Microbiota Landscape of Gut System of Guppy Fish (<i>Poecilia reticulata</i>) Plays an Outstanding Role in Adaptation Mechanisms

Kayath, Christian Aimé; Zamba, Armel Ibala; Goma-Tchimbakala, Joseph; Mamonékéné, Victor; Makanga, Gloire Moniceth Mombo; Lebonguy, Aimé Augustin; Nguimbi, Etienne

doi:10.1155/2019/3590584

Cited by 17 publications

(18 citation statements)

References 44 publications

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“…The emulsification index is a direct method for demonstrating the ability of strains to produce biosurfactants or not [13]. Those molecules have been known to form emulsions between two immiscible liquids [34, 35].…”

Section: Discussionmentioning

confidence: 99%

“…An overnight of 5mL of bacterial culture have been done. The emulsification index (EI24) was calculated as an indicator for BLM as previously demonstrated [13]. The medium was adjusted to pH 7.2 and supplemented with gasoline or diesel fuel (1 mL for 300 mL of medium).…”

Section: Methodsmentioning

confidence: 99%

“…A previous study in our laboratory that showed that Shigella sp. isolated from Brazzaville wastewater were able to emulsify hydrocarbon from gasoline and/or diesel fuel [13]. Sachin et al found the same profile of hydrocrabon emulsification with Shigella strain [14].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Invasion of epithelial cells are correlated with secretion of Biosurfactant via the type 3 secretion system (SST3) ofShigella flexneri

Kinavouidi

Kayath²,

Nguimbi

2020

Preprint

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11Biosurfactants are amphipathic molecules produced by many microorganisms, usually bacteria, 12 fungi and yeasts. They possess the property of reducing the tension of the membrane interfaces. 13 No studies have been conducted on Shigella species showing their involvement of biosurfactant 14 like molecules (BLM) in pathogenicity. This study aims to show that environmental and clinical 15 strains of Shigella are able to produce BLM by emulsifying gasoline and diesel fuels. Our study 16 has shown that BLM are secreted in the extracellular medium with EI24 ranging from 80 to 17 100%. The secretion is depending on the type III secretion system (T3SS). We did show that S. 18 flexneri, S. boydii and S. sonnei are able to interact with hydrophobic areas with respectively 19 17.64%, 21.42% and 22.22% of hydrophobicity. 100 mM Benzoic and 1.5mg/mL Salycilic 20 acids have been inhibited T3SS and this totally stops the BLM secretion. Pseudomonas 21 aeruginosa which has T3SS is able to produce 100% of BLM in the presence or in the absence 22 of both T3SS inhibitors. The secreted BLM is extractable with an organic solvent such as 23 chloroform and could entirely be considered like lipopeptide or polypeptidic compound. By 24 secreting BLM, Shigella is able to perform multicellular phenomena like "swarming" allowing 25 to invade and disseminate inside epithelial cells. 26 Introduction 28 The ingestion of pathogenic and virulent microorganisms generally affecting peoples in both 29 developed and developing countries [1]. Shigella is one of the Gram-negative bacterium 30 belonging to Enterobacteriaceae family and is causative agent of bacillary dysentery or 31 shigellosis [2]. The genus Shigella was the major pathogen bacteria associated with dysentery 32with attributable fraction to 63,8%, but also the second most common pathogen associated with 33 watery diarrhoea with attributable fraction to 12,9% in sub-Saharan Africa and south Asia. 34 Children under 5 years are the most affected. More and more shigellosis is a pathology that 35 both towards neglected diseases but 164300 of death per years have been notified all over the 36 world in 2010. Most deaths occur in sub-Saharan Africa and in south Asia [3-6]. This is include 37 Republic of Congo and surprisingly no epidemiological studies have been conducted in this 38 field. The genus Shigella includes four species (S. flexneri, S. sonnei, S. dysenteriae and S. 39 boydii) [7]. 10 bacteria of S. dysenteriae type 1 and 100 to 180 bacteria of S. flexneri or S. 40 sonnei are enough to produce symptomatic infection [8].41Shigella's pathogenicity is based on a virulence plasmid pWR100 in which the mxi-spa locus 42 encodes the type three secretion system(T3SS) involved in effector production like IpaB, C and 43 D (Translocator and Tip) to invade host cell [9][10][11][12]. A previous study in our laboratory that 44 showed that Shigella sp. isolated from Brazzaville wastewater were able to emulsify 45 hydrocarbon from gasoline and/or diesel fuel [13]. Sachin et al. found the same pr...

