Microencapsulation of human spermatozoa increases membrane stability and DNA longevity

Gosálvez, Jaime; López‐Fernández, Carmen; Fernández, José Luis Fernández; Johnston, Stephen D.

doi:10.1111/and.13924

Cited by 3 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Seminal plasma-derived and stem cell-derived exosomes were reported to be beneficial to sperm cryopreservation or stored in the liquid state [ 285 , 286 ]. Moreover, alginic acid microencapsulation of human sperm is shown to significantly improve the cumulative membrane integrity and DNA fragmentation following storage for 24 h at 37 °C [ 287 ].…”

Section: Future Perspectivementioning

confidence: 99%

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Cheng

Jiang

et al. 2022

Cells

View full text Add to dashboard Cite

Preservation of human spermatozoa in vitro at normothermia or hypothermia maintaining their functions and fertility for several days plays a significant role in reproductive biology and medicine. However, it is well known that human spermatozoa left in vitro deteriorate over time irreversibly as the consequence of various stresses such as the change of osmolarity, energy deficiency, and oxidative damage, leading to substantial limitations including the need for semen examinations, fertility preservation, and assisted reproductive technology. These problems may be addressed with the aid of non-freezing storage techniques. The main and most effective preservation strategies are the partial or total replacement of seminal plasma with culture medium, named as extenders, and temperature-induced metabolic restriction. Semen extenders consist of buffers, osmolytes, and antioxidants, etc. to protect spermatozoa against the above-mentioned adverse factors. Extended preservation of human spermatozoa in vitro has a negative effect on sperm parameters, whereas its effect on ART outcomes remains inconsistent. The storage duration, temperature, and pre-treatment of semen should be determined according to the aims of preservation. Advanced techniques such as nanotechnology and omics have been introduced and show great potential in the lifespan extension of human sperm. It is certain that more patients will benefit from it in the near future. This review provided an overview of the current knowledge and prospects of prolonged non-freezing storage of human sperm in vitro.

show abstract

Section: Future Perspectivementioning

confidence: 99%

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Cheng

Jiang

et al. 2022

Cells

View full text Add to dashboard Cite

show abstract

Alginate encapsulation of stallion sperm for increasing storage stability

Pruß

Oldenhof

Wolkers

et al. 2022

Animal Reproduction Science

View full text Add to dashboard Cite

Assisted breeding technology in the saltwater crocodile

Johnston

Lever²,

McLeod³

et al. 2021

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

This review reports the current status of artificial breeding technology in the Crocodylia and the future requirements for the establishment of AI in the saltwater crocodile. Although there are challenges regarding safe restraint and immobilisation, semen collection of the saltwater crocodile by manual stimulation has proven effective in yielding sufficient volume and sperm concentrations for empirical and molecular analyses of sperm preservation and physiology. Nevertheless, there is still much to learn with respect to fundamental anatomy, physiology and behaviour in both sexes, but particularly in the female. Although lessons can be learned from successful AI in the alligator, the details of this research are not readily accessible. Future research needs to focus on the proximate factors of seasonality and the underlying control of the female’s annual reproductive cycle; this will require novel and innovative ways to collect blood samples without causing stress or injury, and ideally a dedicated crocodile research breeding colony. Because the saltwater crocodile is a farmed species, there is likely to be sufficient impetus for the application of assisted breeding technology to drive future productivity in the industry. These developments will also have benefits for the genetic and reproductive management of endangered captive populations.

show abstract

Microencapsulation of human spermatozoa increases membrane stability and DNA longevity

Cited by 3 publications

References 27 publications

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Alginate encapsulation of stallion sperm for increasing storage stability

Assisted breeding technology in the saltwater crocodile

Contact Info

Product

Resources

About