Microchip for the Diagnosis of Cervical Cancer

Gulliksen, Anja; Karlsen, Frank

doi:10.1007/978-1-59745-426-1_6

Cited by 5 publications

(5 citation statements)

References 48 publications

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“…After statistical analysis, we were able to validate 28 upregulated and seven downregulated genes. Our findings represent a collection of cancer-related genes and a variety of genes associated with cell development and cell cycle mechanisms that have not been reported by other authors [8,[25][26][27][28].…”

Section: Discussionmentioning

confidence: 42%

Identification of differential expressed transcripts in cervical cancer of Mexican patients

et al. 2011

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The aim of this study was to identify the gene expression profile in biopsies of patients with cervical intraepithelial neoplasia (CIN) 1, CIN 2, CIN 3, and microinvasive cancer by suppression subtractive hybridization and Southern blotting. After analyzing 1,800 cDNA clones, we found 198 upregulated genes, 166 downregulated, and no significant change of gene expression in 86 clones (p = 0.005). These results were validated by Northern blot analysis (p = 0.0001) in the identification of 28 overexpressed and 7 downregulated transcripts. We observed a set of genes related to the Notch signaling pathway that may be involved in the transformation of cervical cells and in the development to malignancy. The differentially expressed genes may provide useful information about the molecular mechanisms involved in human cervical carcinoma and as diagnostic markers.

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Section: Discussionmentioning

confidence: 42%

Identification of differential expressed transcripts in cervical cancer of Mexican patients

et al. 2011

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“…In vitro testing of siRNA against cervical cancer has yielded promising results, but the same issues that hampered the clinical development of ribozymes and antisense oligonucleotides are now posing problems for the siRNA field: target selection, specificity, and delivery. If these problems are resolved, a variety of new and effective cervical cancer treatments could be available [184][185][186][187][188][189][190][191][192][193][194].…”

Section: Specimen Adequacymentioning

confidence: 99%

Genetic Screening of Cervical Cancer

Comparetto¹,

Borruto²

2021

obm genet

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Medical genetics plays an important role in the screening and prevention of numerous diseases. Thus, it is important to develop effective screening and prevention programs and improve the assessment of the susceptibility of diseases. The development of screening and prevention programs depends on the identification of early biomarkers (including functional and behavioral) for the risk and onset of the disease, and such programs need to be designed according to internationally accepted criteria. Cervical cancer represents a very relevant disease from the health and social perspective; around 528,000 new cases are diagnosed every year globally, of which, 85% are from developing countries, representing almost 12% of all cancers in females. Substantial reductions in the incidence of and mortality from cervical cancer have been observed after the introduction of prevention campaigns with the implementation of cervical screening programs through Papanicolaou (Pap) tests and, in particular, following the introduction of organized programs which guarantee a high level of screening coverage, as well as, the quality and continuity of diagnostic-therapeutic procedures. It is estimated that Pap smear screening every 3-5 years provides 80% protection against the onset of cancer. Advances in diagnostic techniques, particularly the development of easy-to-use molecular genetic tests, are replacing the use of the established Pap smear as a screening tool. This is possible owing to the discovery in 1975 that some cellular morphological changes (koilocytosis) were related to the presence of a Human Papillomavirus (HPV) infection. The HPV test is performed on a small sample of cells taken from the cervix, similar to the Pap test; however, it is not a morphological exam but a molecular biology exam that detects the presence of HPV by identifying its deoxyribonucleic acid (DNA) or messenger ribonucleic acid (mRNA). The results of numerous experimental studies have demonstrated a greater sensitivity of this test compared to the sensitivity of the traditional Pap test. However, the HPV test has a lower specificity due to two main factors: 1) The HPV test is based on the search for the types of viruses that have a greater oncogenic potential, and 2) It does not discriminate between transient infections and persistent and productive infections. The most widely used molecular tests are based on the search for HPV sequences and genotyping using molecular biology techniques, such as direct hybridization, qualitative polymerase chain reaction (PCR), and viral nucleotide sequencing.

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“…Além da replicação continua e descontrolada, também é típico que células tumorais sejam capazes de invadir e destruir tecidos vizinhos desrespeitando a organização e ordem biológica. Estas também podem migrar para outras partes do corpo através de vasos sanguíneos e linfáticos, de modo a se instalar em tecidos distantes, fenômeno conhecido como metástase (GULLIKSEN; KARLSEN, 2007).…”

Section: Introductionunclassified

Síntese de novos fosfolipídios antitumorais para a inibição da enzima trifosfato de citidina (CTP)

Antônio de Oliveira Neto

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Microchip for the Diagnosis of Cervical Cancer

Cited by 5 publications

References 48 publications

Identification of differential expressed transcripts in cervical cancer of Mexican patients

Identification of differential expressed transcripts in cervical cancer of Mexican patients

Genetic Screening of Cervical Cancer

Síntese de novos fosfolipídios antitumorais para a inibição da enzima trifosfato de citidina (CTP)

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