Microcarrier Culture

Abstract

Microcarrier culture provides an easily scalable approach to culture of anchorage‐dependent mammalian cells, providing a much higher surface area‐to‐volume ratio compared with roller bottles or flat layer cultivation systems. Microcarriers are also useful for the production of recombinant proteins, particularly to facilitate cell retention in cases where perfusion would be advantageous, for example, low specific productivity or unstable products. Microcarriers provide the cultivation surface that is necessary for adherent cells, and macroporous carriers can be used to immobilize suspension cells. In this review of microcarrier culture, we discuss the history of microcarrier use, strategies for optimal development of microcarrier‐based processes, the use of microcarriers for viral vaccine and recombinant protein production, and new biomedical applications for microcarriers, including tissue engineering, stem‐cell culture, and in vitro toxicology.

Combined macromolecule biomaterials together with fluid shear stress promote the osteogenic differentiation capacity of equine adipose-derived mesenchymal stem cells

“…It has been found that combined cultivation of chondrocytes with microcarriers in a 3D fashion facilitates phenotype maintenance by delivering mechanical stimulation [ 35 ]. Furthermore, MC has the ability to support cell viability and cell adhesion [ 36 ]. Although biomaterials play a role for cell survival and proliferation [ 37 , 38 ], it is questionable whether they also support MSCs differentiation.…”

3D Culture o Multiple Myeloma Cell Line Using Microgel Environments

“…One of the relevant parameters in the design of the 3D environment is the volume fraction occupied by the microspheres, which is expected to influence cell-material average distance and the dynamics of the system under stirring. This feature is also important for other 3D culture systems, as discussed above in Sections 1.5 and 1.6 (132).…”