Microencapsulation of Lactobacillus acidophilus and Lactobacillus plantarum in Eudragit S100 and alginate chitosan under gastrointestinal and normal conditions

Abstract

The main aim of the present study was the investigation of the viability of two probiotics bacteria of Lactobacillus acidophilus and Lactobacillus plantarum in different environments including simulated gastrointestinal and circumstances namely yogurt. For coating of strains, the double-coating technique was used as the first coating was alginate chitosan and second was Eudragit S100 and the results compared with free-coating cells. the number of free L. acidophilus and L. plantarum were 6.4 × 10 7 ± 3.3 × 10 4 and 7.5 × 10 7 ± 3.5 × 10, respectively, and after 32 days in the last measurement, a sharp decrease in the numbers was observed as they reached to 4.6 × 10 4 ± 0.9 × 10 2 and 1.5 × 10 2 ± 7.9 × 10 1 , respectively. In double-coated condition more number of strains survived as L.

Biopolymer‐Based Multilayer Microparticles for Probiotic Delivery to Colon

“…[78] Alternatively, the preformed microparticles (or pristine bacterial suspension) can be coated with multiple layers of oppositely charged polymers through a layer by layer electrostatic deposition technique. [79,80] The outermost coating layer itself can also be cross-linked (chemically or physically) to bestow better sealing effect to the microparticles. [81] Depending on the coating modality, this technique is capable of fabricating two-, three-, and even four-layer microparticles to endow further protection for the encapsulated probiotics.…”

“…Layer-by-layer self-assembly is a useful modality in the fabrication of three-layer microparticles with sequentially opposingly charged polymers in each layer. [64,78,79,113] Alternatively, twolayer microparticles fabricated through co-extrusion could be further dip-coated with a polymeric outer shell layer with opposite charge. [40,62] Regardless, the choice of biopolymer for the outer shell layer could be either a negatively charged polymer or a positively charged one.…”

Perspective on Constructing Cellulose-Hydrogel-Based Gut-Like Bioreactors for Growth and Delivery of Multiple-Strain Probiotic Bacteria

“…It is well-established that selection of suitable encapsulating material is of prime importance to ensure the effectiveness of probiotics. Over the last decades, studies have been conducted on development of novel and efficient biocomposites for possible application in probiotic encapsulation. ,,,− There have been excellent reviews published in this area; hence, for the sake of brevity and considering the scope of this perspective, we refer the reader to the recent reviews. − Despite progress made in the field, novel approaches toward the encapsulation of probiotics ensuring high viability, efficiency, biocompatibility, and timely and targeted release of probiotic cells represent a field of opportunities that need to be explored.…”

New insights into the role of nanotechnology in microbial food safety

“…Initially, blended mixture of Fig. 3 Images including free coated (a), single chitosan coated (b), double-coated bacteria (c), alginate beads extracted from GI condition in the period of 8, 16 and 32 days after storage (Rahmati 2020) encapsulation materials suspended in probiotic bacteria and oil containing Tween 80. Afterward, the produced product turns into smaller pieces by adding chloride calcium and prepared capsules using a centrifuge.…”

“…The freezing process is followed by a primary drying, wherein shelf temperature usually increased, the chamber pressure is lowered, and unbound H 2 O 2 molecules removed by sublimation. In the second step, it removes the bound water molecules by desorption as the product temperature is progressively reaching the ambient temperature (Rahmati 2020).…”