Microbiota of <i>Telenomus tridentatus</i> (Platygastroidea: Scelionidae): An unwanted parasitoid

Ramírez‐Ahuja, María de Lourdes; Gómez-Govea, Mayra A.; Lugo‐Trampe, Ángel; Borrego‐Soto, Gissela; Delgado‐Enciso, Iván; Ponce‐García, Gustavo; Martínez‐Fierro, Margarita L.; Ramírez-Valles, Eda Guadalupe; Treviño, Víctor; Flores‐Suarez, Adriana E.; Rodríguez‐Sánchez, Iram P.

doi:10.1111/jen.12656

Cited by 4 publications

(2 citation statements)

References 65 publications

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“…Additionally, relative abundance of Staphylococcus was above 0.5% only in one replicate out of three (Table S5) and it did not appear in the MR microbiome. This bacterium has been repeatedly reported as a gut microbe in insects (e.g., wasp, 47 parasitoid wasp, 43 aphids and moths, 48 Beetles, 49 termites 50 ). No substantial association between arthropods and Carnimonas has been previously reported (although it was found in surveys of surroundings of honeybees 51,52 ), and since our attempts to detect it by diagnostic PCRs have failed, we concluded that it was a single case of contamination or a ‘by‐ passer’.…”

Section: Discussionmentioning

confidence: 87%

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Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Izraeli

Lalzar

Netanel

et al. 2020

Pest Management Science

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BACKGROUND Like numerous other animals, biocontrol agents (BCAs) of arthropod pests carry various microorganisms that may have diverse effects on the biology of their eukaryote hosts. We postulated that it is possible to improve the efficacy of BCAs by manipulating the composition of their associated microbiota. The parasitoid wasp Anagyrus vladimiri (Hymenoptera: Encyrtidae) from a mass‐rearing facility was chosen for testing this hypothesis. RESULTS High‐throughput sequencing analysis indicated that fungal abundance in A. vladimiri was low and variable, whereas the bacterial community was dominated by the endosymbiont Wolbachia. Wolbachia was fixed in the mass‐rearing population, whereas in field‐collected A. vladimiri Wolbachia's prevalence was only approximately 20%. Identification of Wolbachia strains from the two populations by Multi Locus Sequence Typing, revealed two closely related but unique strains. A series of bioassays with the mass‐rearing Wolbachia‐fixed (W+) and a derived antibiotic‐treated Wolbachia‐free (W–) lines revealed that: (i) Wolbachia does not induce reproductive manipulations; (ii) W– females have higher fecundity when reared individually, but not when reared with conspecifics; (iii) W+ females outcompete W– when they share hosts for oviposition; (iv) longevity and developmental time were similar in both lines. CONCLUSIONS The findings suggest that W+ A. vladimiri have no clear fitness benefit under mass‐rearing conditions and may be disadvantageous under lab‐controlled conditions. In a broader view, the results suggest that augmentative biological control can benefit from manipulation of the microbiome of natural enemies.

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Section: Discussionmentioning

confidence: 87%

“…Only a handful of papers reported the characterization of parasitoids' microbiome by molecular methods 43–46 . In those, a diverse community of bacteria was found, most of which was attributed to environmental microbes, associated with gut activity or transient.…”

Section: Discussionmentioning

confidence: 99%

Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Izraeli

Lalzar

Netanel

et al. 2020

Pest Management Science

View full text Add to dashboard Cite

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Bacterial Communities Associated with Megalopyge opercularis (Smith) (Lepidoptera: Megalopygidae): Exploring Poisonous Lepidopterans

Gómez-Govea

Ramírez‐Ahuja

Castellanos-López

et al. 2023

Florida Entomologist

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Thrips Microbiome Study in Commercial Avocado (Persea americana Mill.) from Northwest Colombian Andes (Antioquia, Colombia) Shows the Presence of Wolbachia, Ehrlichia, Enterobacter

et al. 2022

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Microbiota associated with insects play several important roles in their host, including protection against pathogens, provision of nutrition, and survival in hostile environments. The aim of this work was to identify the bacterial community found in avocado thrips from Northwestern Colombia (Antioquia department) in order to find isolates for potential biocontrol purposes. Culture-dependent methods based on 16S rRNA and gyrase B gene sequencing in 42 bacterial isolates allowed the identification of the genera Bacillus, Serratia, Moraxella, Pantoea, and Sphingomonas. Microbial diversity detected with the temperature gradient gel electrophoresis (TGGE) technique on three morphotypes of thrips, named brown (Scirtothrips hansoni), black (Frankliniella panamensis), and pale (Frankliniella sp.), showed a low bacterial community density (Shannon–Wiener index = 1480, p > 0.05) with significant differences among morphotypes (R = 0.7877, p = 0.0004). Results obtained with Illumina sequencing on the V1–V2 hypervariable region of the subunit 16S rRNA showed a predominant sequence in the brown morphotype (Scirtothrips hansoni) that belongs to the genus Wolbachia. The 16S amplicon analyses were extended to more samples and higher resolution using the V4–V5 hypervariable region. The results showed six additional bacteria phyla, confirming the previous observation for the dominant bacterial groups made in S. hansoni and the detection of the alternation of highly predominant genera among these thrips. Our results demonstrate that endosymbiont such as Wolbachia sp. are part of the microbiota of these pests, thereby indicating the possibility of employing this type of bacterium to improve the management of avocado thrips globally.

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Microbiota of Telenomus tridentatus (Platygastroidea: Scelionidae): An unwanted parasitoid

Cited by 4 publications

References 65 publications

Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Bacterial Communities Associated with Megalopyge opercularis (Smith) (Lepidoptera: Megalopygidae): Exploring Poisonous Lepidopterans

Thrips Microbiome Study in Commercial Avocado (Persea americana Mill.) from Northwest Colombian Andes (Antioquia, Colombia) Shows the Presence of Wolbachia, Ehrlichia, Enterobacter

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