Microcantilevers Modified by Horseradish Peroxidase Intercalated Nano-Assembly for Hydrogen Peroxide Detection

Yan, Xiaodong; Shi, Xiaolei; Hill, Kalisha; Ji, Hai‐Feng

doi:10.2116/analsci.22.205

Cited by 18 publications

(9 citation statements)

References 32 publications

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“…Interestingly, the results showed that the conformational change of the GOx enzyme upon complexation with glucose was the main origin of cantilever deflection [47]. Another recent publication further proved this concept [48].…”

Section: Enzyme-based MCL Biosensorsmentioning

confidence: 87%

Microcantilever (MCL) Biosensing

Yan¹,

Ji²,

Thundat

2006

CAC

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The analytical community continues to search for cost-effective, reliable, and even portable analytical techniques that can give reliable and fast-response results for a variety of biochemicals. Advances in the field of microelectro-mechanical systems (MEMS) and their uses now offer unique opportunities in the design of ultrasensitive analytical devices. Microcantilevers (MCLs) have been emerged as a novel unique platform for label-free biosensor or bioassay. This short review summarizes the biosensors using microcantilever (MCL) technology. DNA-based, antibody-based, enzyme-based, and membrane-based MCL sensors are discussed. The review is intended to provide an experts in this field an overview of MCL biosensors and to help the non-experts in the field to acquire a good understanding of the development of MCL biosensors and the main issues to be considered for further development of this biosensor technique. HISTORY AND MECHANISM OF MICRO-CANTILEVER (MCL) SENSORSSince the three pioneer papers published in 1994 [1-3], the MCL sensor technology had boomed and become a promising sensor technology. MCL sensors have several advantages over many other sensor technologies, including lower cost, lower point consumption, smaller size, the ability to detect multiple and identify various biochemical on a single chip. The SEM several pictures of MCLs are shown in Fig. (1).Cantilever responses, such as frequency, deflection, quality factor (Q-factor), and amplitude, undergo changes due to adsorption or changes in environment. Both frequency and bending approaches have been demonstrated to detect chemicals with sensitivity as high as parts-per-tillion to parts-per-quadrillion range and, each approach has its own advantages and disadvantages and each will be used for specific applications. For instance, resonance frequency of MCLs has been used to detect very low level pathogens [4,5]; bending approach showed special selectivity toward some chemicals because of it unique mechanism [6].Resonance frequency . The resonance frequency, f, of an oscillating cantilever can be expressed as (1) where K is the spring constant of the MCL and m* is the effective mass of the MCL. The effective mass can be related to the mass of the beam, m b , through the relation: m*=nm b , where n is a geometric parameter. It is clear that the resonance frequency can change due to changes in mass as well as changes in spring constant.Resonance frequency of a MCL (dynamic mode) can be used to detect chemical species in air. However, the oscillation of the cantilever is significantly dampened in aqueous solution, which reduces the quality factor and subsequent detection sensitivity of the MCLs in aqueous solutions. It should be noted that recent results showed that the second resonance frequency of MCLs remains sharp in aqueous solutions [4] and can be used for detecting biomolecules in solutions.

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Section: Enzyme-based MCL Biosensorsmentioning

confidence: 87%

Microcantilever (MCL) Biosensing

Yan¹,

Ji²,

Thundat

2006

CAC

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“…Similar phenomena have been observed on other enzymes, such as organophosphorus hydrolase (OPH) 41 and horseradish peroxidase (HRP). 42 In both cases, calculations showed that the conformational changes of the enzymes contribute greatly to cantilever bending.…”

Section: Surface Stress Change Resulted From Conformational Change Of...mentioning

confidence: 99%

Microcantilever biosensors based on conformational change of proteins

Gao

Buchapudi

et al. 2008

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Microcantilevers (MCLs) hold a position as a cost-effective and highly sensitive sensor platform for medical diagnostics, environmental analysis and fast throughput analysis. MCLs are unique in that adsorption of analytes on the microcantilever (MCL) surface changes the surface characteristics of the MCL and results in bending of the MCL. Surface stress due to conformation change of proteins and other polymers has been a recent focus of MCL research. Since conformational changes in proteins can be produced through binding of anylates at specific receptor sites, MCLs that respond to conformational change induced surface stress are promising as transducers of chemical information and are ideal for developing microcantilever-based biosensors. The MCL can also potentially be used to investigate conformational change of proteins induced by non-binding events such as post-translational modification and changes in temperature or pH. This review will provide an overview of MCL biosensors based on conformational change of proteins bound to the MCL surface. The models include conformational change of proteins, proteins on membranes, enzymes, DNA and other polymers.

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“…Detection of hydrogen peroxide is both of industry interests and of biological importance. Yan et al developed 44,39 MCL sensors for H 2 O 2 detection. The MCLs were modified by horseradish peroxidase (HRP) through a layer-by-layer nanoassembly technique.…”

Section: Hydrogen Peroxide (H 2 O 2 )mentioning

confidence: 99%

Microcantilever biosensors for chemicals and bioorganisms

Buchapudi

Huang

Yang

et al. 2011

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In the last fifteen years, microcantilevers (MCLs) have been emerging as a sensitive tool for the detection of chemicals and bioorganisms. Because of their small size, lightweight, and high surface-to-volume ratio, MCL-based sensors improve our capability to detect and identify biological agents by orders of magnitude. A biosensor is a device for the detection of an analyte that combines a biological component with a physicochemical detector component. The MCL biosensors have recently been reviewed in several papers. All of these papers were organized based on the sensing biological elements (antibody, enzyme, proteins, etc.) for recognition of analytes. In this review, we intend to summarize the microcantilever biosensors in a format of each specific chemical and bioorganism species to make information on individual biosensors easily accessible. We did this to aid researchers to locate relevant references.

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Microcantilevers Modified by Horseradish Peroxidase Intercalated Nano-Assembly for Hydrogen Peroxide Detection

Cited by 18 publications

References 32 publications

Microcantilever (MCL) Biosensing

Microcantilever (MCL) Biosensing

Microcantilever biosensors based on conformational change of proteins

Microcantilever biosensors for chemicals and bioorganisms

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