Microcirculatory effects of prostaglandins E1, E2, and A1 in the rat mesentery and cremaster muscle

Messina, Edward J.; Weiner, Richard; Kaley, Gabor

doi:10.1016/0026-2862(74)90066-1

Cited by 51 publications

(16 citation statements)

References 20 publications

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“…A second major function regulated or modulated by products of the AA cascade is that of vascular reactivity. PGI2 and PGE2 can directly increase arteriolar diameter, while inhibition of PG synthesis can lead to reduced skeletal muscle vasodilitation induced by exercise or ischemia (51)(52)(53). PGE2, but not PGI2, can reduce the vasoconstrictor response to norepinephrine in the cremaster microcirculation (54,55).…”

Section: Resultsmentioning

confidence: 99%

Arachidonic acid and prostaglandin endoperoxide metabolism in isolated rabbit and coronary microvessels and isolated and cultivated coronary microvessel endothelial cells.

Gerritsen¹,

Cheli²

1983

J. Clin. Invest.

149

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A B S T R A C T Isolated microvessels and isolated and cultured microvessel endothelial cells were prepared from rabbit cardiac muscle. Pathways of arachidonic acid metabolism were determined by measurement of exogenous substrate utilization ([1-'4C]arachidonic acid incorporation and release from intact tissue and cells;[1-'4C]prostaglandin H2 (PGH2) metabolism by broken cell preparations) and by quantification of endogenous products (immunoreactive 6-keto-prostaglandin Fla (PGFia,) and prostaglandin E (PGE) release) by selective radioimmunoassay. Rabbit coronary microvessels and derived microvascular endothelial cells (RCME cells) synthesized two major products of the cyclooxygenase pathway: 6-keto-PGFI,, (hydrolytic product of prostaglandin 12) and PGE2. A reduced glutathione requiring PGH-E isomerase was demonstrated in coronary microvessels and RCME cells, but not in rabbit circumflex coronary artery or aorta. In addition, a minor amount of a compound exhibiting similar characteristics to 6-keto-PGE, was found to be produced by microvessels and RCME cells. Measurement of endogenously released prostaglandins indicated that under basal and stimulated conditions, PGE release ex-

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Section: Resultsmentioning

confidence: 99%

Arachidonic acid and prostaglandin endoperoxide metabolism in isolated rabbit and coronary microvessels and isolated and cultivated coronary microvessel endothelial cells.

Gerritsen¹,

Cheli²

1983

J. Clin. Invest.

149

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“…Corticosteroids may suppress the u.v.B response by inhibiting the inducible form of cyclo-oxygenase (Masferrer et al, 1992), by inhibiting phospholipase A2 (Axelrod, 1983), as well as by preventing the induction of iNOS. Prostaglandins are potent vasodilators of the microcirculation (Messina et al, 1975;Williams, 1979;Messina & Kaley, 1980), in contrast to their low activity in large blood vessels (Gordon & Martin, 1983;Gryglewski et al, 1986). Prostaglandins and nitric oxide might be synergistic in the microcirculation, analogous to their combined inhibition of platelet aggregation (Moncada & Higgs, 1991), although there is no evidence this is the case in the vasculature.…”

Section: Discussionmentioning

confidence: 99%

Involvement of nitric oxide synthase in the delayed vasodilator response to ultraviolet light irradiation of rat skin in vivo

Warren

Loi

Coughlan

1993

British J Pharmacology

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1 The role of nitric oxide synthase and cyclo-oxygenase in the skin blood flow response to ultraviolet light B (u.v.B) irradiation was investigated in the rat in vivo. 2 Local skin blood flow changes were measured in the shaved dorsal skin of anaesthetized male Sprague-Dawley rats with a laser Doppler flow probe. 3 u.v.B irradiation caused delayed onset vasodilatation and by 18 h basal blood flow increased by 125 ± 25% (P < 0.05, n = 12 rats, mean ± s.e.mean). 4 Indomethacin, 3 nmol per site, N0-nitro-L-arginine methyl ester (L-NAME) 100 nmol per site, but not D-NAME 100 nmol per site, injected locally 17.5 h after u.v.B irradiation abolished the 18 h increase in blood flow. 5 The nitric oxide synthase inhibitor L-NAME, NC-monomethyl-L-arginine (L-NMMA) and canavanine, 10 and 100 nmol per site injected at 17.5 h, suppressed significantly the u.v.B 18 h response in a dose-dependent manner. The order of potency was L-NAME> canavanine = L-NMMA. The effect of L-NAME was reversed partially by the co-injection of an excess of L-arginine. 6 Topical application of the corticosteroid, clobetasol 17-propionate, immediately after irradiation inhibited the 18 h u.v.B response in a dose-dependent manner. 7 The delayed onset microcirculatory vasodilatation induced by u.v.B involves both nitric oxide synthase and cyclo-oxygenase in this in vivo model. Topical corticosteroids may attenuate the response by inhibiting both prostaglandin and nitric oxide synthesis pathways.

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“…Many studies of responses of microvessels to vasoactive agents have appeared; a small sample includes studies of Hallback et al, 40 Altura, 141 Messina et al, 142 and Miller and Harris. 148 Unfortunately, the data have not been quantified satisfactorily in terms of local resistances in the networks examined.…”

mentioning

confidence: 99%

Report of the Hypertension Task Force of the National Heart, Lung, and Blood Institute. Current research and recommendations from the Subgroup on Local Hemodynamics.

1980

Hypertension

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Microcirculatory effects of prostaglandins E1, E2, and A1 in the rat mesentery and cremaster muscle

Cited by 51 publications

References 20 publications

Arachidonic acid and prostaglandin endoperoxide metabolism in isolated rabbit and coronary microvessels and isolated and cultivated coronary microvessel endothelial cells.

Arachidonic acid and prostaglandin endoperoxide metabolism in isolated rabbit and coronary microvessels and isolated and cultivated coronary microvessel endothelial cells.

Involvement of nitric oxide synthase in the delayed vasodilator response to ultraviolet light irradiation of rat skin in vivo

Report of the Hypertension Task Force of the National Heart, Lung, and Blood Institute. Current research and recommendations from the Subgroup on Local Hemodynamics.

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