Microbiote shift in the Medicago sativa rhizosphere in response to cyanotoxins extract exposure

Khalloufi, Fatima El; Oufdou, Khalid; Bertrand, Mathieu; Lahrouni, Majida; Oudra, Brahim; Ortet, Philippe; Barakat, Mohamed; Heulin, Thierry; Achouak, Wafa

doi:10.1016/j.scitotenv.2015.08.127

Cited by 25 publications

(22 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, the species is proved to behave as an endophytic strain colonizing all plant compartments, besides being a root symbiont of legumes [15]. Also, PCR-based 454 pyrosequencing confirmed the decreasing bacterial diversity from unplanted soil to root tissues of Lucerne, and that the root tissues were mainly inhabited by Alphaproteobacteria associated by Gammaproteobacteria, as well as Betaproteobacteria, Flavobacteria and Actinobacteria, which may have some additional plant-growth-promoting activities [14,16]. Of interest was the study of Schwieger and Tebbe [17] who compared the products of cultivated isolates and community profiles as determined by Cultivation-independent-SSCP (Single Strand Conformation Polymorphism) and indicated that the most dominant groups identified by cultivation were also detected in the community profiles.…”

Section: Introductionmentioning

confidence: 99%

Plant-fed versus chemicals-fed rhizobacteria of Lucerne: Plant-only teabags culture media not only increase culturability of rhizobacteria but also recover a previously uncultured Lysobacter sp., Novosphingobium sp. and Pedobacter sp.

et al. 2017

View full text Add to dashboard Cite

In an effort to axenically culture the previously uncultivable populations of the rhizobacteria of Lucerne (Medicago sativa L.), we propose plant-only teabags culture media to mimic the nutritional matrix available in the rhizosphere. Here, we show that culture media prepared from Lucerne powder teabags substantially increased the cultivability of Lucerne rhizobacteria compared with a standard nutrient agar, where we found that the cultivable populations significantly increased by up to 60% of the total bacterial numbers as estimated by Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Cluster analysis of 16S rDNA Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) of cultivable Colony-Forming Units (CFUs) revealed a more distinct composition and separation of bacterial populations recovered on the plant-only teabags culture media than those developed on a standard nutrient agar. Further, the new plant medium gave preference to the micro-symbiont Sinorhizobium meliloti, and succeeded in isolating a number of not-yet-cultured bacteria, most closely matched to Novosphingobium sp., Lysobacter sp. and Pedobacter sp. The present study may encourage other researchers to consider moving from the well-established standard culture media to the challenging new plant-only culture media. Such a move may reveal previously hidden members of rhizobacteria, and help to further explore their potential environmental impacts.

show abstract

Section: Introductionmentioning

confidence: 99%

Plant-fed versus chemicals-fed rhizobacteria of Lucerne: Plant-only teabags culture media not only increase culturability of rhizobacteria but also recover a previously uncultured Lysobacter sp., Novosphingobium sp. and Pedobacter sp.

et al. 2017

View full text Add to dashboard Cite

show abstract

“…They could be modifi ed by lower or higher density of the algae cells. The fact that foliar application of the studied strains increased corn growth could be caused by greater intensity of several physiological events and by plant enrichment with phytohormones, auxin (IAA), GA, cytokinins, amino acids, macronutrients (N, P, K, Ca, Mg), microelements (S, Zn, Fe, Mn, Cu, Mo, Co), polyamines, and several other secondary metabolites that can be produced by Cyanobacteria and by nitrogen assimilated by these microorganisms from the atmosphere, as described in plants of several species grown in favorable conditions by Haroun and Hussein [18], Nain et al [43], Masojídek and Prášil [19], Chojnacka et al [20], Nunnery et al [21], Perez-Garcia et al [22], Pszczolkowski et al [23], Rana et al [44], Sahu et al [33], Markou and Nerantzis [24], Grzesik and Romanowska-Duda [8], Michalak et al [45], Corbel et al [46 47], Mazur-Marzec [48], and El Khalloufi et al [49].…”

Section: Discussionmentioning

confidence: 99%

How Biojodis and Cyanobacteria Alleviate the Negative Influence of Predicted Environmental Constraints on Growth and Physiological Activity of Corn Plants

Piotrowski¹,

Romanowska-Duda²,

Grzesik³

2016

Pol. J. Environ. Stud.

