Microemulsion electrokinetic chromatography: comparison with micellar electrokinetic chromatography

Terabe, Shigeru; Matsubara, Naoko; Ishihama, Yasushi; Okada, Yukihiro

doi:10.1016/0021-9673(92)87102-e

Cited by 160 publications

(76 citation statements)

References 21 publications

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“…After the first report on the use of a microemulsion as a running buffer in CE, an increasing number of papers describing different applications of microemulsion electrokinetic chromatography (MEEKC) were published. MEEKC has been shown to be useful for the separation of very lipophilic substances such as polyaromatic hydrocarbons (Watarai, 1991;Altria, 2000) and fat-soluble vitamins (Altria, 1999;PedersenBjergaard et al, 2000), and very hydrophilic substances like water-soluble vitamins (Boso et al, 1995;Altria, 1999) and proteins (Zhou et al, 1999), as well as pharmaceuticals (Terabe et al, 1992;Altria, 1999) and natural products (Debusschère et al, 1997;Li et al, 1998).…”

Section: Introductionmentioning

confidence: 98%

Microemulsion electrokinetic chromatography with laser‐induced ﬂuorescence detection: as tested with amino acid derivatives

Xie¹,

Zhang²,

Liu³

et al. 2004

Biomedical Chromatography

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Over a decade ago, microemulsion electrokinetic chromatography was introduced as a novel mode of capillary electrophoresis. However, there has not been publication on the combination of microemulsion electrokinetic chromatography with laser-induced fluorescence detection. In this paper, a preliminary method using microemulsion eletrokinetic chromatography combined with laser-induced fluorescence detection and second derivative electrophoregram was established as a sensitive and selective assay for separation and determination of nine amino acids after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazol. The derivatization and separation conditions were optimized. In the investigated concentration ranges correlation coefficients were better than 0.995. The relative standard deviation (n = 5) of the migration times and peak heights were 0.56-0.76 and 2.21-7.15%, respectively. The detection limits (S/N = 3) were at a neaomolar level (0.32-2.20 nM). The method was applied for the analysis of compound amino acid injection and a Chinese traditional herbal medicine. The recoveries were 95.9-107.9%.

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Section: Introductionmentioning

confidence: 98%

Microemulsion electrokinetic chromatography with laser‐induced ﬂuorescence detection: as tested with amino acid derivatives

Xie¹,

Zhang²,

Liu³

et al. 2004

Biomedical Chromatography

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“…Shohat and Grushka [15] employed p-sulfonatocalix [6]arene to resolve chlorinated phenols and benzenediols. Recently Sepaniak et al [16] investigated the use of p-(carboxyethyl)calix[n]arenes (with n = 4, 5,7,8) for the separation of small and polar-substituted polycyclic aromatic hydrocarbons (PAHs).…”

Section: Introductionmentioning

confidence: 99%

Discontinuous electrokinetic chromatography of parabens using different substituted resonances as pseudostationary phases

et al. 1999

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“…Although being virtually resistant to clogging and offering exceedingly high separation efficiencies, CE suffers from other drawbacks such as an incapability of separating analytes with identical size to charge ratio. Capillary electrochromatography (CEC) [9], micellar electrokinetic chromatography (MEKC) [10], and microemulsion electrokinetic chromatography (MEEKC) [11] are modifications of CE that have mainly been developed to enable separation of isomers and neutrals. Thus, with these techniques charged analytes and analytes having the same charge-to-size ratio can be simultaneously separated based on the combination of electrophoresis and chromatography.…”

Section: Introductionmentioning

confidence: 99%

Continuous full filling capillary electrochromatography–electrospraying chromatographic nanoparticles

et al. 2010

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Abbreviations CFFContinuous full filling IS AbstractThe influence of instrumental parameters affecting the ionization in continuous full-filling capillary electrochromatography / electrospray ionization mass spectrometry (CFF-CEC/ESI-MS) was investigated. The investigated parameters were the background electrolyte (BGE) and sheath liquid ion strength and organic modifier content, the nebulizer gas pressure, and the concentration of nanoparticles in the BGE. It was found that the nebulizer pressure had the largest influence on the separation efficiency and apparent retention. It was shown that even the lowest pressure investigated was sufficient to guide the nanoparticle flow away from the mass spectrometer inlet. A nebulizer pressure of 5 psi was found being optimal; increasing the pressure significantly decreased the separation efficiency due to the generation of a hydrodynamic flow. Generally, the ion strength of both the BGE and the sheath liquid were found to have very moderate effects on the separation of a homologous series of dialkyl phthalates. Whereas the ionization efficiency was found being unaffected by the nanoparticles, the separation efficiency was found to increase with increasing concentrations up to 3.8 mg/mL, where after it was observed to drop. The optimized method was linear over a wide concentration range and presented limits of detection and quantification more than three-fold lower than what have previously been reported using CFF-CEC/ESI-MS.3

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Microemulsion electrokinetic chromatography: comparison with micellar electrokinetic chromatography

Cited by 160 publications

References 21 publications

Microemulsion electrokinetic chromatography with laser‐induced ﬂuorescence detection: as tested with amino acid derivatives

Microemulsion electrokinetic chromatography with laser‐induced ﬂuorescence detection: as tested with amino acid derivatives

Discontinuous electrokinetic chromatography of parabens using different substituted resonances as pseudostationary phases

Continuous full filling capillary electrochromatography–electrospraying chromatographic nanoparticles

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