Microbiota adaptation after an alkaline pH perturbation in a full-scale UASB anaerobic reactor treating dairy wastewater

Callejas, Cecilia; Fernandez, A. S. Rubesa; Passeggi, Mauricio; Wenzel, Jorge; Bovio, Patricia; Borzacconi, Liliana; Etchebehere, Claudia

doi:10.1007/s00449-019-02198-3

Cited by 21 publications

(18 citation statements)

References 58 publications

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“…This family belongs to phylum Synergistetes, which was also observed in other reactors treating dairy wastewater (Gunnigle et al, 2015a;Keating et al, 2018;Callejas et al, 2019) and it is known to degrade peptides, proteins and amino acids. On the other hand, contrary to other studies of room/low temperature reactors treating dairy wastewater (Bialek et al, 2011(Bialek et al, , 2014Keating et al, 2018;Callejas et al, 2019), our results showed very little active presence of Firmicutes. Nevertheless, Gunnigle et al (2015a) reported a decreased in Firmicutes associated with low temperature.…”

Section: Discussionmentioning

confidence: 76%

“…Despite the microbial community changes, the pilot reactor performance was always able to recover, with efficient wastewater treatment performance (high methane content, good COD removal). In similar fashion, a full-scale UASB treating dairy wastewater suffered from a pH increase to 9.0 for 2 days (Callejas et al, 2019). The authors observed an effect on reactor performance, as well as a decrease in relative abundance for most phyla, but, also in this case, the reactor and community were able to recover from the pH imbalance.…”

Section: Discussionmentioning

confidence: 78%

“…Nevertheless, Gunnigle et al (2015a) reported a decreased in Firmicutes associated with low temperature. Interestingly, Callejas et al (2019) observed an increase in Firmicutes from 29 to 79% after a pH increase in a full-scale UASB treating dairy wastewater.…”

Section: Discussionmentioning

confidence: 97%

“…On the other hand, the phylum Proteobacteria, commonly found in dairy-treating reactors (Bialek et al, 2011(Bialek et al, , 2014Gunnigle et al, 2015a;Keating et al, 2018;Callejas et al, 2019), aside from Pseudomonas, represented 8 to 16% of the active community, although no family was highly abundant. These values are much lower than the 62% relative abundance of this phylum observed by Gunnigle et al (2015a), but more similar to the 27% observed by Callejas et al (2019). Overall, our results and the literature indicate that a high relative abundance of methanogens, specially Methanotrix species, and bacterial members of Bacteroidetes, Synergistaceae and Proteobacteria are the core players in active communities of AD-digesters treating dairy wastewater at low temperature.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Microbial Community Redundancy and Resilience Underpins High-Rate Anaerobic Treatment of Dairy-Processing Wastewater at Ambient Temperatures

Paulo

Castilla-Archilla

Ramiro-García

et al. 2020

Front. Bioeng. Biotechnol.

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High-rate anaerobic digestion (AD) is a reliable, efficient process to treat wastewaters and is often operated at temperatures exceeding 30 • C, involving energy consumption of biogas in temperate regions, where wastewaters are often discharged at variable temperatures generally below 20 • C. High-rate ambient temperature AD, without temperature control, is an economically attractive alternative that has been proven to be feasible at laboratory-scale. In this study, an ambient temperature pilot scale anaerobic reactor (2 m 3 ) was employed to treat real dairy wastewater in situ at a milk processing plant, at organic loading rates of 1.3 ± 0.6 to 10.6 ± 3.7 kg COD/m 3 /day and hydraulic retention times (HRT) ranging from 36 to 6 h. Consistent high levels of COD removal efficiencies, ranging from 50 to 70% for total COD removal and 70 to 84% for soluble COD removal, were achieved during the trial. Within the reactor biomass, stable active archaeal populations were observed, consisting mainly of Methanothrix (previously Methanosaeta) species, which represented up to 47% of the relative abundant active species in the reactor. The decrease in HRT, combined with increases in the loading rate had a clear effect on shaping the structure and composition of the bacterial fraction of the microbial community, however, without affecting reactor performance. On the other hand, perturbances in influent pH had a strong impact, especially when pH went higher than 8.5, inducing shifts in the microbial community composition and, in some cases, affecting negatively the performance of the reactor in terms of COD removal and biogas methane content. For example, the main pH shock led to a drop in the methane content to 15%, COD removals decreased to 0%, while the archaeal population decreased to ∼11% both at DNA and cDNA levels. Functional redundancy in the microbial community underpinned stable reactor performance and rapid reactor recovery after perturbations.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Microbial Community Redundancy and Resilience Underpins High-Rate Anaerobic Treatment of Dairy-Processing Wastewater at Ambient Temperatures

Paulo

Castilla-Archilla

Ramiro-García

et al. 2020

Front. Bioeng. Biotechnol.

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“…The microbial community in UASB reactors is capable to adapt even to sudden changes in operating parameters that can arise in continuous operations. Bacteria response to alkaline pH perturbations in a full-scale UASB reactor treating dairy wastewater showed that after an accidental pH increase (which affected microbial community structure) the microbial population was able to regain diversity and methanogenic activity [ 58 ]. Granulation fundamentals are not completely understood at the present, despite the number of existing granulation-based treatment plants [ 59 ].…”

Section: Uasb Hydrodynamics and Microbial Communitymentioning

confidence: 99%

Up-Flow Anaerobic Sludge Blanket (UASB) Technology for Energy Recovery: A Review on State-of-the-Art and Recent Technological Advances

2020

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Up-flow anaerobic sludge blanket (UASB) reactor belongs to high-rate systems, able to perform anaerobic reaction at reduced hydraulic retention time, if compared to traditional digesters. In this review, the most recent advances in UASB reactor applications are critically summarized and discussed, with outline on the most critical aspects for further possible future developments. Beside traditional anaerobic treatment of soluble and biodegradable substrates, research is actually focusing on the treatment of refractory and slowly degradable matrices, thanks to an improved understanding of microbial community composition and reactor hydrodynamics, together with utilization of powerful modeling tools. Innovative approaches include the use of UASB reactor for nitrogen removal, as well as for hydrogen and volatile fatty acid production. Co-digestion of complementary substrates available in the same territory is being extensively studied to increase biogas yield and provide smooth continuous operations in a circular economy perspective. Particular importance is being given to decentralized treatment, able to provide electricity and heat to local users with possible integration with other renewable energies. Proper pre-treatment application increases biogas yield, while a successive post-treatment is needed to meet required effluent standards, also from a toxicological perspective. An increased full-scale application of UASB technology is desirable to achieve circular economy and sustainability scopes, with efficient biogas exploitation, fulfilling renewable energy targets and green-house gases emission reduction, in particular in tropical countries, where limited reactor heating is required.

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Sugarcane vinasse extreme thermophilic digestion: a glimpse on biogas free management

Niz

Fuentes

Etchebehere

et al. 2021

Bioprocess Biosyst Eng

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Microbiota adaptation after an alkaline pH perturbation in a full-scale UASB anaerobic reactor treating dairy wastewater

Cited by 21 publications

References 58 publications

Microbial Community Redundancy and Resilience Underpins High-Rate Anaerobic Treatment of Dairy-Processing Wastewater at Ambient Temperatures

Microbial Community Redundancy and Resilience Underpins High-Rate Anaerobic Treatment of Dairy-Processing Wastewater at Ambient Temperatures

Up-Flow Anaerobic Sludge Blanket (UASB) Technology for Energy Recovery: A Review on State-of-the-Art and Recent Technological Advances

Sugarcane vinasse extreme thermophilic digestion: a glimpse on biogas free management

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