Microcirculation alterations in experimentally induced gingivitis in dogs

Matsuo, Masato; Okudera, Toshimitsu; Takahashi, Shunsuke; Wada-Takahashi, Satoko; Maeda, Shingo; Iimura, Akira

doi:10.1007/s12565-015-0324-8

Cited by 12 publications

(11 citation statements)

References 20 publications

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“…Other studies on dogs have shown that predictable morphologic changes occur in the blood vessels at the gingival margin with the onset of inflammation. These vascular changes precede recognizable histopathological alterations, starting as early as 2 days after the induction of gingivitis [36,37,83].…”

Section: Microcirculation Of the Gingivamentioning

confidence: 96%

Laser Doppler Flowmetry Evaluation of the Microcirculation in Dentistry

Todea¹,

Canjau²,

Miron³

et al. 2016

Microcirculation Revisited - From Molecules to Clinical Practice

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This chapter presents the most important features of laser Doppler (LD) techniques: LD flowmetry (LDF) and LD imaging (LDI), together with examples of their clinical applications in dentistry. LDF gives a constant estimation of blood flow at a specified point, whereas LDI gives a 'snapshot' of perfusion at a given point. These methods are non-invasive laser-based techniques for monitoring gingival and pulpal blood flow and could be used as a diagnostic tool. In paediatric dentistry and odontology, LDF proved to be an atraumatic real-time method used for determining the tooth vitality by monitoring the pulp microcirculation in traumatized teeth, fractured teeth and teeth undergoing different conservative treatments (e.g. bleaching, dental preparation for prosthetic restorations, etc.). In periodontology, recent studies showed the ability of LDF to evaluate the health of gingival tissue in different types of periodontal diseases. By using LDF, it is also possible to evaluate the outcome after different periodontal treatments. The laser Doppler line scanning can be used for recording the gingival healing process after a surgical procedure in the anterior area of the oral cavity.

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Section: Microcirculation Of the Gingivamentioning

confidence: 96%

Laser Doppler Flowmetry Evaluation of the Microcirculation in Dentistry

Todea¹,

Canjau²,

Miron³

et al. 2016

Microcirculation Revisited - From Molecules to Clinical Practice

View full text Add to dashboard Cite

show abstract

“…Our previous study recently proved that functional analyses using a single index may not be accurate and should be accompanied by appropriate morphological analyses. 22 In this study, we demonstrated, by using morphological and physiological approach, that ultrasonic wave instruments cause minimal damage to gingival microcirculation, compared with a dental turbine.…”

Section: Discussionmentioning

confidence: 73%

“…[16][17][18][19][20][21] Recently, the LDF has been applied to determine blood flow alterations with high reproducibility in the maxillofacial region, including the gingival mucosa, and gingival vascular function. [22][23][24][25] The aim of this study was to determine whether ultrasonic preparation led to alterations in blood flow.…”

Section: Introductionmentioning

confidence: 99%

Microcirculation changes in gingival tissue after ultrasonic tooth preparation in beagle dogs

Matsuo

Wada-Takahashi

et al. 2020

J. Appl. Oral Sci.

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Microcirculation changes in gingival tissue after ultrasonic tooth preparation in beagle dogs Ultrasonic wave technology is widely used during dental treatments. We previously demonstrated that this method protects the gingival tissue. However, the physiological change on the gingival microvasculature caused by this method remains unclear. Objective: The aim of this study was to investigate the relationship between the morphological and physiological effects on gingival microcirculation when preparing teeth, using the conventional dental turbine or ultrasonic method. Methodology: The lower premolar teeth of beagle dogs were prepared along the gingival margin by using a dental turbine or ultrasonic wave instrument. Gingival vasculature changes were investigated using scanning electron microscopy for corrosion resin casts. Gingival blood flow at the preparation site was determined simultaneously by laser Doppler flowmetry. These assessments were performed immediately (Day 0), at 7 days and 30 days after tooth preparation. Results: At day 0, in the turbine group, blood vessels were destroyed and some resin leaked. Furthermore, gingival blood flow at the site was significantly increased. In contrast, the ultrasonic group demonstrated nearly normal vasculature and gingival blood flow similar to the non-prepared group for 30 days after preparation. No significant alterations occurred in gingival circulation 30 days after either preparation; however, the turbine group revealed obvious morphological changes. Conclusions: Based on multiple approach analyses, this study demonstrated that ultrasonic waves are useful for microvascular protection in tooth preparation. Compared with a dental turbine, ultrasonic wave instruments caused minimal damage to gingival microcirculation. Tooth preparation using ultrasonic wave instruments could be valuable for protecting periodontal tissue.

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“…The rats from each group were bled under pentobarbital sodium (45 mg/kg, intraperitoneally) anesthesia by perfusing 0.2% heparin in saline into the ascending aorta. The tissue was fixed and perfused in 10% neutral buffered formalin solution to maintain the vascular lumens [35]. After perfusing ink into the blood vessels, the maxilla was excised, hard tissue was decalcified using hydrochloric acid, and the specimens were embedded in paraffin to obtain 50-μm-thick sections.…”

Section: Morphological Analysismentioning

confidence: 99%

Effect of physical stimulation (gingival massage) on age-related changes in gingival microcirculation

et al. 2020

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The decline in circulatory function with aging may be alleviated by a combination of gingival massage (physical stimulation) and mechanical cleaning. Several studies have reported the systemic effect of physical stimulation on various parts of the body, including its therapeutic effect on pain in the neck and shoulders that becomes evident with age, and improvement in blood circulation. In contrast, few studies have reported on the changes in gingival microcirculation induced by gingival massage, while no previous study has evaluated the effect of gingival microcirculation on age-related changes in the hemodynamics of the oral cavity. This study aimed to investigate how gingival massage affects age-related changes in gingival microcirculation. Male Wistar rats (7-week, 6-month and 1-year old) were prepared for a gingival massage group and a control group. Mechanical stimulation was applied on the maxillary molar gingiva for 5 seconds twice a week for 4 weeks. Subsequently, gingival reactive hyperemia was measured using a laser Doppler flowmeter. In addition, morphological analyses were also performed by hematoxylin and eosin and Indian ink staining and a vascular resin cast model. Base Flow, maximum response (Peak), and time required for the maximum response to halve (T 1/2) were reduced in 1-year-old rats compared with the other age groups. In the mechanical stimulated group, T 1/2 was increased in 7-week, 6-month, and 1-year-old rats, and total blood flow (Mass) was increased in 6-month and 1-year-old rats. In addition, clear blood vessel networks and loop-like revascularization were only observed in the mechanical stimulated group. Changes in age-related decline in gingival microcirculatory function and vascular construction were reported in this study, and the results suggested that gingival massage activates both the functional and morphological aspects of gingival microcirculation and may be effective for maintaining oral health.

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Microcirculation alterations in experimentally induced gingivitis in dogs

Cited by 12 publications

References 20 publications

Laser Doppler Flowmetry Evaluation of the Microcirculation in Dentistry

Laser Doppler Flowmetry Evaluation of the Microcirculation in Dentistry

Microcirculation changes in gingival tissue after ultrasonic tooth preparation in beagle dogs

Effect of physical stimulation (gingival massage) on age-related changes in gingival microcirculation

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