Microcycle conidiation and its genetic basis in Neurospora crassa

Maheshwari, Ramesh

doi:10.1099/00221287-137-9-2103

Cited by 39 publications

(27 citation statements)

References 44 publications

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“…Some of these studies defined newly discovered loci. New examples are cdr (Levine and Marzluf, 1989), mcb and mcm (Maheshwari, 1991), and sen (Navaraj et al, 2000). Other studies involved new alleles at already established loci, for example, a novel nmr allele (Young and Marzluf, 1991).…”

Section: Other Laboratory Investigations Using Naturally Occurring Vamentioning

confidence: 97%

Neurospora from Natural Populations: A Global Study

Turner

Perkins

Fairfield

2001

Fungal Genetics and Biology

124

148

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Section: Other Laboratory Investigations Using Naturally Occurring Vamentioning

confidence: 97%

Neurospora from Natural Populations: A Global Study

Turner

Perkins

Fairfield

2001

Fungal Genetics and Biology

124

148

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“…In most species studied, microcycle conidiation is induced by physical and chemical stresses (80). Thus, this process may provide a significant survival advantage (77,81,82).…”

Section: Figmentioning

confidence: 99%

WetA Is Required for Conidiogenesis and Conidium Maturation in the Ascomycete Fungus Fusarium graminearum

et al. 2014

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c Fusarium graminearum, a prominent fungal pathogen that infects major cereal crops, primarily utilizes asexual spores to spread disease. To understand the molecular mechanisms underlying conidiogenesis in F. graminearum, we functionally characterized the F. graminearum ortholog of Aspergillus nidulans wetA, which has been shown to be involved in conidiogenesis and conidium maturation. Deletion of F. graminearum wetA did not alter mycelial growth, sexual development, or virulence, but the wetA deletion mutants produced longer conidia with fewer septa, and the conidia were sensitive to acute stresses, such as oxidative stress and heat stress. Furthermore, the survival rate of aged conidia from the F. graminearum wetA deletion mutants was reduced. The wetA deletion resulted in vigorous generation of single-celled conidia through autophagy-dependent microcycle conidiation, indicating that WetA functions to maintain conidial dormancy by suppressing microcycle conidiation in F. graminearum. Transcriptome analyses demonstrated that most of the putative conidiation-related genes are expressed constitutively and that only a few genes are specifically involved in F. graminearum conidiogenesis. The conserved and distinct roles identified for WetA in F. graminearum provide new insights into the genetics of conidiation in filamentous fungi.

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“…Since then attempts have been made to induce this phenomenon in other Aspergillm spp. and fungi by manipulating culture conditions and/or nutrient factors (Maheshwari, 1991). Earlier, we reported MC on a novel medium, D-glucose soluble starch (D-GSS) in Aspergillus spp.…”

Section: Introductionmentioning

confidence: 99%

Polyamines as Modulators of Microcycle Conidiation in Aspergillus Flavus

et al. 1996

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Since polyamines (PAS) play a potential role in the regulation of growth and developmental processes in a wide variety of organisms, we have examined the influence of the PAS putrescine (Put) and spermidine (Spd) and the PA biosynthetic inhibitors a-difluoromethylornithine (DFMO), adif luoromethylarginine (DFMA), methylglyoxal bis-(guanylhydrazone) (MGBG) and cyclohexylamine (CHA), singly and in combinations on microcycle conidiation (MC) in Aspergillus flaws. The exogenous application of the diamine Put (concentrations ranging from 01 to 5 mM) caused a sharp decline of MC in a dose-dependent fashion, but induced vegetative growth. However, the triamine Spd (01-5 mM) had a minimal effect on M C and induced a shift from M C to normal conidiation. PA inhibitors, especially DFMO, MGBG and CHA, produced greater inhibition of MC and complete inhibition of M C was observed at 5 mM of these inhibitors. DFMA even at 5 mM had only a weak inhibitory effect on MC. DFMO also inhibited conidial germination and germ tube growth. MGBG and CHA, while having an inhibitory effect on MC, induced vegetative growth. The inhibitory effect of PA inhibitors was partially reversed by exogenous Put or Spd, with Spd being more effective than Put. The analysis of free PA levels during various phases of M C revealed that undifferentiated spores contained a high Put/Spd ratio and there was a dramatic decrease in Put/Spd ratio before and during microcycle conidiophore maturity. The change in spermine titres could not be detected. These observations imply that Put is essential for vegetative growth, while S p d is involved in MC, and that a low Put/Spd ratio seems to be important for spore differentiation to MC.

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Microcycle conidiation and its genetic basis in Neurospora crassa

Cited by 39 publications

References 44 publications

Neurospora from Natural Populations: A Global Study

Neurospora from Natural Populations: A Global Study

WetA Is Required for Conidiogenesis and Conidium Maturation in the Ascomycete Fungus Fusarium graminearum

Polyamines as Modulators of Microcycle Conidiation in Aspergillus Flavus

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