Microdialysis: A New Technique to Monitor Perioperative Human Peritoneal Mediator Production

Riese, Jutta; Boecker, S; Hohenberger, Werner; Klein, Peter; Haupt, W

doi:10.1089/109629603764655236

Cited by 15 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite the size‐limited passage through the micropores in clinically used filters several studies have shown that cytokines can be evaluated using microdialysis. In particular IL‐1 β and IL‐6 have been studied [4–8], but also MCP‐1 [9] and tumour necrosis factor (TNF)‐ α [10, 11], the latter limited to animal studies. Recovery is only occasionally examined in these studies and when reported it varies from below 1% to up to 50% depending upon the conditions used.…”

Section: Introductionmentioning

confidence: 99%

Microdialysis for Monitoring Inflammation: Efficient Recovery of Cytokines and Anaphylotoxins Provided Optimal Catheter Pore Size and Fluid Velocity Conditions

Wælgaard¹,

Pharo

Tønnessen

et al. 2006

Scand J Immunol

View full text Add to dashboard Cite

Microdialysis emerges as a useful tool to evaluate tissue inflammation in a number of clinical conditions, like sepsis and transplant rejection, but systematic methodological studies are missing. This study was undertaken to determine the recovery of relevant inflammatory mediators using the microdialysis system, comparing microdialysis membranes with two different molecular weight cut‐offs at different flow rates. Twenty and 100 kDa pore sizes CMA microdialysis catheters were investigated using velocities of 0.3, 1.0 and 5.0 μl/min. Reference preparations for cytokines [tumour necrosis factor (TNF)‐α, interleukin (IL)‐1β, IL‐6 and IL‐10; m.w. 17–28 kDa] and chemokines (IL‐8, MCP‐1, IP‐10 and MIG; m.w. 7–11 kDa) were prepared from plasma after incubating human whole blood with lipopolysaccharide. Reference preparation for complement anaphylatoxins (C3a, C4a, C5a; m.w. 9–11 kDa) was prepared by incubating human plasma with heat‐aggregated immunoglobulin G. The reference preparations were quantified for the respective inflammatory molecules and used as medium for the microdialysis procedure. Through the 20 kDa filter only the four chemokines passed, but with low recovery (3–7%) and limited to the 1.0 μl/min velocity. The recovery with the 100 kDa filter was as follows: IL‐1β = 75%, MCP‐1 = 55%, MIG = 50%, IL‐8 = 38%, C4a = 28%, IP‐10 = 22%, C5a = 20%, C3a = 16%, IL‐6 = 11, IL‐10 = 8% and TNF‐α = 4%. The highest recovery for all chemokines and anaphylatoxins were consistently at velocity 1.0 μl/min, whereas IL‐1β and IL‐10 recovered most efficiently at 0.3 μl/min. Thus, microdialysis using catheters with a cut‐off of 100 kDa is a reliable method to detect inflammation as judged by a defined panel of inflammatory markers. These findings may have important implications for future clinical studies.

show abstract

Section: Introductionmentioning

confidence: 99%

Microdialysis for Monitoring Inflammation: Efficient Recovery of Cytokines and Anaphylotoxins Provided Optimal Catheter Pore Size and Fluid Velocity Conditions

Wælgaard¹,

Pharo

Tønnessen

et al. 2006

Scand J Immunol

View full text Add to dashboard Cite

show abstract

“…Microdialysis is a reliable technique for investigating the kinetics of antibiotics in different tissues, and it has been used on many occasions in various tissues, such muscle, lung, brain, and skin, in both animals and humans (18). However, no microdialysis antibiotic-distribution study has ever been conducted in intraperitoneal fluid to our knowledge, although the technique has been used to monitor intraperitoneal concentrations of markers of ischemia, such as lactate, mainly in pigs (13,22,24), but also in humans (8,19) and rats (2,10,11,12).…”

mentioning

confidence: 99%

Microdialysis Study of Imipenem Distribution in the Intraperitoneal Fluid of Rats with or without Experimental Peritonitis

Lefeuvre

Marchand

Lamarche

et al. 2006

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The purpose of this study was to extend the use of microdialysis to the investigation of antibiotic distribution into the intraperitoneal fluid of rats with or without peritonitis. Microdialysis probes were inserted into the jugular vein and peritoneal cavity of control rats or rats with intra-abdominal sepsis (n ‫؍‬ 8 in each group) induced by cecal ligation and punctures. Imipenem (IPM) probe recoveries were determined in each rat by retrodialysis by drug. IPM was infused intravenously at a dose of 30 mg · kg ؊1 over 30 min, microdialysis samples were collected for 120 min, and IPM concentrations were determined by high-performance liquid chromatography. Intraperitoneal infection had no statistically significant effect on IPM clearance (11.9 ؎ 2.3 ml · min ؊1 · kg ؊1 in control rats versus 10.9 ؎ 2.1 ml · min ؊1 · kg ؊1 in rats with peritonitis) or the volume of distribution (296 ؎ 47 ml · kg ؊1 in control rats versus 310 ؎ 49 ml · kg ؊1 in rats with peritonitis). IPM concentration profiles in intraperitoneal fluid and blood were virtually superimposed in control rats, whereas in infected animals, the mean intraperitoneal IPM concentrations were apparently slightly lower than corresponding blood levels. However, the areas under the concentration-versus-time curve estimated in intraperitoneal fluid and blood were not significantly different in both groups, with the corresponding ratios close to unity (1.01 ؎ 0.19 and 0.89 ؎ 0.28 in control rats and rats with peritonitis, respectively). In conclusion, IPM distribution in intraperitoneal fluid is rapid and complete both in control rats and in rats with peritonitis.

show abstract

“…Commercially available microdialysis probes with 100-kDa MWCO membranes have been reported for in vivo microdialysis of a few select cytokines [12][13][14]. The use of particle-based immunoassays has potential to be highly advantageous to the sample-limited microdialysis process since several analytes can be analyzed in a single low volume sample.…”

mentioning

confidence: 99%

Multiplexed cytokine detection in microliter microdialysis samples obtained from activated cultured macrophages

Wang

Lennartz

et al. 2006

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

Microdialysis: A New Technique to Monitor Perioperative Human Peritoneal Mediator Production

Abstract: The samples derived from microdialysis were suitable to measure sub-peritoneal mediator profiles during surgery and up to 7 days postoperatively. Microdialysis data should be validated for a potential correlation with the clinical course.

Cited by 15 publications

References 17 publications

Microdialysis for Monitoring Inflammation: Efficient Recovery of Cytokines and Anaphylotoxins Provided Optimal Catheter Pore Size and Fluid Velocity Conditions

Microdialysis for Monitoring Inflammation: Efficient Recovery of Cytokines and Anaphylotoxins Provided Optimal Catheter Pore Size and Fluid Velocity Conditions

Microdialysis Study of Imipenem Distribution in the Intraperitoneal Fluid of Rats with or without Experimental Peritonitis

Multiplexed cytokine detection in microliter microdialysis samples obtained from activated cultured macrophages

Contact Info

Product

Resources

About