Microcystin-LR heterologous genetically engineered antibody recombinant and its binding activity improvement and application in immunoassay

Xu, Chongxin; He, Duofen; Zu, Yao; Hong, Sujuan; Hao, Jia; Li, Jianhong

doi:10.1016/j.jhazmat.2020.124596

Cited by 16 publications

(6 citation statements)

References 60 publications

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“…101 Subsequently, Xu et al 99 successfully isolated Nb antibodies against MC-LR and employed targeted mutagenesis to generate evolved Nbs for detection purposes. 102 Nevertheless, despite the preponderance of MC detection occurring in vitro, 103 further research is imperative to propel the field of in vivo neutralization forward. Apart from this, nodularins (NODs) are harmful cyanobacteria toxins produced by Nodularia spumigena.…”

Section: Nbs For Phytotoxin Neutralizationmentioning

confidence: 99%

“…The selection process involved the utilization of competitive panning alongside two innovative panning strategies . Subsequently, Xu et al successfully isolated Nb antibodies against MC-LR and employed targeted mutagenesis to generate evolved Nbs for detection purposes . Nevertheless, despite the preponderance of MC detection occurring in vitro , further research is imperative to propel the field of in vivo neutralization forward.…”

Section: Progress Of Nbs Against Food-borne Biotoxins and The Neutral...mentioning

confidence: 99%

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Food-Borne Biotoxin Neutralization in Vivo by Nanobodies: Current Status and Prospects

Liu,

Liang,

Jin

et al. 2024

J. Agric. Food Chem.

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Food-borne biotoxins from microbes, plants, or animals contaminate unclean, spoiled, and rotten foods, posing significant health risks. Neutralizing such toxins is vital for human health, especially after food poisoning. Nanobodies (Nbs), a type of single-domain antibodies derived from the genetic cloning of a variable domain of heavy chain antibodies (VHHs) in camels, offer unique advantages in toxin neutralization. Their small size, high stability, and precise binding enable effective neutralization. The use of Nbs in neutralizing food-borne biotoxins offers numerous benefits, and their genetic malleability allows tailored optimization for diverse toxins. As nanotechnology continues to evolve and improve, Nbs are poised to become increasingly efficient and safer tools for toxin neutralization, playing a pivotal role in safeguarding human health and environmental safety. This review not only highlights the efficacy of these agents in neutralizing toxins but also proposes innovative solutions to address their current challenges. It lays a solid foundation for their further development in this crucial field and propels their commercial application, thereby contributing significantly to advancements in this domain.

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Section: Nbs For Phytotoxin Neutralizationmentioning

confidence: 99%

Section: Progress Of Nbs Against Food-borne Biotoxins and The Neutral...mentioning

confidence: 99%

Food-Borne Biotoxin Neutralization in Vivo by Nanobodies: Current Status and Prospects

Liu,

Liang,

Jin

et al. 2024

J. Agric. Food Chem.

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“…Su et al 148 Microcystin-LR (MC-LR) is a kind of highly toxic biohazard produced by several bloom-forming cyanobacteria that seriously pollute the ecological environment and agro-products. Xu et al 149 designed a newly recombinant genetically engineered antibody (AV HH -MV HH ) based on the previously obtained anti-MC-LR single-chain antibody and nanobody to establish an indirect competitive ELISA (IC-ELISA), with the LOD of 0.0075 μg/L, which is far below the maximum residue limit standard of 1.0 μg/L for MC-LR in drinking water proposed by WHO, showing good accuracy, repeatability, stability, applicability, and ultrasensitive binding activity. The nanobodies employed for the detection of some small molecules are listed in Table 3.…”

