Microchemical investigation of medicinal plants. X

Polesuk, Jerry; Amodeo, Joseph M.; S., T.

doi:10.1007/bf01217995

Cited by 8 publications

(2 citation statements)

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“…); however, the exact composition is confidential, because it is one of the two Class-1 protected traditional Chinese Medicines [10,11]. Nevertheless, some studies tried to characterize the main active principles working in YB, revealing the presence of saponins, terpenoids and steroid glycosides [12,13]. Recently, Liu et al [11] reported the presence of five terpenoid saponins and eight steroidal saponins in YB powder and capsules that contribute to its therapeutic efficacy.…”

Section: Introductionmentioning

confidence: 99%

The Synergic Effect of Terpenoid and Steroidal Saponins Can Improve Bone Healing, by Promoting the Osteogenic Commitment of Adipose Mesenchymal Stem Cells: An In Vitro Study

et al. 2019

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Bone regeneration involves several biological processes that consistently impact the quality of tissue healing. An important step consists of the local recruitment and differentiation of mesenchymal stem cells that migrate in the site to regenerate from bone marrow. Mesenchymal stem cells (MSCs) may be pushed towards osteogenic commitment by specific substances, often naturally present in plants. Yunnan Baiyao (YB) is a Chinese herbal medicine, mainly working through the synergic effect of terpenoid and steroidal saponins. YB is well known for its numerous biomedical effects, including the ability to favor improved bone tissue healing. In our in vitro study, we used adipose mesenchymal stem cells (ADSCs) as a study-model: We selected samples to harvest and isolate ADSCs and investigate their viability; moreover, we performed bone-related gene expression to evaluate the differentiation of MSCs. To confirm this behavior, we analyzed alkaline phosphate activity and calcium deposition, with ADSCs cultured in basal and osteogenic media, with YB at different concentrations in the medium, and at different time-points: 7, 14 and 21 days. Our results indicate that the synergic effect of terpenoid and steroidal saponins slightly favor the late ADSCs differentiation towards the osteoblasts phenotype. In osteogenic committed cells, the treatment with the lower dose of YB promoted the up-regulation of the alkaline phosphatase gene (ALPL) at day seven and 14 (p < 0.01); at day 21, the alkaline phosphatase (ALP) activity showed a slight increase, although in basal condition it maintains low rates. We assume that such molecular synergy can promote the osteogenic commitment of adipose mesenchymal stem cells, thus improving the timing and the quality of bone healing.

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Section: Introductionmentioning

confidence: 99%

The Synergic Effect of Terpenoid and Steroidal Saponins Can Improve Bone Healing, by Promoting the Osteogenic Commitment of Adipose Mesenchymal Stem Cells: An In Vitro Study

et al. 2019

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“…As a result, in comparison with the development of clinical practice and pharmacological studies, component analysis and quality control has lagged far behind for YNBY. The pioneering work of Polesuk et al revealed the presence of saponins by microchemical method [10]. In later investigations by Li [11] and Liu et al [12], two triterpenoid cores were determined after sample hydrolysis and several steroid glycosides were detected.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of both triterpenoid and steroidal saponins in various Yunnan Baiyao preparations using HPLC–UV and HPLC–MS

Liu

Wang

Chen

et al. 2008

J of Separation Science

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Yunnan Baiyao (YNBY) is one of the best known traditional Chinese medicines. Saponins are considered to be its active components. In this study, an HPLC method was first developed for the simultaneous quantitative analysis of thirteen saponins, including five triterpenoid saponins and eight steroidal saponins, in a series of YNBY preparations, i. e., powder, capsules, aerosol, toothpaste, plaster, and adhesive bandage. The pre-treatment methods for each dosage form were investigated and optimized. The HPLC separation was performed on a Shim-pack C(18) reversed-phase column in gradient mode with UV detection at 203 nm. All calibration curves showed good linear regression (r(2) > or = 0.9981) within the test ranges. Precisions and repeatabilities of the methods were better than 4.22 and 4.78%, respectively. Recoveries were better than 90.5%, even in the analysis of the least abundant saponins in a complex YNBY plaster. HPLC-ESI-TOF/MS was used for definite identification of compounds in the preparations. This proposed method was successfully applied to quantify the 13 bioactive constituents in 27 commercial samples to evaluate the quality of YNBY preparations. The overall results demonstrate that this method is simple, reliable, and suitable for the quality control of YNBY. Furthermore, the retention behavior of these saponins in reversed-phase chromatography is described.

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