Microchip Emitter for Solid-Phase Extraction–Gradient Elution–Mass Spectrometry

Gasilova, Natalia; Qiao, Liang; Momotenko, Dmitry; Pourhaghighi, Mohammad Reza

doi:10.1021/ac400171e

Cited by 17 publications

(17 citation statements)

References 40 publications

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“…After preconcentration, analytes can be recovered for further processing or detected directly. Gasilova et al 15 used C18 functionalized magnetic beads integrated into a microfluidic device coupled to ESI-MS for peptide preconcentration. Microfluidic devices integrating a functionalized solid phase are promising devices for digestion, preconcentration or separation: Ethier et al 16 used SCX solid phase to make a proteomic reactor that allowed protein extraction, clean-up, preconcentration, and sample digestion.…”

Section: Introductionmentioning

confidence: 99%

On-Chip Sample Preparation Using a ChipFilter Coupled to NanoLC-MS/MS for Bottom-Up Proteomics

Ndiaye

Chiappetta

et al. 2020

J. Proteome Res.

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Sample preparation is a crucial step in bottom-up proteomics. Analytical performances of bottom-up proteomics can be improved by the miniaturization of sample preparation. Many microfluidic devices have been designed in the field of proteomics, but many of them are not capable of handling complex samples and do not integrate the processing and digestion steps. We propose a ChipFilter Proteolysis (CFP) microfluidic device as a proteomics reactor for the miniaturization of protein sample processing and digestion steps, whose design is closely related to the experimental setup of Filter Aided Sample Processing (FASP), even if no denaturing surfactant is required. The microchip has two reaction chambers of 0.6 µL volume separated by a protein filtration membrane in regenerated cellulose (10kD cut-off) that will concentrate or retain large polypeptides and will release small molecules. Cell lysis, protein concentration, and rapid chemical or enzymatic treatment can be performed in the ChipFilter. Complex proteomic samples like yeast protein extract or whole human cells proteome have been successfully analyzed with our microchip. Compared to the membranebased commercial ultracentrifugation cartridge, our microfluidic device offered a better proteome coverage with ten times less starting material and eight times faster protocol duration.

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Section: Introductionmentioning

confidence: 99%

On-Chip Sample Preparation Using a ChipFilter Coupled to NanoLC-MS/MS for Bottom-Up Proteomics

Ndiaye

Chiappetta

et al. 2020

J. Proteome Res.

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“…Strategy C produced the highest number of plasma protein groups and newly identified proteins among all the strategies tested. An SPE‐gradient elution‐MS microchip with a RP‐coated magnetic bead trap was designed for the detection and analysis of peptides of low concentration . The magnetic bead containing C8 and C18 chromatographic coatings trapped by permanent magnets was used as the SPE sorbent.…”

Section: Desaltingmentioning

confidence: 99%

Sample Clean‐up Strategies for ESI Mass Spectrometry Applications in Bottom‐up Proteomics: Trends from 2012 to 2016

2017

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Bottom-up proteomics is a mass spectrometric (MS)-based approach for the characterization of peptides obtained from in-solution protein digestion. MS is favored over other methods for peptide and protein analysis because of its better sensitivity and high throughput. Inorganic ions and surfactants present in the sample or produced during tryptic digestion are detrimental in MS analysis and affect the proteome data, thus sample preparation for removal of these unwanted components has become essential. Here, we review 48 research papers on strategies for removal of salts and surfactants (in particular, SDS) prior to ESI-MS analysis in bottom-up proteomics from 2012 to 2016. The strategies were mostly based on SPE and membrane-based filter-aided sample preparation for salt and SDS removal, respectively. Some known limitations of SPE and filter-aided sample preparation procedures are that they can be time consuming, laborious, and require the use of organic solvents before a concentrated extract suitable for analysis is obtained. The development of faster analytical methods by reducing the sample preparation time and thereby, increasing sample throughput, and in a solvent-less and membrane-less operation, is a significant contribution to proteome research.

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“…During the past few years, many integrated microfabricated devices have been developed to perform on‐chip sample pretreatment for MS analysis . Nordman et al integrated an on‐chip liquid‐phase microextraction (LPME) into a SU‐8 CE microchip for analyzing drug metabolites in urine samples.…”

Section: Recent Developments In Chip‐based Instrumentationmentioning

confidence: 99%

Microfluidic chip‐based liquid chromatography coupled to mass spectrometry for determination of small molecules in bioanalytical applications: An update

Lin

Fuh

2013

Electrophoresis

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Many microfluidic chip-based LC-MS systems have been utilized for high-throughput analysis in various fields of bioanalytical applications such as proteomic, glycomic, pharmaceutical, and clinical research. This review is an update of a previous review article (Electrophoresis 2012, 33, 635-643) to mainly cover the most recent advancements in chip-based LC-MS for determining small molecules in bioanalysis. First, the different types of microfluidic chip devices for chip-based LC-MS analysis will be discussed. Following the discussion of the recent developments in the chip-based instrumentation, the applications of chip-based LC-MS for determining small molecules, such as glycans, pharmaceutical drugs, drugs of abuse, drug metabolites, and biomarkers in various biological sample matrixes will also be included in this review.

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Microchip Emitter for Solid-Phase Extraction–Gradient Elution–Mass Spectrometry

Cited by 17 publications

References 40 publications

On-Chip Sample Preparation Using a ChipFilter Coupled to NanoLC-MS/MS for Bottom-Up Proteomics

On-Chip Sample Preparation Using a ChipFilter Coupled to NanoLC-MS/MS for Bottom-Up Proteomics

Sample Clean‐up Strategies for ESI Mass Spectrometry Applications in Bottom‐up Proteomics: Trends from 2012 to 2016

Microfluidic chip‐based liquid chromatography coupled to mass spectrometry for determination of small molecules in bioanalytical applications: An update

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