Section: Nanobody As a Reagent In Toxin Detectionmentioning

confidence: 99%

Recent advances of nanobody applications in diagnosis and detection

Su,

Shi,

Huang

et al. 2023

MedComm – Biomaterials and Applications

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Nanobodies (Nbs) are the variable domain of heavy‐chain antibodies derived from the blood of camelids or sharks. Nanobodies are the smallest antibody fragment with intact antigen‐binding ability. Compared to conventional antibodies, nanobodies have unique properties such as small size, excellent stability and solubility, low immunogenicity, ability to recognize hidden epitopes, high tissue penetration, and industrialized production. More excitingly, the camelid‐specific amino acid sequences in the framework are mutated to their human heavy‐chain variable domain equivalent, which is humanized, to a wide range of applications. These superior characteristics make nanobody an ideal alternative to conventional antibody, showing excellent prospects for various applications in structural biology, molecular imaging, disease diagnosis and therapy, agricultural products, and environmental chemicals detection. With the continuous updating of theories and the rapid development of technology, the screening and expression methods of nanobodies are increasingly mature. Consequently, several technologies to identify and express nanobodies have been established, and various use cases have been described. In this review, we summarize recent advances in the discovery and production of novel nanobodies, and their use in detection and diagnosis platforms.

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“…Note that the MC-LR levels in all three water types were lower than the limit of 1.0 μg/L in water supply proposed by the World Health Organization. 29…”

Section: Dovepressmentioning

confidence: 99%

Genetic Variant of PP2A Subunit Gene Confers an Increased Risk of Primary Liver Cancer in Chinese

Wang¹,

Huang²,

Huang³

et al. 2021

PGPM

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Background: Protein phosphatase 2A (PP2A, a serine/threonine phosphatase) is frequently inactivated in many types of cancer, including primary liver cancer (PLC). Genetic variations in PP2A subunits have been reported to be associated with the risk of many types of cancer but rarely in PLC. This study aims to assess the association between functional polymorphisms of PP2A subunit genes and the risk of PLC in Chinese. Methods: In a case-control study with a total of 541 PLC patients and 547 controls in Guangxi province of Southern China, we genotyped six putatively functional polymorphisms (rs10421191G>A, rs11453459del>insG, rs1560092T>G, rs7840855C>T, rs1255722G>A and rs10151527A>C) of three PP2A subunit genes (PPP2R1A, PPP2R2A and PPP2R5E) using matrixassisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry platform. Results: The rs11453459insG variant genotypes (ins/ins+del/ins) of PPP2R1A were found to be significantly associated with an increased risk of PLC compared with the del/del genotype (adjusted OR = 1.290, 95% CI = 1.009-1.650), and the number of insert G allele worked in a dose-dependent manner (P trend = 0.007). The stratified analysis showed that the effects of rs11453459insG variant genotypes were more evident in the subgroup who drink pond-ditch water (adjusted OR = 3.051, 95% CI = 1.264-7.364) than those never drink (P = 0.041). The carriers of rs11453459 del/ins genotype had a significantly lower level of PPP2R1A mRNA expression in liver cancer tissues than those of the del/del genotype (P = 0.021). Furthermore, we used microcystin-LR, a carcinogen presents in the pond-ditch water, to treat human peripheral blood mononuclear cells and found that the cells from carriers of rs11453459insG variant genotypes induced more DNA oxidative damages than those from the del/del genotype carriers (P < 0.001). Conclusion: These findings suggest that the PPP2R1A rs11453459del>insG polymorphism is associated with an increased risk of PLC, especially for persons with a history of drinking pond-ditch water. This insertion/deletion polymorphism may be a susceptible biomarker for PLC in Chinese.

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Microcystin-LR heterologous genetically engineered antibody recombinant and its binding activity improvement and application in immunoassay

Cited by 16 publications

References 60 publications

Food-Borne Biotoxin Neutralization in Vivo by Nanobodies: Current Status and Prospects

Food-Borne Biotoxin Neutralization in Vivo by Nanobodies: Current Status and Prospects

Recent advances of nanobody applications in diagnosis and detection

Genetic Variant of PP2A Subunit Gene Confers an Increased Risk of Primary Liver Cancer in Chinese

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