Microcomputed Tomography to Visualize and Quantify Fungal Infection Burden and Inflammation in the Mouse Lung Over Time

Vanhoffelen, Eliane; Reséndiz-Sharpe, Agustin; Velde, Greetje Vande

doi:10.1007/978-1-0716-3199-7_16

Cited by 1 publication

(1 citation statement)

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“…Taken together, it is crucial for the ITI to find the right inoculum concentration that balances between clearance and premature death of the animal [191]. Careful and strain-specific optimization is needed for this model in mice and rats [191,198]. A second hurdle when using this technique is the variable disease outcome and lower level of reproducibility.…”

Section: Intratracheal and Intranasal Installationmentioning

confidence: 99%

Preclinical Models for Cryptococcosis of the CNS and Their Characterization Using In Vivo Imaging Techniques

Roosen,

Maes,

Musetta

et al. 2024

JoF

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Infections caused by Cryptococcus neoformans and Cryptococcus gattii remain a challenge to our healthcare systems as they are still difficult to treat. In order to improve treatment success, in particular for infections that have disseminated to the central nervous system, a better understanding of the disease is needed, addressing questions like how it evolves from a pulmonary to a brain disease and how novel treatment approaches can be developed and validated. This requires not only clinical research and research on the microorganisms in a laboratory environment but also preclinical models in order to study cryptococci in the host. We provide an overview of available preclinical models, with particular emphasis on models of cryptococcosis in rodents. In order to further improve the characterization of rodent models, in particular the dynamic aspects of disease manifestation, development, and ultimate treatment, preclinical in vivo imaging methods are increasingly used, mainly in research for oncological, neurological, and cardiac diseases. In vivo imaging applications for fungal infections are rather sparse. A second aspect of this review is how research on models of cryptococcosis can benefit from in vivo imaging methods that not only provide information on morphology and tissue structure but also on function, metabolism, and cellular properties in a non-invasive way.

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Section: Intratracheal and Intranasal Installationmentioning

confidence: 99%