Microchannel wall coatings for protein separations by capillary and chip electrophoresis

Doherty, Erin A. S.; Meagher, Robert J.; Albarghouthi, Methal; Barron, Annelise E.

doi:10.1002/elps.200390029

Cited by 275 publications

(262 citation statements)

References 124 publications

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“…In particular, flow separation methods, such as chromatography, 1,2 electrophoresis, [3][4][5][6][7] and field flow fractionation (FFF), [8][9][10] have been extensively used in various fundamental and practical fields, and have facilitated the advancements of modern science. These methods cover separation of almost all classes of materials.…”

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confidence: 99%

Solute Distribution Coupled with Laminar Flow in Wide-Bore Capillaries: What Can Be Separated without Chemical or Physical Fields?

et al. 2005

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A number of researches have been devoted to the developments of novel separation principles and methodology, because material separation is one of the most important fundamentals in scientific and technological disciplines. In particular, flow separation methods, such as chromatography, 1,2 electrophoresis, [3][4][5][6][7] and field flow fractionation (FFF), [8][9][10] have been extensively used in various fundamental and practical fields, and have facilitated the advancements of modern science. These methods cover separation of almost all classes of materials. A number of dissolved molecules, from simple ions to macromolecules, can be separated by chromatography or electrophoresis, FFF and electrophoresis being capable of resolving even particles. The dimensions of solutes to be separated by these methods thus range from 10 -10 m to 10 -5 m. This situation may suggest that separation science and technology have been completely matured. However, studying new separation principles is still important, because they can allow the separation based on material properties that have not been utilized for conventional methods, provide higher performance and more efficient separation than existing means, and facilitate the understanding of materials through separation processes.According to chromatographic theories, the intrinsic separation performance of liquid chromatography is restricted in comparison with that of gas chromatography because of much lower solute diffusion in liquid phases. 11However, a liquid flow obviously provides versatile ways for material separation because liquids have higher density and dissolution power than gases, and thus has made possible application to various types of solutes with wide ranges in molecular weights and sizes. In the usual flow separation methods, appropriate chemical (chromatography) or physical (FFF) separation fields are created, which retain solutes to different extents and separate them. A variety of stationary phases for chromatography 2,[12][13][14] and physical forces for FFF [15][16][17][18][19] have been exploited, and have provided different capabilities and selectivity from those performed by conventional separation. In contrast, a separation method that requires neither chemical interactions nor special external fields should also be useful because of its instrumental simplicity. Hydrodynamic chromatography (HDC) is such a case, which allows separation of particles or macromolecules according to their hydrodynamic sizes just by passing them through a narrow channel. [20][21][22][23][24] Particles with larger diameter for example cannot approach the channel wall, and thus flow through the channel faster than the smaller particles, when the laminar flow profile is maintained therein. Although the applicability of this method was very restricted, recent developments of chip technology have brought about a remarkable progress. 23,24 If a capillary of radius a is used for HDC, the time for the elution of a particle of radius rp is given bywhere t0 is the time required ...

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confidence: 99%

Solute Distribution Coupled with Laminar Flow in Wide-Bore Capillaries: What Can Be Separated without Chemical or Physical Fields?

et al. 2005

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“…In general, the longer the sample is present in the electric-field the larger the separation that will occur. Some researchers try to eliminate EOF by modifying the channel walls prior to performing the separation [2]. Commonly, researchers report work where they modify buffer solutions to get the EOF to a fixed value that is favourable for their required separation.…”

Section: Controlling the Eof (And Zeta-potential)mentioning

confidence: 99%

Modelling New Techniques for Improving Separation in Miniature Capillary- and Planar-Based Capillary Electrophoresis Systems

Lewis

Harris

2017

Proceedings of Eurosensors 2017, Paris, France, 3–6 September 2017

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Abstract:The capillary/channel length is an important factor in capillary electrophoresis (CE) systems since it is directly related to the amount of separation attainable. In this work we present methods to increase the effective channel length without the need to modify the physical channel length. Using an electrode located close to the capillary surface it is possible to dynamically modify zeta-potential and therefore the electroosmotic flow (EOF). By controlling the EOF, certain ionic species within a sample can be held in a short channel whilst other species migrate along the channel. Alternatively the sample can be transported back and forth along the active channel length until sufficient separation has been attained. CE enables detailed analysis of a sample's composition and this is of interest to a range of applications.

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“…A number of dynamic coatings have been applied to fused-silica capillaries and microchips to reduce EOF and protein adsorption [39,40]. Besides aliphatic polyamines [41][42][43], alkylaminosilyl monomers [44] and oligoamines such as triethylenetetramine and diethylenetriamine [45,46] have also been used to reduce the surface charge and so EOF.…”

Section: Dynamic Wall Coatingmentioning

confidence: 99%

Capillary electrophoresis of proteins 2003–2005

Dolnı́k¹

2006

Electrophoresis

139

117

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This review article with 304 references describes recent developments in CE of proteins, and covers the two years since the previous review (Hutterer, K., Dolník, V., Electrophoresis 2003, 24, 3998-4012) through Spring 2005. It covers topics related to CE of proteins, including modeling of the electrophoretic migration of proteins, sample pretreatment, wall coatings, improving separation, various forms of detection, special electrophoretic techniques such as affinity CE, CIEF, and applications of CE to the analysis of proteins in real-world samples including human body fluids, food and agricultural samples, protein pharmaceuticals, and recombinant protein preparations.

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Microchannel wall coatings for protein separations by capillary and chip electrophoresis

Cited by 275 publications

References 124 publications

Solute Distribution Coupled with Laminar Flow in Wide-Bore Capillaries: What Can Be Separated without Chemical or Physical Fields?

Solute Distribution Coupled with Laminar Flow in Wide-Bore Capillaries: What Can Be Separated without Chemical or Physical Fields?

Modelling New Techniques for Improving Separation in Miniature Capillary- and Planar-Based Capillary Electrophoresis Systems

Capillary electrophoresis of proteins 2003–2005

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