Microelectrode arrays and application to biosensing devices

Hintsche, R.; Paeschke, Manfred; Wollenberger, Ulla; Schnakenberg, Uwe; Wagner, B.; Lisec, T.

doi:10.1016/0956-5663(94)80068-5

Cited by 85 publications

(42 citation statements)

References 18 publications

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“…Alkaline phosphatase (ALP) cleaving p-aminophenyl phosphate under formation of the electroactive p-aminophenol is a widely used labeling compound [8,9]. Typically, interdigitated electrode arrays (IDA, [10,11,12]) are used for such sensor architectures [13,14,15] and the efficiency of amplification is strongly dependent on the spacing of the two electrodes [11,12,16]. The closer the electrodes, the higher the amplification obtained by the structure used for the biosensor.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Nanopore Electrode Structures as Basis for Amplified Electrochemical Assays

et al. 2006

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A nanopore electrode structure was fabricated consisting of ensembles of nanopores with separately addressable electrodes at the pore bottoms and the rims. A metal/insulator/metal layer structure allowed for adjusting the spacing between the bottom and rim electrodes to be in the range of about 200 nm. Pore diameters varied between 200 and 800 nm. The electrochemical properties of this electrode structure and its perspectives for applications in bioelectronics were studied using cyclic voltammetry and chronoamperometry along with high-resolution scanning electrochemical microscopy (SECM) in constant-distance mode. It was possible to visualize the electrochemical activity of a single nanometric electrode using high-resolution SECM in a combination of sample-generation-tipcollection mode and positive feedback mode. The SECM images suggested an influence of the unbiased rim electrode on redox amplification which was used as a basis for evaluating the feasibility of current amplification by means of redox cycling between the bottom and rim electrodes. Amplification factors superior to those obtained with interdigitated array electrodes could be demonstrated.

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Section: Introductionmentioning

confidence: 99%

Characterization of Nanopore Electrode Structures as Basis for Amplified Electrochemical Assays

et al. 2006

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“…For example, they demonstrate steady state currents, higher sensitivity caused by the increased mass transport and application to highly resistant solutions. Additionally, UMEs have been applied as sensors in various techniques such as flow injection analysis [12,13], cardiovascular monitoring and organic compounds analysis [14][15][16]. For this work, we used the highly developed version of a classical cyclic voltammetric technique combined with fast Fourier transformation method (the computer program is more advanced in the algorithm for integration and filtering).…”

Section: Scheme 1 Chemical Structure Of Capmentioning

confidence: 99%

Fast Fourier transformation with continuous cyclic voltammetry at an Au microelectrode for the determination of morphine in a flow injection system

Norouzi

Ganjali

Moosavi-Movahedi

et al. 2007

Talanta

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Fast Fourier transformation with continuous cyclic voltammetry (FFTCV) in a flowing solution was applied as a detection system for the prompt Chloramphenicol (CAP) monitoring. In this method, the potential waveform consisted of two potential sections for cleaning of the electrode surface, accumulating CAP and potential ramp, which was continuously applied on a Pt-Au dual disk microelectrodes. The effects of various parameters on the sensitivity of the method were investigated. When the optimum conditions were imposed, this is a scan rate value of 70 V/s, an accumulation time of 500 ms, an accumulation potential of -300 mV and a pH value of 2. The method proved to be linear over the concentration range of 8.0 ×10 -9 -2.0 ×10 -5 mol/L (r = 0.9981) with a detection limit and a quantitation limit of 2.0 ×10 -9 and 6.6×10 -9 mol/L, respectively. Consequently, the method illustrates the requisite accuracy, sensitivity, precision and selectivity to assay CAP in its biological fluids. The proposed method has some advantages over other reported methods such as, no need for the removal of oxygen from the test solution, a sub-nanomolar detection limit, and finally the method is fast enough for the determination of any such compound, in a wide variety of chromatographic methods. As a result, FFTCV illustrates the benefits of precision, determination speed, cost-effectiveness, accuracy and simplicity, in comparison with formerly reported techniques.

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“…3,4 UMEs (ultra microelectrodes), for instance, have been applied as sensors in various techniques such as flow injection analysis, 5,6 organic compound analysis. 7,8 Now, our work describes a new electrochemical method based on FIA and FFT cyclic voltammetry for determination of letrozole.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of Anti Cancer Drug Letrozole by Fast Fourier Transform Continuous Cyclic Voltammetry at Gold Microelectrode

et al. 2010

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A continuous cyclic voltammetric study of letrozole at gold microelectrode was carried out. The drug in phosphate buffer (pH 2.0) is adsorbed at -200 mV, giving rise to change in the current of well-defined oxidation peak of gold in the flow injection system. The proposed detection method has some of advantages, the greatest of which are as follows: first, it is no more necessary to remove oxygen from the analyte solution and second, this is a very fast and appropriate technique for determination of the drug compound in a wide variety of chromatographic analysis methods. Signal-to-noise ratio has significantly increased by application of discrete Fast Fourier Transform (FFT) method, background subtraction and two-dimensional integration of the electrode response over a selected potential range and time window. Also in this work some parameters such as sweep rate, eluent pH, and accumulation time and potential were optimized. The linear concentration range was of 1.0×10-7 -1.0×10 -10 mol/L (r＝0.9975) with a limit of detection and quantitation 0.08 nmol/L and 0.15 nmol/L, respectively. The method has the requisite accuracy, sensitivity, precision and selectivity to assay letrozol in tablets. The influences of pH of eluent, accumulation potential, sweep rate, and accumulation time on the determination of the letrozol were considered.

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Microelectrode arrays and application to biosensing devices

Cited by 85 publications

References 18 publications

Characterization of Nanopore Electrode Structures as Basis for Amplified Electrochemical Assays

Characterization of Nanopore Electrode Structures as Basis for Amplified Electrochemical Assays

Fast Fourier transformation with continuous cyclic voltammetry at an Au microelectrode for the determination of morphine in a flow injection system

Monitoring of Anti Cancer Drug Letrozole by Fast Fourier Transform Continuous Cyclic Voltammetry at Gold Microelectrode

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