Microenvironment of Cysteine 242 in Type-1 Ribosome-Inactivating Protein from Iris

Hao, Qiang; Damme, Els J.M. Van; Barre, Annick; Sillen, Alain; Rougé, Pierre; Engelborghs, Yves; Peumans, Willy J.

doi:10.1006/bbrc.2000.3338

Cited by 6 publications

(3 citation statements)

References 35 publications

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“…Whether these transgenic tomato lines may show resistance to other fungal pathogens should be explored. Previously, Hao et al (2000) have reported that I-RIP is one of the few type-1 RIPs containing a cysteine residue, a single residue at position 242, in their primary sequences, although the cysteine residue is not critical for the RNA N-glycosidase activity of I-RIP. However, I-RIP shows selective binding to conspecific DNA fragments from the plant genome and some of which lower the RNA N-glycosidase activity of I-RIP (Hao et al 2001a).…”

Section: Discussionmentioning

confidence: 99%

Enhanced resistance to early blight in transgenic tomato lines expressing heterologous plant defense genes

Schaefer

Gašić

Cammue

et al. 2005

Planta

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Genes coding for an iris ribosomal-inactivating protein (I-RIP), a maize beta-glucanase (M-GLU), and a Mirabilis jalapa antimicrobial peptide (Mj-AMP1) were separately introduced into tomato (Lycopersicon esculentum cv. Sweet Chelsea) cotyledons via Agrobacterium tumefaciens-mediated transformation. Transgenic lines carrying each of the transgenes were confirmed for integration into the tomato genome using Southern blot hybridization. Transcription of I-RIP, M-GLU, and Mj-AMP1 genes in various transgenic lines was determined using Northern blot analysis. Plants of selected transgenic lines were inoculated with a 2-3x10(4) conidial spores/ml suspension of the fungal pathogen Alternaria solani, the causal agent of tomato early blight. Compared to control (non-transformed) plants, two transgenic lines carrying either a M-GLU or Mj-AMP1 showed enhanced resistance to early blight disease. None of the four lines carrying the I-RIP transgene showed increased resistance to early blight.

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Section: Discussionmentioning

confidence: 99%

Enhanced resistance to early blight in transgenic tomato lines expressing heterologous plant defense genes

Schaefer

Gašić

Cammue

et al. 2005

Planta

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“…Based on homology analysis of amino acid sequences among RA39 and other RIPs, and enzyme activity assay, we conclude that RA39 is a novel type I RIP. RIPs are a widely distributed group of plant proteins and have been identified in seeds, roots, leaves and bulbs of many monocots (except rice, until now) and dicots (Bass et al 1992;Vivanco et al 1999;Hao et al 2000;Krawetz and Boston 2000;Kwon et al 2000;Song et al 2000;Van Damme et al 2000). The discovery of RA39 demonstrates that RIPs also occur in the anthers of higher plants.…”

Section: Discussionmentioning

confidence: 99%

The rice tapetum-specific gene RA39 encodes a type I ribosome-inactivating protein

Ding

Wang

2002

Sexual Plant Reproduction

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A tapetum-specific cDNA encoded by a rice gene, RA39, was isolated by cDNA subtractive hybridization, differential screening and rapid amplification of cDNA ends. RA39 is a single-copy gene in the rice genome. mRNA in situ hybridization indicates that this gene is a tapetum-specific gene, and highly expressed in the tapetal cells at the meiosis and tetrad stages. The RA39 cDNA is 1,013 bp in length with an open reading frame encoding 298 amino acid residues. This cDNA sequence does not show significant homology to any known sequences in GenBank databases, but its deduced amino acid sequence (RA39) has between 19 and 34% sequence identity to ribosome-inactivating proteins (RIPs). Optimal alignment reveals that the five amino acid residues constituting the active site of the ricin A-chain (Tyr 80 , Tyr 123 , Glu 177 , Arg 180 and Trp 211 ), which are invariant among all RIPs published to date, are conserved in RA39. Recombinant RA39 protein expressed in Escherichia coli was purified to homogeneity. The purified protein exhibits the RNA N-glycosidase activity of RIPs. This demonstrates that RIPs occur in the reproductive organs of rice. The possible function of RA39 in anther development is discussed.

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“…Serum was collected by centrifugation and the antibodies purified by affinity chromatography on a Sepharose 4B column with immobilized IRIP. The monospecificity of the antiserum was checked by Western-blot analysis as described in [19].…”

Section: Methodsmentioning

confidence: 99%

Type-1 ribosome-inactivating protein from iris (Iris hollandica var. Professor Blaauw) binds specific genomic DNA fragments

Hao

Peumans

Damme

2001

Biochemical Journal

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The capacity of IRIP, a type-1 ribosome-inactivating protein (RIP) isolated from the bulbs of Iris hollandica, to bind specific DNA sequences from a mixture of approx. 200 bp (average length) fragments of total genomic DNA from Iris genome was studied. Fragments that were preferentially bound by IRIP were enriched by several cycles of affinity binding and PCR, and were cloned and sequenced. The selected DNA fragments do not share conserved sequences, indicating that IRIP does not bind DNA fragments in a strictly sequence-specific manner. According to sequence analysis, most IRIP-bound fragments contain one or more possible free energy-stable hairpin structure(s) in their secondary structure, which may be the basis for recognition

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Microenvironment of Cysteine 242 in Type-1 Ribosome-Inactivating Protein from Iris

Cited by 6 publications

References 35 publications

Enhanced resistance to early blight in transgenic tomato lines expressing heterologous plant defense genes

Enhanced resistance to early blight in transgenic tomato lines expressing heterologous plant defense genes

The rice tapetum-specific gene RA39 encodes a type I ribosome-inactivating protein

Type-1 ribosome-inactivating protein from iris (Iris hollandica var. Professor Blaauw) binds specific genomic DNA fragments

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