Microencapsulation‐Based Cell Therapy

Zimmermann, U.; Cramer, Hubert; Jork, Anette; Thürmer, F.; Zimmermann, Heiko; Fuhr, G.; Hasse, Christian; Rothmund, M.

doi:10.1002/9783527620937.ch19

Cited by 15 publications

(16 citation statements)

References 97 publications

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“…Because of their multilineage potential [1], human mesenchymal stem cells are qualified for many applications such as tissue engineering and cell therapy [2][3][4][5][6]. Normally, the cells are expanded before their use.…”

Section: Introductionmentioning

confidence: 99%

Expansion and Harvesting of hMSC-TERT

Weber¹,

Pöhl²,

Pörtner³

et al. 2007

TOBEJ

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Abstract:The expansion of human mesenchymal stem cells as suspension culture by means of spinner flasks and microcarriers, compared to the cultivation in tissue culture flasks, offers the advantage of reducing the requirements of large incubator capacities as well as reducing the handling effort during cultivation and harvesting. Nonporous microcarriers are preferable when the cells need to be kept in viable condition for further applications like tissue engineering or cell therapy. In this study, the qualification of Biosilon, Cytodex 1, Cytodex 3, RapidCell and P102-L for expansion of hMSC-TERT with an associated harvesting process using either trypsin, accutase, collagenase or a trypsin-accutase mixture was investigated. A subsequent adipogenic differentiation of harvested hMSC-TERT was performed in order to observe possible negative effects on their (adipogenic) differentiation potential as a result of the cultivation and harvesting method. The cultivated cells showed an average growth rate of 0.52 d -1 . The cells cultivated on Biosilon, RapidCell and P102-L were harvested succesfully achieving high cell yield and vitalities near 100%. This was not the case for cells on Cytodex 1 and Cytodex 3. The trypsin-accutase mix was most effective. After spinner expansion and harvesting the cells were successfully differentiated to adipocytes.

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Section: Introductionmentioning

confidence: 99%

Expansion and Harvesting of hMSC-TERT

Weber¹,

Pöhl²,

Pörtner³

et al. 2007

TOBEJ

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“…Попередити можливі негативні наслідки пересадки донорського матеріалу, а також виключити необхідність застосування імуносупресивної терапії можна за допомо-гою методу мікроінкапсуляції тканини або клітин в капсули з напівпроникними мембра-нами, які створюють імунологічний бар'єр між трансплантатом та організмом реципієн-та при можливості необмеженої дифузії гор-монів, поживних речовин, кисню, месенжерів і метаболітів [17,18,21]. Для виготовлення мікрокапсул найчастіше застосовують біо-полімер альгінат, який отримують з морських водоростей або вирощують в біореакторі з використанням бактерій [22].…”

Section: вступunclassified

Characteristization of microencapsulated human adrenal cortex tissue in long-term culture

PASTEUR¹,

Levchuk²,

Тronko³

2014

Fiziol Zh

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“…Íàéá³ëüø ïåðñïåêòèâíèì º çàñòî-ñóâàííÿ àëüã³íàòó, ÿêèé îòðèìóþòü ç ìîð-ñüêèõ âîäîðîñòåé àáî âèðîùóþòü ó á³î-ðåàêòîð³ ç âèêîðèñòàííÿì ñïåö³àëüíèõ áàê-òåð³é [21]. Àëüã³íàò ñêëàäàºòüñÿ ç íåðîçãà-ëóaeåíèõ ïîäâ³éíèõ ãîìî-³ ãåòåðîïîë³ìåð-íèõ 1-4-çâÿçàííèõ β-D-ìàíóðîíîâî¿ òà α-L-ãóëóðîíîâî¿ êèñëîò ð³çíîìàí³òíèõ çà ñêëàäîì ³ ïîñë³äîâí³ñòþ çàëåaeíî â³ä äaeå-ðåëà ïîõîäaeåííÿ [13,20].…”

Section: âñòóïmentioning

confidence: 99%