Microbiota dispersion in the Uyuni salt flat (Bolivia) as determined by community structure analyses

Perez-Fernandez, Cesar; Iriarte, Mercedes; Rivera-Pérez, Jessica I.; Tremblay, Raymond L.; Toranzos, Gary A.

doi:10.1007/s10123-018-00052-2

Cited by 6 publications

(8 citation statements)

References 48 publications

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“…Salar de Uyuni is characterized by high salinity, with salt concentration values in the range of 132-177 g L −1 [57], which coupled with relatively low pH values (4.6-5.8) results in an extremely harsh habitat for living organisms. For surviving in that environment, endemic microorganisms develop self-protection strategies including the production of extracellular biopolymers [19,24,58]. Additionally, it has been theorized that the presence of metallic species in the form of salts contribute largely to both the microbial diversity found in Salar de Uyuni and to its biotechnological potential [56].…”

Section: Discussionmentioning

confidence: 99%

Exopolysaccharides Production by Cultivating a Bacterial Isolate from the Hypersaline Environment of Salar de Uyuni (Bolivia) in Pretreatment Liquids of Steam-Exploded Quinoa Stalks and Enzymatic Hydrolysates of Curupaú Sawdust

et al. 2021

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The halotolerant bacterial strain BU-4, isolated from a hypersaline environment, was identified as an exopolysaccharide (EPS) producer. Pretreatment liquids of steam-exploded quinoa stalks and enzymatic hydrolysates of Curupaú sawdust were evaluated as carbon sources for EPS production with the BU-4 strain, and the produced EPS was characterized using FTIR, TGA, and SEM. Cultivation was performed at 30 °C for 48 h, and the cells were separated from the culture broth by centrifugation. EPS was isolated from the cell pellets by ethanol precipitation, and purified by trichloroacetic acid treatment, followed by centrifugation, dialysis, and freeze-drying. EPS production from quinoa stalks- and Curupaú sawdust-based substrates was 2.73 and 0.89 g L−1, respectively, while 2.34 g L−1 was produced when cultivation was performed on glucose. FTIR analysis of the EPS revealed signals typical for polysaccharides, as well as ester carbonyl groups and sulfate groups. High thermal stability, water retention capacity and gel-forming ability were inferred from SEM and TGA. The capability of the halotolerant isolate for producing EPS from pretreatment liquids and hydrolysates was demonstrated, and characterization of the EPS revealed their broad application potential. The study shows a way for producing value-added products from waste materials using a bacterium from a unique Bolivian ecosystem.

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Section: Discussionmentioning

confidence: 99%

Exopolysaccharides Production by Cultivating a Bacterial Isolate from the Hypersaline Environment of Salar de Uyuni (Bolivia) in Pretreatment Liquids of Steam-Exploded Quinoa Stalks and Enzymatic Hydrolysates of Curupaú Sawdust

et al. 2021

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“…The microbial community reported in this study was rich in species but was dominated by only a few of them, as previously suggested in SdU (Rubin et al ., 2017; Pérez‐Fernández et al ., 2019) and in other salt flats from the lithium triangle (Aguilar et al ., 2016, 2018; Farias et al ., 2017; Núñez Salazar et al ., 2020). Interestingly, the best adapted species to this polyextreme environment, members of Salinibacter , Halonotius and Halorubrum genera, are characterized by their pigmentation (Oren, 2013; DasSarma et al ., 2019; Durán‐Viseras et al ., 2019), an effective protection against the intense UV radiation affecting SdU (Gomariz et al ., 2015).…”

Section: Discussionmentioning

confidence: 96%

“…The SdU microbial diversity has been described recently by means of methodologies based on 16S rRNA. Using a limited number of samples, these studies have shown that the microbial community of SdU is dominated by members of the archaeal phylum Euryarchaeota, specifically of the Halonotius genus (Rubin et al ., 2017; Haferburg et al ., 2017; Pérez‐Fernández et al ., 2019; Pecher et al ., 2020) and members of the phylum Bacteroidetes, particularly Salinibacter genus (Rubin et al ., 2017; Pérez‐Fernández et al ., 2019; Ramos‐Barbero et al ., 2019; Pecher et al ., 2020). The viral analysis of SdU pointed out the presence of phages, some of them specific for members of these two genera (Ramos‐Barbero et al ., 2019).…”

