Microencapsulated chitosan nanoparticles for lung protein delivery

Grenha, Ana; Seijo, Begoña; Remuñán‐López, Carmen

doi:10.1016/j.ejps.2005.04.009

Cited by 409 publications

(285 citation statements)

References 36 publications

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“…All these properties, place chitosan as a potential alternative to administrate drugs to or through the lung. There are reports showing the effectiveness of chitosan increasing lung retention of drugs (Grenha et al, 2005;Li and Birchall, 2006;Teijeiro-Osorio et al, 2009). …”

Section: Discussionmentioning

confidence: 99%

Chitosan as a non-viral co-transfection system in a cystic fibrosis cell line

Fernández

Santos-Carballal

Weber

et al. 2016

International Journal of Pharmaceutics

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Successful gene therapy requires the development of suitable vehicles for the selective and efficient delivery of genes to specific target cells at the expense of minimal toxicity. In this work, we investigated a non-viral gene delivery system based on chitosan (CS) to specifically address cystic fibrosis (CF). Thus, electrostatic self-assembled CS-pEGFP and CS-pEGFPsiRNA complexes were prepared from high-pure fully characterized CS (Mw ~20 kDa and degree of acetylation ~30%). The average diameter of positively-charged complexes (i.e.  ~ +25 mV) was ~200 nm. The complexes were found relatively stable over 14 h in OptiMEM. Cell viability study did not show any significant cytotoxic effect of the CS-based complexes in a human bronchial cystic fibrosis cell line (s). We evaluated the transfection efficiency of this cell line with both CS-pEGFP and co-transfected with CS-pEGFP-siRNA complexes at (N/P) charge ratio of 12. We reported an increase in the fluorescence intensity of CS-pEGFP and a reduction in the cells co-transfected with CS-pEGFP-siRNA. This study shows proof-of-principle that co-transfection with chitosan might be an effective delivery system in a human CF cell line. It also offers a potential alternative to further develop therapeutic strategies for inherited disease treatments, such as CF.

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Section: Discussionmentioning

confidence: 99%

Chitosan as a non-viral co-transfection system in a cystic fibrosis cell line

Fernández

Santos-Carballal

Weber

et al. 2016

International Journal of Pharmaceutics

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“…CS and AP were used at a fixed concentration of 1 mg/ml in each formulation and the second polymer was added in variable concentrations (the amount decreasing from 1/2 to 1/4) to obtain the different mass ratios. Therefore, as the formulations with mass ratio 1/2 are those integrating the higher amount of negative polymer, a higher degree of interaction between negatively and positively charged groups is potentiated, leading to the formation of a higher number of nanoparticles (Fernández-Urrusuno et al, 1999b;Grenha et al, 2005;Rodrigues et al, 2012b). In fact, in SP/CS formulations, positive to negative (+/-) charge ratios vary from 2 to 4, and in CRG/AP formulations, from 3 to 6, as the amount of negatively charged polymer decreases.…”

Section: Preparation and Characterisation Of Nanoparticlesmentioning

confidence: 99%

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Dionísio

Cordeiro

Remuñán‐López

et al. 2013

European Journal of Pharmaceutical Sciences

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Polymeric nanoparticles have revealed very effective in transmucosal delivery of proteins. Polysaccharides are among the most used materials for the production of these carriers, owing to their structural flexibility and propensity to evidence biocompatibility and biodegradability. In parallel, there is a preference for the use of mild methods for their production, in order to prevent protein degradation, ensure lower costs and easier procedures that enable scaling up.In this work we propose the production of pullulan-based nanoparticles by a mild method of polyelectrolyte complexation. As pullulan is a neutral polysaccharide, sulfated and aminated derivatives of the polymer were synthesized to provide pullulan with a charge. These derivatives were then complexed with chitosan and carrageenan, respectively, to produce the nanocarriers. Positively charged nanoparticles of 180-270 nm were obtained, evidencing ability to associate bovine serum albumin, which was selected as model protein. In PBS pH 7.4, pullulan-based nanoparticles were found to have a burst release of 30% of the protein, which maintained up to 24h. Nanoparticle size and zeta potential were preserved upon freeze-drying in the presence of appropriate cryoprotectants. A factorial design was approached to assess the cytotoxicity of raw materials and nanoparticles by the metabolic test MTT. Nanoparticles demonstrated to not cause overt toxicity in a respiratory cell model (Calu-3). Pullulan has, thus, demonstrated to hold potential for the production of nanoparticles with an application in protein delivery.

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“…These properties include: biodegradability, lack of toxicity, antibacterial and antifungal effects, wound healing acceleration and immune system stimulation (Shi et al, 2011a). Because of excellent biological and physico-chemical properties of chitosan, it has the ability to bind to particular materials including cholesterols, fats, proteins, metal ions and even tumor cells (Grenha et al, 2005). This allows chitosan to be used as a chelating agent in various applications.…”

Section: Applications Of Chitosanmentioning

confidence: 99%

“…It is a linear polyamine containing a number of free amine groups that are readily available for cross linking and its cationic nature allows for ionic cross linking with multivalent anions. Chitosan has mucoadhesive character, which increases residual time at the site of absorption (Agnihotri et al, Science Publications AJBB 2004; Grenha et al, 2005;Kim et al, 2008;Racovita et al, 2008). Chemical structures of chitin and chitosan are shown in Fig.…”

Section: Chitosanmentioning

confidence: 99%

Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

Malmiri

Jahanian

Berenjian

2012

American Journal of Biochemistry and Biotechnology

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Chitosan is an attractive natural biopolymer from renewable resources with the presence of reactive amino and hydroxyl functional groups in its structure. Due to the good biocompatibility of chitosan, it can be used in magnetic-field assisted drug delivery, enzyme or cell immobilization and many other industrial applications. In the past decade, nanotechnology has been a considerable research interest in the area of preparation of immobilized enzyme carriers. This study looks at characteristics and applications of chitosan and chitosan nanoparticles and their potentials as suitable supports for enzyme immobilization. Results indicated that activity of immobilized enzymes and performance of enzyme immobilization onto chitosan nanoparticles are higher than chitosan macro and microparticles. As compared to other biopolymers nanoparticles, application of chitosan nanoparticles to immobilize enzymes strongly increases stability of immobilized enzymes and their easy separability from the reaction mixture at the end of the biochemical process.

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Microencapsulated chitosan nanoparticles for lung protein delivery

Cited by 409 publications

References 36 publications

Chitosan as a non-viral co-transfection system in a cystic fibrosis cell line

Chitosan as a non-viral co-transfection system in a cystic fibrosis cell line

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

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