligands or growth factors for stem cell activities and tissue regeneration, especially for HF regeneration. To ameliorate this problem, it was proposed to encapsulate FGF-7 with MSCs within the GelMA core in G/HAD microspheres to simulate the signal exchange from the HF mesenchyme to the epidermis (figure 4(D)).…”

Section: In Vivo and In Vivo Fgf-7 Release From The Microfluidic Micr...mentioning

confidence: 99%

One-step generation of core–shell biomimetic microspheres encapsulating double-layer cells using microfluidics for hair regeneration

Huang

et al. 2023

Biofabrication

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Tissue engineering of hair follicles (HFs) has enormous potential in the treatment of hair loss. HF morphogenesis is triggered by reciprocal interactions between HF germ epithelial and mesenchymal layers. Here, a microfluidic-assisted technology is developed for the preparation of double aqueous microdroplets that entrap double-layer cells and growth factors to ultimately be used for hair regeneration. Mouse mesenchymal cells (MSCs) and epidermal cells (EPCs) are encapsulated in gelatin methacrylate (GelMA) cores and photo-curable catechol-grafted hyaluronic acid (HAD) shells to fabricate GelMA-MSC/HAD-EPC (G/HAD) microspheres. The findings show that the G/HAD microspheres exhibit ultrafast gelation, aqueous phase separation, superior biocompatibility, and favorable wet adhesion properties. G/HAD microspheres can also support cell proliferation and sustain growth factor release. These composite cell microspheres are capable of efficient HF generation upon transplantation into the dorsal dermis of nude mice. This finding facilitates the large-scale preparation of approximately 80 double-layer cell spheres per min. This simple double-layer cell sphere preparation approach is a promising strategy for improving current hairregenerative medicine techniques and can potentially be applied along with other organoid techniques for extended applications.

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Microenvironmental reprogramming of human dermal papilla cells for hair follicle tissue engineering

Cited by 15 publications

References 69 publications

Optimization of hair follicle spheroids for hair-on-a-chip

Optimization of hair follicle spheroids for hair-on-a-chip

Modelling Human Hair Follicles—Lessons from Animal Models and Beyond

One-step generation of core–shell biomimetic microspheres encapsulating double-layer cells using microfluidics for hair regeneration

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