Microbiota-derived acetate enables the metabolic fitness of the brain innate immune system during health and disease

Erny, Daniel; Dokalis, Nikolaos; Mezö, Charlotte; Castoldi, Ângela; Mossad, Omar; Staszewski, Ori; Frosch, Maximilian; Villa, Matteo; Fuchs, Vidmante; Mayer, Arun; Neuber, Jana; Sosat, Janika; Tholen, Stefan; Schilling, Oliver; Vlachos, Andreas; Blank, Thomas; Agüero, Mercedes Gomez de; Macpherson, Andrew J.; Pearce, Edward J.; Prinz, Marco

doi:10.1016/j.cmet.2021.10.010

Cited by 183 publications

(117 citation statements)

References 68 publications

Supporting

Mentioning

110

Contrasting

Order By: Relevance

“… Continue with a volume of 350 μL from the previous step and add 3.5 μL Antimycin A working solution (working solution concentration: 2 mM → final concentration: 20 μM), vortex and record the Δψm for 90 s ( Figure 3 D). Optional: Comparison of microglial cells derived from healthy control and diseased conditions, such as neurodegenerative mouse models (e.g., 5×FAD mice) can be performed ( Erny et al., 2021 ) (MX-O4 + microglia from 5×FAD; Figure S7D). …”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…Optional: Comparison of microglial cells derived from healthy control and diseased conditions, such as neurodegenerative mouse models (e.g., 5×FAD mice) can be performed ( Erny et al., 2021 ) (MX-O4 + microglia from 5×FAD; Figure S7D).…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…This protocol is useful for evaluating mitochondrial CI- and CII-mediated respiratory activity. As shown in Figures 2 and 3 and in ( Erny et al., 2021 ), the Δψm can be robustly modulated by the compounds enabling comprehensive analysis of the respiratory chain function. In Figures 3 and 4 we assessed CII function which can be inhibited by increasing concentrations of the endogenous CII inhibitor OAA ( Figure 4 ).…”

Section: Expected Outcomesmentioning

confidence: 97%

“…This optimized protocol requires a low input of permeabilized cells and can be applied to other ex vivo isolated cells or cells derived from cell cultures. For complete details on the use and execution of this protocol, please refer to Erny et al. (2021) .…”

mentioning

confidence: 99%

“…For complete details on the use and execution of this protocol, please refer to Erny et al. (2021) .…”

mentioning

confidence: 99%

See 4 more Smart Citations

Flow-cytometry-based protocol to analyze respiratory chain function in mouse microglia

et al. 2022

Self Cite

View full text Add to dashboard Cite

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Section: Expected Outcomesmentioning

confidence: 97%

mentioning

confidence: 99%

“…For complete details on the use and execution of this protocol, please refer to Erny et al. (2021) .…”

mentioning

confidence: 99%

See 3 more Smart Citations

Flow-cytometry-based protocol to analyze respiratory chain function in mouse microglia

et al. 2022

Self Cite

View full text Add to dashboard Cite

Nutritional metabolism and cerebral bioenergetics in Alzheimer's disease and related dementias

Yassine

Self

Kerman

et al. 2022

Alzheimer's & Dementia

View full text Add to dashboard Cite

Disturbances in the brain's capacity to meet its energy demand increase the risk of synaptic loss, neurodegeneration, and cognitive decline. Nutritional and metabolic interventions that target metabolic pathways combined with diagnostics to identify deficits in cerebral bioenergetics may therefore offer novel therapeutic potential for Alzheimer's disease (AD) prevention and management. Many diet-derived natural bioactive components can govern cellular energy metabolism but their effects on brain aging are not clear. This review examines how nutritional metabolism can regulate brain bioenergetics and mitigate AD risk. We focus on leading mechanisms of cerebral bioenergetic breakdown in the aging brain at the cellular level, as well as the putative causes and consequences of disturbed bioenergetics, particularly at the blood-brain barrier with implications for nutrient brain delivery and nutritional interventions. Novel therapeutic nutrition approaches including diet patterns are provided, integrating studies of the gut microbiome, neuroimaging, and other biomarkers to guide future personalized nutritional interventions.

show abstract

Inflammation, Lifestyle Factors, and the Microbiome‐Gut‐Brain Axis: Relevance to Depression and Antidepressant Action

Donoso

Cryan

Olavarría-Ramírez

et al. 2022

Clin Pharma and Therapeutics

View full text Add to dashboard Cite

Depression is considered a major public health concern, where existing pharmacological treatments are not equally effective across all patients. The pathogenesis of depression involves the interaction of complex biological components, such as the immune system and the microbiota-gut-brain axis. Adjunctive lifestyle-oriented approaches for depression, including physical exercise and special diets are promising therapeutic options when combined with traditional antidepressants. However, the mechanisms of action of these strategies are incompletely understood. Accumulating evidence suggests that physical exercise and specific dietary regimens can modulate both the immune system and gut microbiota composition. Here, we review the current information about the strategies to alleviate depression and their crosstalk with both inflammatory mechanisms and the gut microbiome. We further discuss the role of the microbiota-gut-brain axis as a possible mediator for the adjunctive therapies for depression through inflammatory mechanisms. Finally, we review existing and future adjunctive strategies to manipulate the gut microbiota with potential use for depression, including physical exercise, dietary interventions, prebiotics/probiotics, and fecal microbiota transplantation.

show abstract

Microbiota-derived acetate enables the metabolic fitness of the brain innate immune system during health and disease

Cited by 183 publications

References 68 publications

Flow-cytometry-based protocol to analyze respiratory chain function in mouse microglia

Flow-cytometry-based protocol to analyze respiratory chain function in mouse microglia

Nutritional metabolism and cerebral bioenergetics in Alzheimer's disease and related dementias

Inflammation, Lifestyle Factors, and the Microbiome‐Gut‐Brain Axis: Relevance to Depression and Antidepressant Action

Contact Info

Product

Resources

About