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Invasion of epithelial cells are correlated with secretion of Biosurfactant via the type 3 secretion system (SST3) ofShigella flexneri

Kinavouidi

Kayath²,

Nguimbi

2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…From 5 mL of bacterial overnight culture, the emulsification index (EI24) was calculated as an indicator for BLM production as previously demonstrated [ 5 ]. The medium was adjusted to pH 7.2 and supplemented with gasoline or diesel fuel (1 mL for 300 mL of medium).…”

Section: Methodsmentioning

confidence: 99%

“…A previous study in our laboratory showed that Shigella sp. isolated from Brazzaville wastewater were able to emulsify hydrocarbon from gasoline and/or diesel fuel [5]. According to amphipathic features, biosurfactants display a variety of surface activities, which explain their application in several fields related with emulsification, foaming, detergency, wetting, dispersion, pathogenicity, and solubilisation of hydrophobic compounds [6,7].…”

Section: Introductionmentioning

confidence: 99%

Invasion of Epithelial Cells Is Correlated with Secretion of Biosurfactant via the Type 3 Secretion System (T3SS) of Shigella flexneri

Kinavouidi

Kayath

Nguimbi

2020

Journal of Pathogens

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Biosurfactants are amphipathic molecules produced by many microorganisms, usually bacteria, fungi, and yeasts. They possess the property of reducing the tension of the membrane interfaces. No studies have been conducted on Shigella species showing the role of biosurfactant-like molecules (BLM) in pathogenicity. The aim of this study is to assess the ability of Shigella environmental and clinical strains to produce BLM and investigate the involvement of biosurfactants in pathogenicity. Our study has shown that BLM are secreted in the extracellular medium with EI24 ranging from 80% to 100%. The secretion is depending on the type III secretion system (T3SS). Moreover, our results have shown that S. flexneri, S. boydii, and S. sonnei are able to interact with hydrophobic areas with 17.64%, 21.42%, and 22.22% hydrophobicity, respectively. BLM secretion is totally prevented due to inhibition of T3SS by 100 mM benzoic and 1.5 mg/ml salicylic acids. P. aeruginosa harboring T3SS is able to produce 100% of BLM in the presence or in the absence of both T3SS inhibitors. The secreted BLM are extractable with an organic solvent such as chloroform, and this could entirely be considered a lipopeptide or polypeptide compound. Secretion of BLM allows some Shigella strains to induce multicellular phenomena like “swarming.”

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Metagenomic landscape of taxonomy, metabolic potential and resistome of Sardinella longiceps gut microbiome

2021

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Microbiota Landscape of Gut System of Guppy Fish (Poecilia reticulata) Plays an Outstanding Role in Adaptation Mechanisms

Cited by 17 publications

References 44 publications

Invasion of epithelial cells are correlated with secretion of Biosurfactant via the type 3 secretion system (SST3) ofShigella flexneri

Invasion of epithelial cells are correlated with secretion of Biosurfactant via the type 3 secretion system (SST3) ofShigella flexneri

Invasion of Epithelial Cells Is Correlated with Secretion of Biosurfactant via the Type 3 Secretion System (T3SS) of Shigella flexneri

Metagenomic landscape of taxonomy, metabolic potential and resistome of Sardinella longiceps gut microbiome

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