View full text Add to dashboard Cite

Information concerning corn plant development within predicted environmental constraints and the possibility of alleviating their negative infl uence by ecological compounds is limited. Thus the aim of our research was to evaluate the physiological activity, growth, and development of corn plants under the expected climate-change conditions and treated with Biojodis (certifi cated extract of humus and active iodine; Jodavita, Lithuania) and Cyanobacteria (a mixture of monocultures of Microcystis aeruginosa MKR 0105 and Anabaena sp. PCC 7120). 'Cyrkon' corn plants (Zea mays L.) were cultivated in several simulated predicted climate conditions, including constant or periodically changing temperature and soil moisture content. During the vegetative season they were treated at three-week intervals using Biojodis at concentrations of 1.0, 2.0, or 3.0%, and Cyanobacteria, and then assessed using growth and physiological parameters.The obtained results showed the corn's different reactions to the applied conditions. Plants that grew at constant or periodically changing different temperatures (0 to 40ºC) or in unsatisfactorily (20% m.c.) or excessively (60% m.c.) watered soils, developed slowly in comparison with those growing at 20ºC and in optimally moistened media (30% m.c.). Watering, spraying, or watering and spraying of corn plants with Biojodis (1, 2, 3%) and a foliar application of Cyanobacteria increased their growth at optimal or unfavourable temperature and in unsatisfactory or excessively moistened soil. The three times spraying with Biojodis (1 or 2%) and with Cyanobacteria was most promising. The plant development changes related to the used compounds and simulated environmental constraints were associated with the changes in indexes of chlorophyll content in leaves, stability of membranes, gas exchange, and activity of acid or alkaline phosphatase and RNase. The above indicates that foliar and soil application of Biojodis and plant sprayings with Cyanobacteria increased growth of corn cultivated under optimal temperature and soil

show abstract

“…The results revealed fluctuations with an increase in Betaproteobacteria and a decrease in Gammaproteobacteria proportion. Furthermore, cyanobacterial extract containing microcystins used for irrigation seemed to be toxic towards Actinobacteria, Gemmatimonas, Deltaproteobacteria and Gammaproteobacteria, however other groups as Clostridia, Opitutae and bacteria related with Betaproteobacteria, were stimulated [109]. However, Lahrouni et al [110,111] reported that rhizobia-Vicia faba symbiosis was not impacted by microcystins.…”

Section: Microorganismsmentioning

confidence: 99%

“…In contrast, these studies revealed a stimulation of the potential of nitrification that was positively correlated to an increase in the abundance of ammonia-oxidizing bacteria, whereas the ammonia-oxidizing archaea were not impacted. In a recently study, El Khalloufi et al [109] highlighted the effects of cyanobacterial extract containing microcystins on soil microorganisms from the rhizosphere of Medicago sativa. The authors exposed M. sativa to 100 µg equivalent MC-LR L −1 during 30 days at three times a week and a pyrosequencing analysis was further established to characterize the bacterial community of the rhizosphre.…”

Section: Microorganismsmentioning

confidence: 99%

Cyanobacterial Toxins Emerging Contaminants in Soils: A Review of Sources, Fate and Impacts on Ecosystems, Plants and Animal and Human Health

Bouaı̈cha¹,

Corbel²

2016

Soil Contamination - Current Consequences and Further Solutions

View full text Add to dashboard Cite

In the last few decades, there has been a growing interest in the occurrence of cyanotoxins and their potential toxicity in the aquatic environment. However, the used of dried toxic cyanobacteria cells as fertilizer or the used of surface water contaminated with cyanotoxins for agricultural crops irrigation can be source of soil contamination. In addition, surface waters presenting dense toxic blooms of cyanobacteria and used for agricultural practices are not controlled and are often used without prior treatment. Once in soil, cyanotoxins may be transported again to water bodies by leaching, runoff and drainage processes or can be accumulated in soils and, therefore, may cause contamination of vegetation by absorption from soils or by surface pollution of plants. In addition to possible effects on human health, elevated levels of cyanotoxins in soils can negatively affect plant vigour, animal health, microbial processes and overall soil health. Consequently, the focus of this chapter of soil contamination is cyanotoxins as contaminants of emerging concern in the soil, identifying sources of contamination, determining their fate and effects in the soil, and understanding their bioaccumulation in agricultural plants used for feed and food and consequences on animal and human health.

show abstract

Microbiote shift in the Medicago sativa rhizosphere in response to cyanotoxins extract exposure

Cited by 25 publications

References 69 publications

Plant-fed versus chemicals-fed rhizobacteria of Lucerne: Plant-only teabags culture media not only increase culturability of rhizobacteria but also recover a previously uncultured Lysobacter sp., Novosphingobium sp. and Pedobacter sp.

Plant-fed versus chemicals-fed rhizobacteria of Lucerne: Plant-only teabags culture media not only increase culturability of rhizobacteria but also recover a previously uncultured Lysobacter sp., Novosphingobium sp. and Pedobacter sp.

How Biojodis and Cyanobacteria Alleviate the Negative Influence of Predicted Environmental Constraints on Growth and Physiological Activity of Corn Plants

Cyanobacterial Toxins Emerging Contaminants in Soils: A Review of Sources, Fate and Impacts on Ecosystems, Plants and Animal and Human Health

Contact Info

Product

Resources

About