Microelectrochemical visualization of oxygen consumption of single living cells

Nebel, Michaela; Grützke, Stefanie; Diab, Nizam; Schulte, Albert; Schuhmann, Wolfgang

doi:10.1039/c3fd00011g

Cited by 37 publications

(29 citation statements)

References 64 publications

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“…Unmodified barrels, filled with electrolyte solution, can be used for SICM distance control. This channel allows the positioning of nanoprobes with sub-cellular resolution and would allow the mapping of oxygen consumption outside living cell 42 or the functional study of mitochondrial respiration. 50 …”

Section: Resultsmentioning

confidence: 99%

Electrochemical Nanoprobes for Single-Cell Analysis

et al. 2014

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The measurement of key molecules in individual cells with minimal disruption to the biological milieu is the next frontier in single-cell analyses. Nanoscale devices are ideal analytical tools because of their small size and their potential for high spatial and temporal resolution recordings. Here, we report the fabrication of disk-shaped carbon nanoelectrodes whose radius can be precisely tuned within the range 5-200 nm. The functionalization of the nanoelectrode with platinum allowed the monitoring of oxygen consumption outside and inside a brain slice. Furthermore, we show that nanoelectrodes of this type can be used to impale individual cells to perform electrochemical measurements within the cell with minimal disruption to cell function. These nanoelectrodes can be fabricated combined with scanning ion conductance microcopy probes which should allow high resolution electrochemical mapping of species on or in living cells.

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Section: Resultsmentioning

confidence: 99%

Electrochemical Nanoprobes for Single-Cell Analysis

et al. 2014

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“…As a result, the conditions for the best contrast of a SECM experiment are chosen after the SECM investigation is completed without the demand of knowing the exact contribution of each parameter beforehand. Moreover, we recently demonstrated that the reaction at a continuously polarized tip may actively influence the detection of the local oxygen concentration inside the gap between tip and sample [38–39]. As a consequence, the oxygen detection is superimposed by effects caused by the tip reaction and the tip is not acting as a passive spectator.…”

Section: Resultsmentioning

confidence: 99%

Constant-distance mode SECM as a tool to visualize local electrocatalytic activity of oxygen reduction catalysts

Nebel¹,

Erichsen²,

Schuhmann³

2014

Beilstein J. Nanotechnol.

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SummaryMultidimensional shearforce-based constant-distance mode scanning electrochemical microscopy (4D SF/CD-SECM) was utilized for the investigation of the activity distribution of oxygen reduction catalysts. Carbon-supported Pt model catalyst powders have been immobilized in recessed microelectrodes and compared to a spot preparation technique. Microcavities serve as platform for the binder-free catalyst sample preparation exhibiting beneficial properties for constant-distance mode SECM imaging concerning modified surface area and catalyst loading. The integration of the redox competition mode of SECM into the detection scheme of the 4D SF/CD mode is demonstrated for specifically adapting high-resolution SECM experiments to powder-based catalyst preparations.

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“…Electrochemical methods are very attractive due to their low-capital investment requirements and microfluidic compatibility (Trouillon et al, 2013; D. Kim et al, 2011; Lama et al, 2012; Nebel et al, 2013b). Herein, our focus will be on single cell respiration since oxygen – a common electron acceptor in metabolism – is rather electroactive and thus readily detectable.…”

Section: Probing Single Cellsmentioning

confidence: 99%

Review of methods to probe single cell metabolism and bioenergetics

Vasdekis

Stephanopoulos

2015

Metabolic Engineering

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Single cell investigations have enabled unexpected discoveries, such as the existence of biological noise and phenotypic switching in infection, metabolism and treatment. Herein, we review methods that enable such single cell investigations specific to metabolism and bioenergetics. Firstly, we discuss how to isolate and immobilize individuals from a cell suspension, including both permanent and reversible approaches. We also highlight specific advances in microbiology for its implications in metabolic engineering. Methods for probing single cell physiology and metabolism are subsequently reviewed. The primary focus therein is on dynamic and high-content profiling strategies based on label-free and fluorescence microspectroscopy and microscopy. Non-dynamic approaches, such as mass spectrometry and nuclear magnetic resonance, are also briefly discussed.

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Microelectrochemical visualization of oxygen consumption of single living cells

Cited by 37 publications

References 64 publications

Electrochemical Nanoprobes for Single-Cell Analysis

Electrochemical Nanoprobes for Single-Cell Analysis

Constant-distance mode SECM as a tool to visualize local electrocatalytic activity of oxygen reduction catalysts

Review of methods to probe single cell metabolism and bioenergetics

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