Microclonal propagation in vitro of commercial varieties of rose

Tolembetova, А. K.; Turasheva, S. K.; Imanbaeva, A. A.; Ernazarova, G. I.; Serikova, Z. B.

doi:10.26577/eb-2017-4-1300

Cited by 2 publications

(1 citation statement)

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“…Clonal micropropagation is one of the most effective ways to obtain plant material in significant volumes in a short period [3,4]. Micropropagation technologies are known and well-developed, but their improving is necessary for efficiency increase [5]. At the same time, the use of in vitro technology, in particular clonal micropropagation, is associated with several problems, which in turn affect the experiment's efficiency and productivity.…”

Section: Introductionmentioning

confidence: 99%

In vitro introduction of Prunus persica (L.) Batsch ‘Dostoyniy’

Bilyak,

Sidyakin,

Bulavin

et al. 2023

E3S Web Conf.

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The article discusses the introduction in vitro, selection of the nutrient medium and plant growth regulator effect on the microshoot development of the perspective fruit crop Prunus persica ‘Dostoyniy’. Murashige and Skoog (MS), Gamborg and Eveleg (B5), Blaydes (BL), McCown (WPM) media supplemented with 0.5 mg/l 6-BAP, 0.1 mg/l IBA; 1.0 mg/l 6-BAP, 0.025 mg/l IBA; 0.48 mg/l KIN, 0.1 mg/l IBA; 0.96 mg/l KIN, 0.025 mg/l IBA and succinic acid were used in our research. According to obtained results, the most optimal season for the introduction of P. persica ‘Dostoiniy’ into in vitro culture was summer. The use of alcohol-, and chlorine-containing sterilizing agents in combination with antibiotics (or without them) provides aseptic material. BL and B5 media supplemented with 0.5 mg/l 6-BAP and 0.1 mg/l IBA, as well as 1.0 mg/l 6-BAP and 0.025 mg/l IBA promote microshoot development in vitro. Succinic acid in the culture medium at concentration of 5 and 10 g/l contributes to an additional increase in the multiplication rate.

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Section: Introductionmentioning

confidence: 99%

In vitro introduction of Prunus persica (L.) Batsch ‘Dostoyniy’

Bilyak,

Sidyakin,

Bulavin

et al. 2023

E3S Web Conf.

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Propagation of Canadian rose ‘John Franklin’ in vitro culture

Zaripova,

Akhmetova,

Shigapova

2024

BIO Web Conf.

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Many researchers are interested in optimizing the clonal micropropagation of ornamental plants. It is known that the varietal specificity of the studied crops makes it necessary to select and optimize the composition of the nutrient medium for each stage of propagation in vitro. The purpose of our research was to identify the effect of the composition and concentration of nutrient hormones on multiple shoot formation, root development in regenerating plants of the rose 'John Franklin' in vitro. A technology has been developed for obtaining a large amount of self-rooted planting material of a winter-hardy decorative rose variety in vitro culture. Clonal micropropagation was performed using vegetative buds as explants. The multiple of shoots is achieved by activating the axillary meristems of the shoots. It has been shown that the efficiency of micropropagation of the 'John Franklin' rose increases on the Murashige and Skoog nutrient medium containing BAP at a concentration of 1.0 mg/l and IAA – 0.5 mg/l. This medium provides high quality regeneration of micro-shoots with a propagation coefficient equal to 5.2. The optimal modification of the nutrient medium at the rooting stage was revealed, and the expediency of using IBA with a concentration of 0.5 mg/l as auxin was noted. The selected conditions allow you to get a larger number of roots on the shoot – 5 pcs. and their greatest length is 51.5 mm.

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Microclonal propagation in vitro of commercial varieties of rose

Cited by 2 publications

References 0 publications

In vitro introduction of Prunus persica (L.) Batsch ‘Dostoyniy’

In vitro introduction of Prunus persica (L.) Batsch ‘Dostoyniy’

Propagation of Canadian rose ‘John Franklin’ in vitro culture

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