Microemulsion-Based Oxyresveratrol for Topical Treatment of Herpes Simplex Virus (HSV) Infection: Physicochemical Properties and Efficacy in Cutaneous HSV-1 Infection in Mice

Abstract

Abstract. The physicochemical properties of the optimized microemulsion and the permeating ability of oxyresveratrol in microemulsion were evaluated, and the efficacy of oxyresveratrol microemulsion in cutaneous herpes simplex virus type 1 (HSV-1) infection in mice was examined. The optimized microemulsion was composed of 10% w/w of isopropyl myristate, 35% w/w of Tween 80, 35% w/w of isopropyl alcohol, and 20% w/w of water. The mean particle diameter was 9.67±0.58 nm, and the solubility of oxyresveratrol in the microemulsion was 196.34±0.80 mg/ml. After accelerated and long-term stability testing, the microemulsion base and oxyresveratrol-loaded microemulsion were stable. The cumulative amount of oxyresveratrol permeating through shed snake skin from microemulsion at 6 h was 93.04 times compared to that of oxyresveratrol from Vaseline, determined at 20% w/w concentration. In cutaneous HSV-1 infection in mice, oxyresveratrol microemulsion at 20%, 25%, and 30% w/w, topically applied five times daily for 7 days after infection, was significantly effective in delaying the development of skin lesions and protecting from death (p<0.05) compared with the untreated control. Oxyresveratrol microemulsion at 25% and 30% w/w was significantly more effective than that of 30% w/w of oxyresveratrol in Vaseline (p<0.05) and was as effective as 5% w/w of acyclovir cream, topically applied five times daily (p>0.05). These results demonstrated that topical oxyresveratrol microemulsion at 20-30% w/w was suitable for cutaneous HSV-1 mouse infection.

Testing of resveratrol microemulsion photostability and protective effect against UV induced oxidative stress

“…Similar effects of active substances on droplet size were reported previously when active substances were incorporated into colloidal systems, e.g. microemulsions (14), liposomes (10), nanosuspensions (15). The possibility of RES accumulation in the interfacial layer of nanostructure systems has been discussed earlier (6).…”

Therapeutic efficacy in BALB/C mice of extract from marine alga Canistrocarpus cervicornis (Phaeophyceae) against herpes simplex virus type 1

“…In this study, the ointment (lanovaseline) vehicle was better to solubilize the extract (2 %), and oral administration has not been performed by the low solubility in vehicles with low toxicity such as water and DMSO 1 %. Studies using oxyresveratrol derived from the traditional Thai plant, Artocarpus lakoocha Roxburgh, demonstrated that oxyresveratrol in cream base at 10 % applied three to five times daily (Lipipun et al 2011) and in oxyresveratrol microemulsion at 20-30 % w/w applied five times daily against HSV-1 for 7 days (Sasivimolphan et al 2012) were suitable for cutaneous HSV-1 mouse infection.…”

Encapsulation artocarpanone and ascorbic acid in O/W microemulsions: Preparation, characterization, and antibrowning effects in apple juice

“…The values of polydispersity indices (PdI) were 0.21 ± 0.01, 0.26 ± 0.01, and 0.25 ± 0.00 for BME, ArtoME, and Arto + VcME, respectively. The values of PdI were low, which implied that the formulas had uniformity of the narrow size distribution (Sasivimolphan et al, 2012). The conductivity values of BME and ArtoME were 135.01 ± 0.22 and 160.32 ± 1.91 lS/cm, respectively.…”

“…Pseudo-ternary phase diagrams were constructed using the water titration method to obtain the suitable concentration range of components which formed microemulsion (Sasivimolphan et al, 2012). To investigate the area of ME region, surfactant were mixed with cosurfactant at a weight ratio (S mix ) of 1:1, 2:1, 3:1, and 4:1.…”

“…Pseudo-ternary phase diagrams were constructed in order to figure out the optimal proportion of the components in MEs, which were constructed using water titration method (Bergonzi, Hamdouch, Mazzacuva, Isacchi, & Bilia, 2014;Sasivimolphan et al, 2012).…”