Microencapsulation of Bifidobacterium animalis subsp. lactis BB-12 with mannitol

Abstract

Bifidobacterium animalis subsp. lactis BB-12 (BB-12) was microencapsulated using co-extrusion technology with chitosan coating and the incorporation of mannitol as prebiotic. Optimization of coating material chitosan concentration (0–0.5% w/v) and mannitol concentration (0–5% w/v) as prebiotic were performed to determine the formulation that produces beads with desired properties. The microencapsulation efficiency (MEE) of free and microencapsulated BB-12 (with and without mannitol) were determined. All forms of BB-12 further subjected to sequential digestion in simulated gastric juice (SGJ, pH 2.0) for 2 hours and simulated intestinal juice (SIJ, pH 7.5) for 3 hours. The results indicated that 0.4% (w/v) of chitosan coating and 3% (w/v) of mannitol were the optimum concentrations to produce microencapsulated BB-12 with the highest MEE of 89.15% and the average bead size of 805 µm. The BB-12 beads produced through co-extrusion were spherical with a smooth surface. Throughout the five hours sequential gastrointestinal digestion, both microencapsulated BB-12 with and without mannitol were able to maintain their viable cell count at least 106 CFU/g at the end of the incubation. The presence of prebiotic mannitol showed a significant protective effect on the microencapsulated BB-12 during gastrointestinal transit.

Microencapsulation of Lactiplantibacillus plantarum with inulin and evaluation of survival in simulated gastrointestinal conditions and roselle juice

“…According to Table 1, the increase in inulin concentration also had no impact (p > 0.05) on the size of the microbeads. A similar trend was observed by others where the diameter of the microbeads was not affected by different prebiotic concentrations (Chan & Pui, 2020;Haghshenas et al, 2015;Yong et al, 2020). Microencapsulation efficiency of beads using 3.0% (w/v) of inulin was higher as compared to other concentration except for the control.…”

“…B. animalis subsp. lactis encapsulated via co-extrusion technique, using calcium alginate as the wall material and chitosan as the coating material, displayed a smooth and uniform surface (Yong et al, 2020). This showed that the production of microbeads using the co-extrusion technique with calcium alginate and chitosan was able to provide a smooth and uniform texture (Shinde et al, 2014).…”

Storage stability of microencapsulated Lactobacillus rhamnosus GG in hawthorn berry tea with flaxseed mucilage

“…Moreover, the co‐extrusion technique is conducted in a sterile environment with mild conditions, hence it is suitable to be used for sensitive bioactive materials such as probiotics (Shinde et al., 2014; Silva et al., 2016). Prebiotics are often encapsulated with probiotics as prebiotic act as a nutrient for probiotics and give a synergistic therapeutic effect toward humans (Chan & Pui, 2020; Siang et al., 2019; Yong et al., 2020).…”

“…Prebiotics are often encapsulated with probiotics as prebiotic act as a nutrient for probiotics and give a synergistic therapeutic effect toward humans (Chan & Pui, 2020;Siang et al, 2019;Yong et al, 2020).…”

Preliminary evaluation of potential prebiotic capacity of selected legumes and seed mucilage on the probiotic strain Lactobacillus rhamnosus GG

“…Due to increasing health awareness among consumers, the demand for functional foods, namely probiotics and prebiotics has expanded greatly (Lai et al, 2020a;Singla & Chakkaravarthi, 2017;Zaman & Sarbini, 2016). Common prebiotics such as mannitol, isomaltooligosaccharides, and oligofructose had been reported to protect the viability of probiotics (Chan & Pui, 2020;Ng et al, 2019;Siang et al, 2019;Yee et al, 2019;Yong et al, 2020).…”