Microcystin-LR-induced cytotoxicity and apoptosis in human embryonic kidney and human kidney adenocarcinoma cell lines

Piyathilaka, M.A.P.C.; Manage, P.M.; Tennekoon, Kamani Hemamala; Silva, B. G. D. N. K. De; Samarakoon, Sameera Ranganath; Chanthirika, S.

doi:10.1099/mic.0.000046

Cited by 42 publications

(35 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Yatigammana et al, 2011), though it was caused by a dinoflagellate, Peridinium aciculiferum. Piyathilaka et al (2015) recorded that Microcystin-LR-induced cytotoxicity and apoptosis in human embryonic kidney and human kidney adenocarcinoma cell lines.…”

Section: Discussionmentioning

confidence: 99%

Distribution of <em>Cylindrospermopsis raciborskii</em> (Cyanobacteria) in Sri Lanka

Yatigammana

Perera

2017

Ceylon J. Sci.

View full text Add to dashboard Cite

Sri Lanka is a tropical continental island which consists of 103 natural rivers and over ten thousand man-made lakes. Majority of these water resources are known to be contaminated with different types of toxigenic cyanobacteria making water unsuitable for human and animal consumption. Cylindrospermopsis raciborskii, a toxin producing tropical cyanobacterium, recently recorded at high abundances in lentic waters of the country. This species is highly adaptive and exhibit different morphotypes: straight, coiled and sigmoid-shaped trichomes under different environmental conditions. Distribution and abundance of the species is mainly depending on the environmental factors and therefore remedies can be proposed to keep the populations under control. This preliminary study was conducted to understand the prevalence of C. raciborskii, in Sri Lankan reservoirs. Sixty three reservoirs representing all climatic regions with varying environmental conditions were assessed using standard procedures. The results of the study reviewed that C. raciborskii is a dominant cyanobacterium species especially during the dry season in shallow urban reservoirs, terminal reservoirs in large cascades, reservoirs with low flushing rates and deep reservoirs with persistent water stratification. Morphological variations were common in urban and shallow reservoirs that maintain high water retention time. They commonly occur in coiled-shape. However, in eutrophic water bodies they are appeared to support other morphotypes: straight, sigmoid or tricomatous. Further, it was noticed that the cell size of C. raciborskii is exceptionally large (width 3.5 µm and length 7µm) in a high altitudinal wet-zone reservoir that usually maintains temperature range of 10 o C -20 o C throughout the year. The results showed that C. raciborskii is widely distributed all over the country and the presence of several morphotypes signifies its potential for rapid distribution.

show abstract

Section: Discussionmentioning

confidence: 99%

Distribution of <em>Cylindrospermopsis raciborskii</em> (Cyanobacteria) in Sri Lanka

Yatigammana

Perera

2017

Ceylon J. Sci.

View full text Add to dashboard Cite

show abstract

“…Several studies revealed that MCs may produce nephrotoxicity and generate cancer-promoting effect. In cells, exposure to MC-LR could induce apoptosis in both human embryonic kidney (HEK-293) and human kidney adenocarcinoma (ACHN) cell lines by reducing G2/M phase population [20]. In aquatic organisms, renal lesions were observed.…”

Section: Introductionmentioning

confidence: 99%

Effects of Chronic Exposure to Microcystin-LR on Kidney in Mice

Huang

et al. 2019

IJERPH

View full text Add to dashboard Cite

Microcystin-LR (MC-LR) is a potent hepatotoxin, but a few studies suggested that it might also induce nephrotoxicity. However, nephrotoxicity induced by prolonged oral exposure to MC-LR is unknown. The aim of this study was to evaluate the potential influence of MC-LR on the kidney in mice following chronic exposure to MC-LR. In this study, we evaluated the nephrotoxicity of MC-LR in mice drinking water at different concentrations (1, 30, 60, 90, and 120 µg/L) for 6 months for the first time. The results showed that the kidney weights and the kidney indexes of mice were not altered in the MC-LR treated mice, compared with the control group. In addition, the renal function indicators revealed that the serum creatinine (SCr) levels were not significant changes after exposure to MC-LR. The blood urea nitrogen (BUN) levels were markedly decreased after exposure to 90 and 120 µg/L MC-LR for 3 months. The BUN levels were lower than that of the control group after exposure to 120 µg/L MC-LR for 6 months. The histopathological investigation revealed enlarged renal corpuscles, widened of kidney tubules, and lymphocyte infiltration in the interstitial tissue and the renal pelvis after exposure to 60, 90, and 120 µg/L MC-LR. Consequently, our results suggested that long-term exposure to MC-LR might be one important risk of kidney injury, which will provide important clues for the prevention of renal impairment.

show abstract

“…Microcystin-LR (MC-LR) is the most toxic cyanotoxin variant most commonly encountered in contaminated aquatic systems (Jones and Orr, 1994). MC-LR is a potent hepatotoxin, but increasing evidences suggest that it might also induce kidney injury (Piyathilaka et al, 2015). There is an increasing trend in mortality and morbidity due to chronic kidney disease (CKD) in Sri Lanka where the underlying cause of CKD remains complicated.…”

Section: Introductionmentioning

confidence: 99%

Microcystin-LR Contamination Status and Physico-Chemical Water Quality Parameters of Five Selected Recreational Water Bodies in Sri Lanka

Piyathilaka

Manage

2017

J Food & Agric

View full text Add to dashboard Cite

Microcystin-LR (MC-LR) is the most toxic and commonly encountered cyanotoxin variant in aquatic systems. MC-LR is a hepatotoxin, but evidences suggest that it might also induce kidney injury. There is an increasing trend in mortality due to chronic kidney disease (CKD) in Sri Lanka where the underlying cause of CKD remains complicated. Cyanobacterial toxins is considered as one of the possible reasons implicated in the aetiology of CKDu in Sri Lanka.A significant source of cyanobacterial toxin exposure is recreational use of contaminated freshwater bodies. Therefore, it was a timely need to assess MC-LR contamination status. Thus, in the present study, freshwater bodies of Beira, Boralesgamuwa, Kandy, Kurunegala and Hot water springs in Kinniya were sampled. Triplicate surface water samples were collected from each water body. Surface water temperature, pH, conductivity and dissolved oxygen (DO) were measured at the site itself and chemical analyses of nitrate, total phosphate and chlorophyll-a were carried out.water bodies showed MC-LR contamination. The concentrations of MC-LR from the highest to the lowest were reported respectively from Beira lake (2198 ppb), Kandy lake (103 ppb), Kurunegala lake (75.6 ppb) and the Hot water springs (0.34 ppb). Results of the present study revealed that pH, nitrate, total phosphate and chlorophyll-a contents showed significant positive correlation with the MC-LR concentration of each water body (p<0.05) while surface water temperature, DO and conductivity did not show significant correlation to MC-LR contamination.

show abstract

Microcystin-LR-induced cytotoxicity and apoptosis in human embryonic kidney and human kidney adenocarcinoma cell lines

Cited by 42 publications

References 44 publications

Distribution of <em>Cylindrospermopsis raciborskii</em> (Cyanobacteria) in Sri Lanka

Distribution of <em>Cylindrospermopsis raciborskii</em> (Cyanobacteria) in Sri Lanka

Effects of Chronic Exposure to Microcystin-LR on Kidney in Mice

Microcystin-LR Contamination Status and Physico-Chemical Water Quality Parameters of Five Selected Recreational Water Bodies in Sri Lanka

Contact Info

Product

Resources

About