Microchip-based immunoassay system with branching multichannels for simultaneous determination of interferon-γ

Sato, Katsuaki; Yamanaka, Maho; Takahashi, Hiroyuki; Tokeshi, Manabu; Kimura, Hiroko; Kitamori, Takehiko

doi:10.1002/1522-2683(200203)23:5<734::aid-elps734>3.0.co;2-w

Cited by 192 publications

(116 citation statements)

References 25 publications

(37 reference statements)

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“…The detection signal intensity is proportional to the concentration of the antigen. Diffusion process has a great impact on the strength of the detection signal [24][25][26][27].…”

Section: Microfluidic-based Electrochemical Detection Technologymentioning

confidence: 99%

Microfluidic Electrochemical Detection Techniques of Cancer Biomarkers

Xie¹,

Chen²,

Lin³

2017

Nano BioMed ENG

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Microfluidic electrochemical sensors are based on the immune response. Immune charge density or electrode potential and other parameters sensitive interface causes a change or cause a power solution of the active ingredient (or their related components) to generate or consume, causing the detected current or potential. The electrochemical parameters of the law of changing enable the immune proteins quantitative or qualitative detection. Since the immune protein molecules themselves are typically non-electrochemical activity, and therefore immune electrochemical sensor usually mark the electrically active substance or enzyme in the antigen or antibody molecules in solution antigen (antibody) immune response and by the immobilized antigen (antibody) the concentration of the sample antigen (antibody) after the change of the current signal, causing the electro-active substance or enzyme-catalyzed reaction of substrate reaction can be determined indirectly.

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“…The detection signal intensity is proportional to the concentration of the antigen. Diffusion process has a great impact on the strength of the detection signal [24][25][26][27].…”

Section: Microfluidic-based Electrochemical Detection Technologymentioning

confidence: 99%

Microfluidic Electrochemical Detection Techniques of Cancer Biomarkers

Xie¹,

Chen²,

Lin³

2017

Nano BioMed ENG

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“…15,16,26,27 In our previous paper, 15 we reported on the possibility of an immunosorbent assay on a microchip. In this system, antigen-antibody reactions were performed on surfaces of microbeads packed in a microchannel with a dam structure (Fig.…”

Section: ·3 Immunoassaymentioning

confidence: 99%

“…To realize higher throughput analyses, a microchip system, which can process several samples simultaneously, was reported. 27 In this integrated system, the chip had branching multichannels and four reaction and detection regions; thus the system could process four samples at a time with only one pump unit (Fig. 10).…”

Section: ·3 Immunoassaymentioning

confidence: 99%

Integration of Chemical and Biochemical Analysis Systems into a Glass Microchip

et al. 2003

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This review focuses on the integration of chemical and biochemical analysis systems into glass microchips for general use. By combining multiphase laminar flow driven by pressure and micro unit operations, such as mixing, reaction, extraction and separation, continuous-flow chemical processing systems can be realized in the microchip format, while the application of electrophoresis-based chip technology is limited. The performances of several analysis systems were greatly improved by microchip integration because of some characteristics of microspace, i.e., a large specific interface area, a short molecular diffusion time, a small heat capacity and so on. By applying these concepts, several different analysis systems, i.e., wet analysis of cobalt ion, multi-ion sensor, immunoassay, and cellular analysis, were successfully integrated on a microchip. These microchip technologies are promising for meeting the future demands of highthroughput chemical processing.

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“…Recently, various microsystems targeted biomaterials, such as DNA [12][13][14] and protein [15][16][17][18][19] have been developed. These devices have been widely studied due to expectation as a robust tool in post-genome and/or clinical fields.…”

Section: Introductionmentioning

confidence: 99%

On-chip genotoxic bioassay based on bioluminescence reporter system using three-dimensional microfluidic network

Maehana

Tani

Kamidate

2006

Analytica Chimica Acta

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Microchip-based genotoxic bioassay using sensing Escherichia coli strains has been performed. In this method, the assay was conducted in three-dimensional microfluidic network constructed by a silicon perforated microwell array chip and two poly (dimethylsiloxane) (PDMS) multi-microchannel chips. The sensing strains having firefly luciferase reporter gene under transcriptional control of umuD as an SOS promoter were put into the channels on one of the PDMS chips and immobilized in the silicon microwells. Samples containing genotoxic substances and substrates for luciferase were into the channels on the other PDMS chip. The optimum conditions of the assay in the on-chip format have been investigated using mitomycin C (MMC) as a genotoxic substance. As a result, the dose-dependence of bioluminescence intensity was obtained at once on the chip. Additionally, the response ratios of the bioluminescence between mutagen-and non-induced strains were successfully enhanced by improving the on-chip assay methods and conditions. Several well-known genotoxic substances were subjected to the on-chip assay, and were detected with the detection limits comparable to those in the conventional method with reduced time.-3 -

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Microchip-based immunoassay system with branching multichannels for simultaneous determination of interferon-γ

Cited by 192 publications

References 25 publications

Microfluidic Electrochemical Detection Techniques of Cancer Biomarkers

Microfluidic Electrochemical Detection Techniques of Cancer Biomarkers

Integration of Chemical and Biochemical Analysis Systems into a Glass Microchip

On-chip genotoxic bioassay based on bioluminescence reporter system using three-dimensional microfluidic network

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