Microbispora cellulosiformans sp. nov., a novel actinomycete with cellulase activity isolated from soil in the cold region

Gong, Xiujie; Xiang, Wensheng; Cao, Xu; Yang, Yu; Hao, Yunhong; Li, Liang; Wang, Qiuju; Zou, Hongtao; Qian, Chunrong

doi:10.1007/s10482-020-01477-4

Cited by 13 publications

(6 citation statements)

References 37 publications

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“… a , Data from Kaewkla et al [5]; b , Data from Gong et al [7]. …”

Section: Resultsmentioning

confidence: 99%

“…f [5]. The species ‘ Microbispora tritici ’ [6] and ‘ Microbispora cellulosiformans ’ [7] which were isolated from the root of wheat ( Triticum aestivum L.) and the soil of the cold region, respectively, have been described recently but their names are not yet validly published. Our laboratory has been interested in isolating actinobacteria from soils, plants, sediments, insects and so on, with the purpose of selecting strains with potential for biotechnological applications.…”

Section: Introductionmentioning

confidence: 99%

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Microbispora sitophila sp. nov., a novel actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.)

Guo

et al. 2019

International Journal of Systematic and Evolutionary Microbiology

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A novel actinobacterium, designated strain NEAU-D428T, was isolated from rhizosphere soil of wheat and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics of the strain coincided with members of the genus Microbispora . The 16S rRNA gene sequence analysis showed that the isolate was most closely related to Microbispora bryophytorum NEAU-TX2-2T (99.2 %). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain clustered with Microbispora clausenae CLES2T (99.1 %), but formed a separate subclade in the phylogenomic tree within the genus Microbispora . The menaquinones were identified as MK-9(H4), MK-9(H2) and MK-9(H0). The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, ninhydrin-positive glycophospholipid, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were identified as iso-C16 : 0, C16 : 0, 10-methyl C17 : 0 and C18 : 0. Digital DNA–DNA hybridization and average nucleotide identity values between strain NEAU-D428T and M. bryophytorum NEAU-TX2-2T, Microbispora camponoti 2C-HV3T, M. clausenae CLES2T, ‘Microbispora cellulosiformans’ Gxj-6T and Microbispora fusca NEAU-HEGS1-5T were 47.6 and 92.2 %, 47.5 and 92.2 %, 55.9 and 94.0 %, 33.1 and 86.8 %, and 33.6 and 87.1 %, respectively. These results and some physiological and biochemical properties demonstrated that the strain could be distinguished from its closest relatives. Therefore, it is proposed that strain NEAU-D428T should be classified as representative of a novel species of the genus Microbispora , for which the name Microbispora sitophila sp. nov. is proposed. The type strain is NEAU-D428T (=CGMCC 4.7523T=DSM 109822T).

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“… a , Data from Kaewkla et al [5]; b , Data from Gong et al [7]. …”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microbispora sitophila sp. nov., a novel actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.)

Guo

et al. 2019

International Journal of Systematic and Evolutionary Microbiology

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“…An inoculum of 5 mL of SKI-[H], SKI-[Z] and SKI-[X] strain suspension was added into 1000 mL of ISP-2 broth (containing 5 g of yeast extract, 10 g of malt extract, 6 g of dextrose and a pH of 7.3). The combination was thereafter left to incubate for 25 days at 150 rpm [15]. The fermented broth was centrifuged for 10 min at 4 ºC and 10,000 revolutions per minute to extract secondary metabolites.…”

Section: Initial Assessment Of Antibacterial Activity Of the Identifi...mentioning

confidence: 99%

Synthesis and Chemical Characterization of Secondary Metabolites of Weeds using FTIR and NMR Techniques

