Microbiota intestinal de larvas e adultos de Anastrepha fraterculus (Wiedemann, 1830) (Diptera: Tephritidae): diversidade e efeito do alimento

Abstract

companhia, ajuda e conhecimento compartilhado; Aos colegas e conhecidos que tornaram o dia a dia mais alegre e minha nova querência mais acolhedora; Aos ótimos amigos que conquistei desde 2011, que compartilharam amizade, companheirismo, ensinamentos e respeito; E aos familiares e amigos de longa data que, mesmo de longe sempre, me apoiaram e incentivaram.

Gut Bacteriome Analysis of Anastrepha fraterculus sp. 1 During the Early Steps of Laboratory Colonization

“…From these classified taxonomic units, 29 OTUs were described at the genus level, one OTU (OTU19) remains unclassified under phylum level, identified as unknown Saccharibacteria and two other OTUs (15 and Overall analyses suggested that the gut bacterial profile was dominated by Proteobacteria, particularly by Alpha and Gammaproteobacteria; followed by Firmicutes and Saccharibacteria. Within the Gammaproteobacteria, the Enterobacteriaceae family was dominant in the gut of A. fraterculus, in agreement with previous reports in Tephritidae fruit flies (Behar et al, 2008;Jurkevitch, 2011;Müller, 2013;Morrow et al, 2015;Ventura et al, 2018;Augustinos et al, 2019;Koskinioti et al, 2019). The most abundant genera, were Wolbachia (Alphaproteobacteria) and Enterobacter (Gammaproteobacteria), followed by other members of the Gammaproteobacteria such as Providencia, Aeromonas, Citrobacter, Burkholderia-Caballeronia-Paraburkholderia, Klebsiella and Raoultella.…”

“…For A. fraterculus, Proteobacteria dominate the gut bacterial community according to Augustinos et al (2019) and our results. However, Müller (2013) found that the gut bacteriome of a Brazilian wild population of A. fraterculus was dominated by Actinobacteria. This author targeted a different region of the 16S rRNA gene, which could partially explain the differences; however, the main explanation is probably associated to environmental variation (Augustinos et al, 2019).…”

“…These species are considered pests because larvae develop inside a wide range of fruit species including many commercial ones (White and Elson-Harris, 1992). In most tephritid fruit flies examined so far, Enterobacteriaceae has been recognized as the dominant taxonomic group, also including a range of culturable and unculturable bacteria associated to the different life styles of the host species (Behar et al, 2008;Jurkevitch, 2011;Müller, 2013;Augustinos et al, 2015;Ventura et al, 2018). A greater richness of digestive symbiotic species is associated to polyphagous flies including microbes belonging to Proteobacteria and Firmicutes phyla (Morrow et al, 2015).…”

Analysis of the Gut Bacterial Community of Wild Larvae of Anastrepha fraterculus sp. 1: Effect of Host Fruit, Environment, and Prominent Stable Associations of the Genera Wolbachia, Tatumella, and Enterobacter

“… Müller (2013) found that the larval gut bacteriome of a Brazilian wild population of A. fraterculus was dominated by Actinobacteria. In this case, the author targeted a different region of the 16S rRNA gene, which could partially explain the differences; however, the fact that the morphotype of A. fraterculus studied is not indicated by Müller (2013) together with environmental variation, such as diet, might contribute to the differences observed with our study as well as with previous results ( Augustinos et al, 2019 ; Salgueiro et al, 2020 ). For other Anastrepha species, a study carried out based on 454 pyrosequencing analysis, reported that the gut of larvae was dominated by Escherichia in A. striata, A. ludens and A. obliqua , and by Raoultella in A. serpentina ( Ventura et al, 2018 ).…”

“…1 adults (Augustinos et al, 2019;Juárez et al, 2019;Salgueiro et al, 2020). Müller (2013) found that the larval gut bacteriome of a Brazilian wild population of A. fraterculus was dominated by Actinobacteria. In this case, the author targeted a different region of the 16S rRNA gene, which could partially explain the differences; however, the fact that the morphotype of A. fraterculus studied is not indicated by Müller (2013) together with environmental variation, such as diet, might contribute to the differences observed with our study as well as with previous results (Augustinos et al, 2019;Salgueiro et al, 2020).…”