Microdialysate analysis of monoamine neurotransmitters—A versatile and sensitive LC–MS/MS method

Greco, Sergio; Danysz, Wojciech; Živković, Aleksandra; Gross, Richard W.; Stark, Holger

doi:10.1016/j.aca.2013.02.004

Cited by 68 publications

(42 citation statements)

References 24 publications

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“…Some of these methods have lower LOQ for determination of NTs in rodent ECF and brain tissue than achieved in this study [16,18,20]. However, many of the methods for determination of NTs and metabolites include time consuming derivatization steps [14,18,19] or freeze drying [20], the use of ion pair chromatography which is unfavorable using MS [11,12,28] or relatively long analysis times (10-20 min) [7,[14][15][16][17].…”

Section: Comparison With Other Methodsmentioning

confidence: 79%

“…GABA, Glu and NE are the most polar NTs included in the methods and had restricted retention on the reversed phase columns for all conditions tested. The most satisfactory retention and resolution were obtained with the HSS T3 C 18 the analytes separated using the optimized parameters are shown in Fig. 2.…”

Section: Optimization Of Chromatographic Separation and Ms/ms Parametersmentioning

confidence: 87%

“…Previously reported methods for determination of NTs and NT metabolites have displayed long analysis times (10-19.5 min) [7,[14][15][16][17] or time consuming sample preparation including derivatization [14,18,19] or freeze drying [20]. However, methods employing derivatization and freeze drying have been reported to give advantages such as low limits of quantification [18,20].…”

Section: Introductionmentioning

confidence: 99%

“…However, methods employing derivatization and freeze drying have been reported to give advantages such as low limits of quantification [18,20]. The aim of the present study was to develop and validate fast and simple UHPLC-MS/MS methods for simultaneous determination of the NTs DA, 5-HT, NE, ACh, Glu, GABA and the DA metabolites 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 3-methoxytyramine (3-MT) and the 5-HT metabolite 5-hydroxyindoleacetic acid (5-HIAA) ( Fig.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Validated methods for determination of neurotransmitters and metabolites in rodent brain tissue and extracellular fluid by reversed phase UHPLC–MS/MS

Bergh

Bogen

Lundanes

et al. 2016

Journal of Chromatography B

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Section: Comparison With Other Methodsmentioning

confidence: 79%

Section: Optimization Of Chromatographic Separation and Ms/ms Parametersmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Validated methods for determination of neurotransmitters and metabolites in rodent brain tissue and extracellular fluid by reversed phase UHPLC–MS/MS

Bergh

Bogen

Lundanes

et al. 2016

Journal of Chromatography B

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“…A dialysis cannula is stereotaxically implanted into the brain and samples are collected for identification by high-performance liquid chromatography and electrochemical detection. While microdialysis can accurately identify neurotransmitters 9,10 , it requires the collection of relatively large samples and has poor temporal resolution, exceeding ten minutes per sample in the case of monoamines 11 . Moreover, the insertion of microdialysis probes can disrupt monoaminergic activity near the probe track 12 .…”

Section: Introductionmentioning

confidence: 99%

Cell-based reporters reveal in vivo dynamics of dopamine and norepinephrine release in murine cortex

Muller¹,

Joseph²,

Slesinger³

et al. 2014

Nat Methods

130

145

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Neuronal coding of stimulus-to-action sequences are believed to involve the release of dopamine (DA) and norepinephrine (NE). The electrochemical similarity of these monoamines, however, confounds real-time measurements of their release. Here we report the creation of cell-based neurotransmitter fluorescent-engineered reporters (CNiFERs) that utilize the specificity of G-protein coupled receptors (GPCRs) to discriminate nanomolar concentrations of DA and NE. CNiFERs were implanted into frontal cortex of mice to measure the timing of neurotransmitter release during classical conditioning using two-photon microscopy. The onset of DA release correlated with that of licking and monotonically shifted from the time of the reward toward that of the cue. In contrast, concurrent release of NE did not correlate with licking or the cue. This new generation of CNiFERs provides unique tools to assess the release of monoamines. The molecular design of these CNiFERs may be generalized to realize CNiFERs for any molecule that activates a GPCR.

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LC/MS/MS analysis of the endogenous dimethyltryptamine hallucinogens, their precursors, and major metabolites in rat pineal gland microdialysate

Barker

Borjigin

Lomnicka

et al. 2013

Biomedical Chromatography

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We report a qualitative liquid chromatography-tandem mass spectrometry (LC/MS/MS) method for the simultaneous analysis of the three known N,N-dimethyltryptamine endogenous hallucinogens, their precursors and metabolites, as well as melatonin and its metabolic precursors. The method was characterized using artificial cerebrospinal fluid (aCSF) as the matrix and was subsequently applied to the analysis of rat brain pineal gland-aCSF microdialysate. The method describes the simultaneous analysis of 23 chemically diverse compounds plus a deuterated internal standard by direct injection, requiring no dilution or extraction of the samples. The results demonstrate that this is a simple, sensitive, specific and direct approach to the qualitative analysis of these compounds in this matrix. The protocol also employs stringent MS confirmatory criteria for the detection and confirmation of the compounds examined, including exact mass measurements. The excellent limits of detection and broad scope make it a valuable research tool for examining the endogenous hallucinogen pathways in the central nervous system. We report here, for the first time, the presence of N,N-dimethyltryptamine in pineal gland microdialysate obtained from the rat.

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Microdialysate analysis of monoamine neurotransmitters—A versatile and sensitive LC–MS/MS method

Cited by 68 publications

References 24 publications

Validated methods for determination of neurotransmitters and metabolites in rodent brain tissue and extracellular fluid by reversed phase UHPLC–MS/MS

Validated methods for determination of neurotransmitters and metabolites in rodent brain tissue and extracellular fluid by reversed phase UHPLC–MS/MS

Cell-based reporters reveal in vivo dynamics of dopamine and norepinephrine release in murine cortex

LC/MS/MS analysis of the endogenous dimethyltryptamine hallucinogens, their precursors, and major metabolites in rat pineal gland microdialysate

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