Microbiotyping the Sinonasal Microbiome

Bassiouni, Ahmed; Paramasivan, S.; Shiffer, Arron; Dillon, Matthew R.; Cope, Emily K.; Cooksley, Clare; Ramezanpour, Mahnaz; Moraitis, Sophia; Ali, Mohammad Javed; Bleier, Benjamin S.; Callejas, Claudio; Cornet, Marjolein; Douglas, Richard; Dutra, Daniel Lorena; Georgalas, Christos; Harvey, Richard J.; Hwang, Peter H.; Luong, Amber U.; Schlosser, Rodney J.; Tantilipikorn, Pongsakorn; Tewfik, Marc A.; Vreugde, Sarah; Wormald, Peter John; Caporaso, J. Gregory; Psaltis, Alkis James

doi:10.3389/fcimb.2020.00137

Cited by 24 publications

(17 citation statements)

References 66 publications

(86 reference statements)

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“…However, the abundance of Moraxella was significant decreased in the NECRSwNP group compared with that in the ECRSwNP group. Although M. catarrhalis may contribute to chronic rhinosinusitis (Hoggard et al, 2017a;Bassiouni et al, 2020), Moraxella is not the main factor for NECRSwNP (Figure 4). Infants dominated by Hemophilus had higher rates of respiratory illness and a predisposition to asthma, likely due to the inflammatory potential of Hemophilus (Teo et al, 2015;Bosch et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Sinus Microbiota in Patients With Eosinophilic and Non-Eosinophilic Chronic Rhinosinusitis With Nasal Polyps

Feng

Miao

Liu

et al. 2021

Front. Cell. Infect. Microbiol.

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Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by Th2-skewed inflammation and increased colonization by Staphylococcus aureus. CRSwNP can be distinguished as eosinophilic (ECRSwNP) and non-eosinophilic (NECRSwNP) by the infiltration of eosinophils. The local microbiota plays an important role in the persistent inflammation of CRSwNP. To evaluate the bacterial community composition on the distinct types of CRSwNP patients, we collected nasal swabs from 16 ECRSwNP patients, 18 NECRSwNP patients, and 39 healthy control subjects. The microbiome structure for all the samples were analyzed by high-throughput 16S rRNA gene sequencing. Concentration of S. aureus was determined using TaqMan quantitative polymerase chain reaction (qPCR) targeting the nuclease (nuc) gene. The result showed significant differences in the sinus microbiome among healthy control subjects and CRSwNP patients. Microbiota community diversity was significantly lower in NECRSwNP samples compared to that of healthy control subjects. Interestingly, the abundance of several pathogenic bacteria was diverse between ECRSwNP and NECRSwNP patients. Although Staphylococcus prevailed in all groups, the abundance of Staphylococcus was significantly higher in the healthy control group than the ECRSwNP group. More importantly, the abundance of S. aureus was much higher in NECRSwNP patients. This study highlights that microbiota composition may contribute to the different clinical types of CRSwNP, inspiring new therapeutic strategies to resolve this chronic inflammation process.

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Section: Discussionmentioning

confidence: 99%

Sinus Microbiota in Patients With Eosinophilic and Non-Eosinophilic Chronic Rhinosinusitis With Nasal Polyps

Feng

Miao

Liu

et al. 2021

Front. Cell. Infect. Microbiol.

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“…Previous studies on the microbiome in CRS have mostly focused on the comparison of the microbial composition of patients with CRS (CRS with and without polyps) with that of healthy subjects [ 27 , 28 , 29 , 30 , 31 , 32 ]. In addition, some studies have investigated patients with CRS with clinical variables, including asthma, cystic fibrosis, geographical locations, and the relationship between the heterogeneity of CRS and microbiome dysbiosis [ 27 , 33 , 34 ]. However, the results of these studies are controversial.…”

Section: Introductionmentioning

confidence: 99%

Microbiome of Unilateral Chronic Rhinosinusitis: A Controlled Paired Analysis

Park

Lee

et al. 2021

IJERPH

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The sinonasal microbiota in human upper airway may play an important role in chronic rhinosinusitis (CRS). Thus, this study aimed to investigate the human upper airway microbiome in patients with unilateral CRS, and compare the sinonasal microbiome of the unilateral diseased site with that of a contralateral healthy site. Thirty samples, 15 each from the diseased and healthy sites, were collected from the middle meatus and/or anterior ethmoid region of 15 patients with unilateral CRS during endoscopic sinus surgery. DNA extraction and bacterial microbiome analysis via 16S rRNA gene sequencing were then performed. Corynebacterium showed the highest relative abundance, followed by Staphylococcus in samples from both the diseased and healthy sites. Further, the relative abundances of Staphylococcus and Pseudomonas were significantly lower in samples from diseased sites than in those from healthy sites. Conversely, anaerobes, including Fusobacterium, Bacteroides, and Propionibacterium, were abundantly present in samples from both sites, more so in samples from diseased sites. However, the sites showed no significant difference with respect to richness or diversity (p > 0.05). Our results indicate that CRS might be a polymicrobial infection, and also suggest that Corynebacterium and Staphylococcus may exist as commensals on the sinus mucosal surface in the upper respiratory tract.

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“…This was well demonstrated that a potential reciprocal relationship between Corynebacterium sps, S . aureus , and other players reaches an equilibrium to maintain a healthy state [ 68 ]. These correlations were observed in our study as well.…”

Section: Discussionmentioning

confidence: 99%

Composition of nasal bacterial community and its seasonal variation in health care workers stationed in a clinical research laboratory

2021

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The microorganisms at the workplace contribute towards a large portion of the biodiversity a person encounters in his or her life. Health care professionals are often at risk due to their frontline nature of work. Competition and cooperation between nasal bacterial communities of individuals working in a health care setting have been shown to mediate pathogenic microbes. Therefore, we investigated the nasal bacterial community of 47 healthy individuals working in a clinical research laboratory in Kuwait. The taxonomic profiling and core microbiome analysis identified three pre-dominant genera as Corynebacterium (15.0%), Staphylococcus (10.3%) and, Moraxella (10.0%). All the bacterial genera exhibited seasonal variations in summer, winter, autumn and spring. SparCC correlation network analysis revealed positive and negative correlations among the classified genera. A rich set of 16 genera (q < 0.05) were significantly differentially abundant (LEfSe) across the four seasons. The highest species counts, richness and evenness (P < 0.005) were recorded in autumn. Community structure profiling indicated that the entire bacterial population followed a seasonal distribution (R2-0.371; P < 0.001). Other demographic factors such as age, gender and, ethnicity contributed minimally towards community clustering in a closed indoor laboratory setting. Intra-personal diversity also witnessed rich species variety (maximum 6.8 folds). Seasonal changes in the indoor working place in conjunction with the outdoor atmosphere seems to be important for the variations in the nasal bacterial communities of professionals working in a health care setting.

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Microbiotyping the Sinonasal Microbiome

Cited by 24 publications

References 66 publications

Sinus Microbiota in Patients With Eosinophilic and Non-Eosinophilic Chronic Rhinosinusitis With Nasal Polyps

Sinus Microbiota in Patients With Eosinophilic and Non-Eosinophilic Chronic Rhinosinusitis With Nasal Polyps

Microbiome of Unilateral Chronic Rhinosinusitis: A Controlled Paired Analysis

Composition of nasal bacterial community and its seasonal variation in health care workers stationed in a clinical research laboratory

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