Section: Introductionmentioning

confidence: 99%

Subsurface and surface halophile communities of the chaotropic Salar de Uyuni

Martínez

Escudero

Rodríguez

et al. 2021

Environmental Microbiology

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Salar de Uyuni (SdU) is the biggest athalosaline environment on Earth, holding a high percentage of the known world Li reserves. Due to its hypersalinity, temperature and humidity fluctuations, high exposure to UV radiation, and its elevated concentration of chaotropic agents like MgCl 2 , LiCl and NaBr, SdU is considered a polyextreme environment. Here, we report the prokaryotic abundance and diversity of 46 samples obtained in different seasons and geographical areas. The identified bacterial community was found to be more heterogeneous than the archaeal community, with both communities varying geographically. A seasonal difference has been detected for archaea. Salinibacter, Halonotius and Halorubrum were the most abundant genera in Salar de Uyuni. Different unclassified archaea were also detected. In addition, the diversity of two subsurface samples obtained at 20 and 80 m depth was evaluated and compared with the surface data, generating an evolutionary record of a multilayer hypersaline ecosystem.

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“…Cyanobium_PCC-6307, Aeromonas and Pseudomonas are the dominant genera in C1-3, but are barely detectable in C4-5 (Figure 4C). Among them, genus Salinibacter is an extreme halophilic genus with archaeal properties in Bacteroidetes, also an important part of bacterial communities in various high-salt environments (Bachran et al, 2019;Perez-Fernandez et al, 2019;Cycil et al, 2020). Salinibacter spp.…”

Section: Discussionmentioning

confidence: 99%

“…At present, high-throughput sequencing technology has been applied to investigate microbial communities in different hypersaline environments (Genderjahn et al, 2018;Perez-Fernandez et al, 2019;Zhu et al, 2020). Compared to traditional methods, it has emerged as a reliable tool for investigating differences in microbial community species diversity and structure in any given habitat (Boutaiba et al, 2011), because the culture-independent method of amplicon sequencing appears to be more efficient than the traditional culture-dependent methods (Gibtan et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Microbial community structure and shift pattern of industry brine after a long-term static storage in closed tank

Luo

et al. 2022

Front. Microbiol.

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Brine from Dingyuan Salt Mine (Anhui, China), an athalassohaline hypersaline environment formed in the early tertiary Oligocene, is used to produce table salt for hundreds of millions of people. However, halophiles preserved in this niche during deposition are still unknown. Here, we employed cultivation and high-throughput sequencing strategies to uncover the microbial community and its shift after a long-term storage in the brine collected from Dingyuan Salt Mine. High-throughput sequencing showed (1) in the fresh brine (2021), Cyanobium_stocktickerPCC-6307 spp. (8.46%), Aeromonas spp. (6.91%) and Pseudomonas spp. (4.71%) are the dominant species in bacteria while Natronomonas spp. (18.89%), Halapricum spp. (13.73%), and Halomicrobium spp. (12.35%) in archaea; (2) after a 3-year-storage, Salinibacter spp. (30.01%) and Alcanivorax spp. (14.96%) surpassed Cyanobium_stocktickerPCC-6307 spp. (8.46%) becoming the dominant species in bacteria; Natronomonas spp. are still the dominant species, while Halorientalis spp. (14.80%) outnumbered Halapricum spp. becoming the dominant species in archaea; (3) Alcanivorax spp. and Halorientalis spp. two hydrocarbons degrading microorganisms were enriched in the brine containing hydrocarbons. Cultivation using hypersaline nutrient medium (20% NaCl) combined with high-throughput 16S rRNA gene sequencing showed that (1) the biomass significantly increased while the species diversity sharply declined after a 3-year-storage; (2) Halorubrum spp. scarcely detected from the environment total stocktickerDNA were flourishing after cultivation using AS-168 or NOM medium; (3) twelve possible new species were revealed based on almost full-length 16S rRNA gene sequence similarity search. This study generally uncovered the microbial community and the dominant halophiles in this inland athalassohaline salt mine, and provided a new insight on the shift pattern of dominant halophiles during a long-term storage, which illustrated the shaping of microorganisms in the unique environment, and the adaptation of microbe to the specific environment.

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Microbiota dispersion in the Uyuni salt flat (Bolivia) as determined by community structure analyses

Cited by 6 publications

References 48 publications

Exopolysaccharides Production by Cultivating a Bacterial Isolate from the Hypersaline Environment of Salar de Uyuni (Bolivia) in Pretreatment Liquids of Steam-Exploded Quinoa Stalks and Enzymatic Hydrolysates of Curupaú Sawdust

Exopolysaccharides Production by Cultivating a Bacterial Isolate from the Hypersaline Environment of Salar de Uyuni (Bolivia) in Pretreatment Liquids of Steam-Exploded Quinoa Stalks and Enzymatic Hydrolysates of Curupaú Sawdust

Subsurface and surface halophile communities of the chaotropic Salar de Uyuni

Microbial community structure and shift pattern of industry brine after a long-term static storage in closed tank

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