Microcephaly protein Asp focuses the minus ends of spindle microtubules at the pole and within the spindle

Ito, Atsushi; Goshima, Gohta

doi:10.1083/jcb.201507001

Cited by 46 publications

(77 citation statements)

References 44 publications

(84 reference statements)

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“…When ATK5 was absent in root cells, spindles were less focused than they were in control cells [30]. However, whether the MT converging centres observed at the pole [23] are solely organised by the kinesin-14 motor remains unclear; in fly cells, this local crosslinking was dependent on an additional factor, ASPM/Asp [11,118], which is conserved, but uncharacterised, in plants.…”

Section: Molecular Mechanisms Of Spindle Assembly In Land Plantsmentioning

confidence: 99%

Mitotic Spindle Assembly in Land Plants: Molecules and Mechanisms

Yamada

Goshima

2017

Biology

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In textbooks, the mitotic spindles of plants are often described separately from those of animals. How do they differ at the molecular and mechanistic levels? In this chapter, we first outline the process of mitotic spindle assembly in animals and land plants. We next discuss the conservation of spindle assembly factors based on database searches. Searches of >100 animal spindle assembly factors showed that the genes involved in this process are well conserved in plants, with the exception of two major missing elements: centrosomal components and subunits/regulators of the cytoplasmic dynein complex. We then describe the spindle and phragmoplast assembly mechanisms based on the data obtained from robust gene loss-of-function analyses using RNA interference (RNAi) or mutant plants. Finally, we discuss future research prospects of plant spindles.

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Section: Molecular Mechanisms Of Spindle Assembly In Land Plantsmentioning

confidence: 99%

Mitotic Spindle Assembly in Land Plants: Molecules and Mechanisms

Yamada

Goshima

2017

Biology

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“…However, we lack reliable reporters to track single filament minus-ends in dividing cells. The recently described CAMSAP/patronin proteins only selectively label microtubule minus-ends in interphase cells, and other proteins (e.g., ASP) have only been shown to help locate the minus-ends of microtubule bundles [29,30,31]. Therefore, analyses of microtubule distributions have relied on indirect approaches, with many studies focusing on the metaphase spindle assembled in Xenopus egg extracts.…”

Section: The Dynamic Architecture Of the Metaphase Spindlementioning

confidence: 99%

Metaphase Spindle Assembly

Kapoor

2017

Biology

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“…Clearly, however, live imaging of the EB1-binding mutant of FIP (fip ∆1-2 ) revealed normal interzonal MT localization in anaphase and telophase ( Figure S4E, video 6); thus, interzonal MT recruitment is independent of EB1. We next investigated if FIP directly interacted with the MT binding protein Abnormal Spindle (Asp), which displays a similar anaphase localization to FIP (Asp; Riparbelli et al, 2002;Wakefield et al, 2001) and is known to bind MTs (Ito and Goshima, 2015;Mollinari et al, 2002;Saunders et al, 1997;Schoborg et al, 2015). However, we were unable to confirm a previously reported FIP-Asp interaction (Asp; Giot et al, 2003) using our Y2H system ( Figure S5).…”

Section: Fip Binds the Mt Lattice Via The Prc1 Ortholog Fascetto (Feo)mentioning

confidence: 78%

Fascetto Interacting Protein (FIP) Regulates Fascetto (PRC1) to Ensure Proper Cytokinesis and Ploidy

Swider

Varadarajan

et al. 2018

Preprint

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Cell division is critical for development, organ growth, and tissue repair. The later stages of cell division include the formation of the microtubule (MT)-rich central spindle in anaphase, which is required to properly define the cell equator, guide the assembly of the acto-myosin contractile ring, and ultimately ensure complete separation and isolation of the two daughter cells via abscission. Much is known about the molecular machinery that forms the central spindle, including proteins needed to generate the antiparallel overlapping interzonal MTs. One critical protein that has garnered great attention is Protein Regulator of Cytokinesis 1 (PRC1), or Fascetto (Feo) in Drosophila, which forms a homodimer to crosslink interzonal MTs, ensuring proper central spindle formation and cytokinesis. Here, we report on a new direct protein interactor and regulator of Feo we named Fascetto Interacting Protein (FIP). Loss of FIP results in a significant reduction in Feo localization, rapid disassembly of interzonal MTs, and several cytokinesis defects. Simultaneous reduction in Feo and FIP results in tumor-like, DNA-filled masses in the brain. In aggregate our data show that FIP functions upstream of, and acts directly on, Feo to ensure fully accurate cell division.

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Microcephaly protein Asp focuses the minus ends of spindle microtubules at the pole and within the spindle

Abstract: Asp localizes to the minus ends of spindle microtubules at the pole and within the spindle and cross-links them to adjacent microtubules.

Cited by 46 publications

References 44 publications

Mitotic Spindle Assembly in Land Plants: Molecules and Mechanisms

Mitotic Spindle Assembly in Land Plants: Molecules and Mechanisms

Metaphase Spindle Assembly

Fascetto Interacting Protein (FIP) Regulates Fascetto (PRC1) to Ensure Proper Cytokinesis and Ploidy

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