Microdistribution and quantification of the boron neutron capture therapy drug BPA in primary cell cultures of human glioblastoma tumour by NanoSIMS

Aldossari, Samar; McMahon, Greg; Lockyer, Nicholas P.; Moore, Katie L.

doi:10.1039/c9an01336a

Cited by 15 publications

(25 citation statements)

References 55 publications

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“…It is one of the crucial factors, often marked as a drawback in BNCT, because it causes ambiguity in the calculated dose distributions. For effective BNCT treatment, 10 B concentration ratio in the tumor and its concentration in normal tissues (T/N ratio) should be 3:1 or more, and the 10 B concentration in the target should be at least ∼15-30 μg g −1 or ∼10 9 atoms per cell to execute lethal tumor cell damage [63]. Summarizing, to avoid unfavorable effects, the concentration of 10 B in tumor cells and normal tissues must be known.…”

Section: Methods For Assessment Of Boron Concentration In Residual Tumor Volume and Healthy Tissuementioning

confidence: 99%

“…Two directions of studies focused on using SIMS in BNCT can be distinguished: (I) microprobe methods combined with laser post-ionization techniques, and (II) use of the ion microscope technique applying a high-current, primary beam O2 + and afterward with use of position-sensitive detector detecting positive secondary ions [63].…”

Section: Mass Spectrometry Imagingmentioning

confidence: 99%

“…Aldossari et al [79] in their study also conducted an application study for the localization and quantification of therapeutic levels of the BNCT agent L-para-(dihydroxyboryl)-phenylalanine (BPA) in a primary cell with the use of a high-resolution dynamic SIMS instrument. Cell cultures were obtained from patients (humans) who suffered from glioblastoma multiforme tumors.…”

Section: Mass Spectrometry Imagingmentioning

confidence: 99%

See 2 more Smart Citations

Physical bases of Boron Neutron Capture Therapy, Dosimetry, and Its Mechanisms of Action - Critical Overview of the Literature

Skwierawska¹,

Balcerzyk²,

López-Valverde³

et al. 2021

Preprint

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The success of boron neutron capture therapy(BNCT) mainly depends on sufficient spatial bio-distribution of boron(10B) localized around or within the neoplastic cells to produce a high dose gradient between the tumor and healthy tissue. Contrary to what is usual in radiotherapy, BNCT proposes treatment planning directed at the cell instead of the tumor mass. However, it is not yet possible to precisely determine the concentration of 10B in a specific tissue in real-time using non-invasive methods. Some critical issues still need to be resolved if BNCT is to become valuable, minimally invasive, and efficient cancer treatment. This review article provides an overview of fundamental principles, the recent advances, and future directions of BNCT as cellular targeted cancer therapy. The main emphasis is on topics related to biological dosimetry, methods for as-sessment of boron concentration, mechanisms of action of BNCT, and its physical bases for clinical implementation.

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Section: Methods For Assessment Of Boron Concentration In Residual Tumor Volume and Healthy Tissuementioning

confidence: 99%

Section: Mass Spectrometry Imagingmentioning

confidence: 99%

Section: Mass Spectrometry Imagingmentioning

confidence: 99%

See 1 more Smart Citation

Physical bases of Boron Neutron Capture Therapy, Dosimetry, and Its Mechanisms of Action - Critical Overview of the Literature

Skwierawska¹,

Balcerzyk²,

López-Valverde³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Two directions of studies focused on SIMS use in BNCT can be distinguished: (I) microprobe methods combined with post-ionization laser techniques, and (II) use of the ion microscope technique applying a high current primary beam O2 + and afterward with the use of position-sensitive detector detecting positive secondary ions [54].…”

Section: Mass Spectrometry Imagingmentioning

confidence: 99%

“…Aldossari et al [54] in their study also conducted an application study for the localization and quantification of therapeutic levels of the BNCT agent L-para-(dihydroxyboryl)-phenylalanine (BPA) in a primary cell using a high-resolution dynamic SIMS instrument. Cell cultures were obtained from patients (humans) who suffered from glioblastoma multiforme tumors.…”

Section: Mass Spectrometry Imagingmentioning

confidence: 99%

Physical Bases of Boron Neutron Capture Therapy, Dosimetry, and Its Mechanisms of Action—A Critical Overview of the Literature

Skwierawska¹,

Balcerzyk²,

López-Valverde³

et al. 2021

Preprint

View full text Add to dashboard Cite

The success of boron neutron capture therapy (BNCT) depends mainly on sufficient spatial biodistribution of boron (B-10) localized around or within neoplastic cells to produce a high dose gradient between the tumor and healthy tissue. Contrary to what is usual in radiotherapy, BNCT proposes treatment planning directed at the cell rather than the tumor mass. However, it is not yet possible to precisely determine the concentration of B-10 in a specific tissue in real-time using non-invasive methods. Some critical issues still need to be resolved if BNCT is to become a valuable, minimally invasive, and efficient cancer treatment. This review article provides an overview of the funda-mental principles, recent advances, and future directions of BNCT as a cell-targeted cancer therapy. The main emphasis is on topics related to biological dosimetry, methods for assessing boron concentration, mechanisms of action of BNCT, and its physical bases for clinical implementation.

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Measurement of an evaporation coefficient in tissue sections as a correction factor for 10B determination

Espector

Portu

Espain

et al. 2023

Histochem Cell Biol

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Boron Neutron Capture Therapy (BNCT) is a cancer treatment option that combines preferential uptake of a boron compound in tumor and irradiation with thermal neutrons. For treatment planning, boron concentration in different tissues must be considered. Neutron Autoradiography using Nuclear Track Detectors (NTD) can be applied to study both boron concentration and microdistribution in tissue samples. Histological sections are obtained from frozen tissue by cryosectioning. When they reach room temperature, they undergo an evaporation process, which leads to an increase of boron concentration in the sample. In order to take this effect into account, certain correction factors (Evaporation Coe cients, CEv) must be applied.With this aim, a protocol was established in order to register and analyze mass variation of tissue sections, measured with a semimicro scale. Values of ambient temperature, pressure and humidity were simultaneously recorded. Reproducible results of evaporation curves and CEv values were obtained for different tissue samples, which allowed the systematization of the procedure. This study contributes to a more precise determination of boron concentration in tissue samples through the Neutron Autoradiography technique, which is of great relevance to make dosimetric calculations in BNCT.

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Microdistribution and quantification of the boron neutron capture therapy drug BPA in primary cell cultures of human glioblastoma tumour by NanoSIMS

Abstract: The ability of secondary ion mass spectrometry (SIMS) to provide high sensitivity imaging of elements and small-medium mass molecules in biological tissues and cells, makes it a very powerful tool for drug distribution studies.

Cited by 15 publications

References 55 publications

Physical bases of Boron Neutron Capture Therapy, Dosimetry, and Its Mechanisms of Action - Critical Overview of the Literature

Physical bases of Boron Neutron Capture Therapy, Dosimetry, and Its Mechanisms of Action - Critical Overview of the Literature

Physical Bases of Boron Neutron Capture Therapy, Dosimetry, and Its Mechanisms of Action—A Critical Overview of the Literature

Measurement of an evaporation coefficient in tissue sections as a correction factor for 10B determination

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