Izhar,

Rizvi,

Shahab

et al. 2024

ajc

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Soil microorganisms have the ability to produce bioactive chemicals that can be utilized in the development of a bioherbicide for the purpose of biological weed control. A total of 34 bacterial strains were isolated and tested for their herbicidal activity against both grass and broadleaf weeds. From this group, six effective isolates were chosen for the purpose of characterization and identification. The actinomycete isolates were identified as Microbacterium sp and Streptomyces sp. based on an analysis of their morphological, biochemical and physiological characteristics. Out of the initially chosen 6 strains, the bacterial fermentation broths from one particular isolate strain exhibited herbicidal activity that resulted in a 95% reduction in the growth of weed when compared to the control. The Streptomycetes isolates underwent a germination inhibition assay to determine the presence of herbicidal properties. A total of 8 crop seeds were examined for herbicidal efficacy using Streptomycetes isolates and no growth inhibition was observed in the crop seeds. The herbicidal activity of Streptomyces isolates was tested on four weed seeds. Streptomyces inhibits the growth of nut grass. The current investigation establishes that Streptomyces isolates has the potential to function as a bioherbicide against nut grass. The strain SKI-[Z] was shown to have a 99% resemblance to Actinobacterial spp. through phylogenetic study of 16S rRNA gene sequencing. The FT-IR spectrum shows alkynes (-C≡C), alkenes (=C-H), amines (N-H), aliphatic amine (C-N) and alkanes (-C-H) and the NMR peak having ketone groups (C=O), di-ketone group (>C=O), ether (R-O-R) and aldehyde group (-CHO). Furthermore, upon 1H NMR analysis of the secondary metabolites, the prominent peaks intensities at δ 2.4, 3.68 and 4.1 ppm correspond to the ketone groups (C=O), di-ketone group (>C=O), ether (R-O-R) and aldehyde group (-CHO) were found.

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“…Bacterial enzymes possess key features more advantageous than fungal enzymes such as higher growth rates, genetic versatility ( Menendez, Garcia-Fraile & Rivas, 2015 ) and more specific, efficient catalytic activity in harsh conditions; moreover, bacteria produce multienzyme complexes with greater functionality and specificity in broader conditions ( Balla et al, 2022 ; Castiglia et al, 2016 ; Maki, Leung & Qin, 2009 ). Cellulases from actinomycetes associated to insects and other extreme and marine prokaryotes have exhibited high specific activity, thermostability, and other important biochemical properties and hence can contend well with the enzymes from terrestrial sources ( Behera et al, 2017 ; Elframawy et al, 2022 ; Fathallh Eida et al, 2012 ; Gong et al, 2017 ; Gong et al, 2020 ; Goodfellow et al, 2018 ; Lewin et al, 2016 ; Saini et al, 2015 ; Walia et al, 2017 ; Xie & Pathom-Aree, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Systematic bioprospection for cellulolytic actinomycetes in the Chihuahuan Desert: isolation and enzymatic profiling

Escudero-Agudelo,

Martínez-Villalobos,

Arocha-Garza

et al. 2023

PeerJ

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The quest for microbial cellulases has intensified as a response to global challenges in biofuel production. The efficient deconstruction of lignocellulosic biomass holds promise for generating valuable products in various industries such as food, textile, and detergents. This article presents a systematic bioprospection aimed at isolating actinomycetes with exceptional cellulose deconstruction capabilities. Our methodology explored the biodiverse oligotrophic region of Cuatro Cienegas, Coahuila, within the Chihuahuan Desert. Among the evaluated actinomycetes collection, 78% exhibited cellulolytic activity. Through a meticulous screening process based on enzymatic index evaluation, we identified a highly cellulolytic Streptomyces strain for further investigation. Submerged fermentation of this strain revealed an endoglucanase enzymatic activity of 149 U/mg. Genomic analysis of strain Streptomyces sp. STCH565-A revealed unique configurations of carbohydrate-active enzyme (CAZyme) genes, underscoring its potential for lignocellulosic bioconversion applications. These findings not only highlight the significance of the Chihuahuan Desert as a rich source of cellulolytic microorganisms but also offer insights into the systematic exploration and selection of high-performing cellulolytic microorganisms for application in diverse environmental contexts. In conclusion, our bioprospecting study lays a foundation for harnessing the cellulolytic potential of actinomycetes from the Chihuahuan Desert, with implications for advancing cellulose deconstruction processes in various industries. The findings can serve as a blueprint for future bioprospecting efforts in different regions, facilitating the targeted discovery of microorganisms with exceptional cellulosic deconstruction capabilities.

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Microbispora cellulosiformans sp. nov., a novel actinomycete with cellulase activity isolated from soil in the cold region

Cited by 13 publications

References 37 publications

Microbispora sitophila sp. nov., a novel actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.)

Microbispora sitophila sp. nov., a novel actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.)

Synthesis and Chemical Characterization of Secondary Metabolites of Weeds using FTIR and NMR Techniques

Systematic bioprospection for cellulolytic actinomycetes in the Chihuahuan Desert: isolation and enzymatic profiling

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