Microconfined flow behavior of red blood cells

Tomaiuolo, Giovanna; Lanotte, Luca; D’Apolito, Rosa; Cassinese, A.; Guido, Stefano

doi:10.1016/j.medengphy.2015.05.007

Cited by 55 publications

(40 citation statements)

References 37 publications

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“…Moreover, Quint et al [26] found a stable slipper and a metastable croissant at the same set of parameters in a wider channel of 25 µm × 10 µm. Other publications presenting experiments in channel flow also touch the subject of RBC shapes but focus on other aspects such as the methodology [27][28][29][30][31][32][33][34], dense suspensions and cell interactions [17,21,[34][35][36][37][38][39][40][41] or use vastly larger channel diameters [12,42].…”

Section: Introductionmentioning

confidence: 99%

Numerical–experimental observation of shape bistability of red blood cells flowing in a microchannel

et al. 2018

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Red blood cells flowing through capillaries assume a wide variety of different shapes owing to their high deformability. Predicting the realized shapes is a complex field as they are determined by the intricate interplay between the flow conditions and the membrane mechanics. In this work we construct the shape phase diagram of a single red blood cell with a physiological viscosity ratio flowing in a microchannel. We use both experimental in vitro measurements as well as 3D numerical simulations to complement the respective other one. Numerically, we have easy control over the initial starting configuration and natural access to the full 3D shape. With this information we obtain the phase diagram as a function of initial position, starting shape and cell velocity. Experimentally, we measure the occurrence frequency of the different shapes as a function of the cell velocity to construct the experimental diagram which is in good agreement with the numerical observations. Two different major shapes are found, namely croissants and slippers. Notably, both shapes show coexistence at low (<1 mm s) and high velocities (>3 mm s) while in-between only croissants are stable. This pronounced bistability indicates that RBC shapes are not only determined by system parameters such as flow velocity or channel size, but also strongly depend on the initial conditions.

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Section: Introductionmentioning

confidence: 99%

Numerical–experimental observation of shape bistability of red blood cells flowing in a microchannel

et al. 2018

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“…Erythrocyte deformability is a key factor of microcirculation [9]. We have investigated this deformability as a filterability of erythrocytes passing through the narrow pores distributed regularly on the nickel mesh filter ( Figure 3A).…”

Section: Discussionmentioning

confidence: 99%

Assessment of erythrocyte deformability in an obese case of chronic thromboembolic pulmonary hypertension

et al. 2017

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Erythrocyte deformability plays a key role in pulmonary microcirculation, which raised the hypothesis that erythrocyte deformability is impaired in pulmonary thromboembolism (PTE) and subsequent chronic thromboembolic pulmonary hypertension (CTEPH). We encountered a case of PTE followed by CTEPH and investigated erythrocyte deformability by our specified filtration technique to test this hypothesis. Erythrocyte deformability was normal before but was impaired after the onset of PTE. It was restored partially in the stage of CTEPH. This case taught us that erythrocyte deformability is impaired and that this impairment relates to the hemodynamics of pulmonary microcirculation and pathophysiology of PTE and CTEPH.

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“…For instance, at Ca = 0.25, an RBC is in the tumbling regime Sinha and Graham (2015) and therefore the migration is slower, whereas for Ca≥ 0.5, the RBC is in the tank-treading regime and lifts more quickly (Qi and Shaqfeh, 2017;Saadat et al, 2018). Slipper-like and parachute-like shapes for RBCs have been extensively reported in the literature Kaoui et al (2009) ;Quint et al (2017); Fedosov et al (2014); Tomaiuolo et al (2016); Guckenberger et al (2018). However, experiments of Lanotte et.…”

Section: Introductionmentioning

confidence: 99%

Effect of Cytoplasmic Viscosity on Red Blood Cell Migration in Small Arteriole-level Confinements

Saadat

Guido

Shaqfeh³

2019

Preprint

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The dynamics of red blood cells in small arterioles are important as these dynamics affect many physiological processes such as hemostasis and thrombosis. However, studying red blood cell flows theoretically is challenging due to the complex shapes of red blood cells and the non-trivial viscosity contrast of a red blood cell. To date little progress has been made studying small arteriole flows (20-40µm) with a hematocrit (red blood cell volume fraction) of 10-20% and a physiological viscosity contrast. In this work, we present the results of large-scale simulations that show how the channel size, viscosity contrast of the red blood cells, and hematocrit effect cell distributions and the cell free layer in these systems. We utilize a massively-parallel immersed boundary code coupled to a finite volume solver to capture particle resolved physics in these systems. We show that channel size qualitatively changes how the cells distribute in the channel. Our results also indicate that at a hematocrit of 10% that the viscosity contrast is not negligible when calculating the cell free layer thickness. We explain this result by comparing lift and collision trajectories of cells at different viscosity contrasts.

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Microconfined flow behavior of red blood cells

Cited by 55 publications

References 37 publications

Numerical–experimental observation of shape bistability of red blood cells flowing in a microchannel

Numerical–experimental observation of shape bistability of red blood cells flowing in a microchannel

Assessment of erythrocyte deformability in an obese case of chronic thromboembolic pulmonary hypertension

Effect of Cytoplasmic Viscosity on Red Blood Cell Migration in Small Arteriole-level Confinements

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