Microbiomes of Urine and the Prostate Are Linked to Human Prostate Cancer Risk Groups

Hurst, Rachel; Meader, Emma; Gihawi, Abraham; Rallapalli, Ghanasyam; Clark, Jeremy; Kay, Gemma L.; Webb, Martyn; Manley, Kate; Curley, Helen; Walker, Helen M.; Kumar, Ravi; Schmidt, K.; Crossman, Lisa C.; Eeles, Rosalind A.; Wedge, David C.; Lynch, Andy G.; Massie, Charlie E.; Yazbek-Hanna, Marcelino; Rochester, Mark; Mills, Robert; Mithen, Richard; Τράκα, Μαρία; Ball, Richard Y.; O’Grady, Justin; Brewer, Daniel; Wain, John; Cooper, Christopher S.

doi:10.1016/j.euo.2022.03.006

Cited by 26 publications

(36 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A researcher recently discovered aggressive prostate cancer and its outcome depends on bacteria in the prostate. Genetic analyses of urine and prostate tissue from men with and without prostate cancer determined five species of bacteria causing the more aggressive outcome of prostate cancer [34]. This and other correlations of bacteria to cancer are supportive of the author's theory of the bacteria producing isotopically enriched biomolecules which when absorbed into healthy human and animal tissue cause cancer.…”

Section: Fourth Evidence From Direct Observation Of Bacterial Infecti...mentioning

confidence: 54%

Evidence of Stable Isotope 13C Causing All Cancers

Little

2022

EJPHYSICS

View full text Add to dashboard Cite

Evidence for a general mechanism of genesis of all cancers is given by presenting a puzzle and using recent results of aspartame and acesulfame-K to fit the pieces of the cancer puzzle together for proof of the mechanism of oncogenesis. Aspartame and acesulfame-K have been linked to cancer. Aspartame is a modified dipeptide (methylated phenylalanine (modified) and aspartic acid), so modified amino acids were thought to be more innocuous. The author previously noted isotopically enriched and/or clumped bond-specific amino acids and oligonucleotides have different biochemical reactions for different biology. Acesulfame-K is not a peptide or oligonucleotide but it has some similar chemical structure to oligonucleoside and reversibly decomposes to affect oligonucleosides and affect cells and bacteria cells like E Coli for producing isotopically enriched amino acids indirectly. E. Coli is present in the human digestive system and is known to accelerate stable isotope enrichment of amino acids with 13C, 15N, and/or 17O. Thereby acesulfame-K and aspartame induce isotopic enrichments and excessive 13C, 15N, and/or 17O introductions into proteins and nucleic acids. Such effects of 13C isotopes as induced by the bacteria and these chemicals like aspartame and acesulfame-K may be coupled to effects of static magnetic field(s) and radio frequency electromagnetic waves for explaining the complexity of results and inconsistent results from separating these effects. Thereby the 13C isotope enriched phenylalanine and/or aspartic acid may be the basis of malfunctioning normal cells for the genesis of cancer. Further support for this theory of 13C causing cancer by ingestion of aspartame is given by also noting the mystery and confounding results of monosodium glutamate causing cancer as the glutamate is synthesized by bacterial culture and the resulting ingested glutamic acid enriched with 13C and 15N and possibly 17O causes adverse biochemistry and enzymatics (relative to glutamate from healthy plants and animals rather than the bacterial industrial glutamate in MSG) for cancer and other diseases as explaining the observation of cancer and other diseases from acute and chronic MSG ingestion over months and years. The third case of bacterial production of insulin during the last 25 years for recent correlation to insulin causing cancer relative to less cancer from older methods of insulin production is more evidence of bacterial production of amino acids and peptides causing enriched heavy isotopes and the resulting enriched amino acids and peptides causing cancer relative to ingestion of 13C enriched carbohydrates.

show abstract

Section: Fourth Evidence From Direct Observation Of Bacterial Infecti...mentioning

confidence: 54%

Evidence of Stable Isotope 13C Causing All Cancers

Little

2022

EJPHYSICS

View full text Add to dashboard Cite

show abstract

“…nov . or harei ) in urine sediments strongly correlates with poor PC prognosis determined by the D'Amico score [ 7 ]. Altogether, the available evidence suggests that the local microbiota might affect prostate cancer progression ( Fig.…”

Section: Contribution Of the Local Microbiota To Prostate Carcino-gen...mentioning

confidence: 99%

Effects of the intestinal microbiota on prostate cancer treatment by androgen deprivation therapy

Terrisse¹,

Zitvogel²,

Kroemer³

2022

Microb Cell

View full text Add to dashboard Cite

Prostate cancer (PC) can be kept in check by androgen deprivation therapy (ADT, usually with the androgen synthesis inhibitor abiraterone acetate or the androgen receptor antagonist such as enzalutamide) until the tumor evolves to castration-resistant prostate cancer (CRPC). The transition of hormone-sensitive PC (HSPC) to CPRC has been explained by cancer cell-intrinsic resistance mechanisms. Recent data indicate that this transition is also marked by cancer cell-extrinsic mechanisms such as the failure of ADT-induced PC immunosurveillance, which depends on the presence of immunostimulatory bacteria in the gut. Moreover, intestinal bacteria that degrade drugs used for ADT, as well as bacteria that produce androgens, can interfere with the efficacy of ADT. Thus, specific bacteria in the gut serve as a source of testosterone, which accelerates prostate cancer progression, and men with CRPC exhibit an increased abundance of such bacteria with androgenic functions. In conclusion, the response of PC to ADT is profoundly influenced by the composition of the microbiota with its immunostimulatory, immunosuppressive and directly ADT-subversive elements.

show abstract

“… Used rectal swabs for collection. Urine Hurst et al (2022) 85 46 sequenced samples (24 PC, 22 negative biopsy) with total 318 undergoing urine sediment microscopy 16S rRNA gene amplicon sequencing Porphyromonas , Varibaculum , Peptoniphilus , and Fenollaria spp. newly discovered in PC urine and further associated with poor prognosis (including Fusobacterium ) Limited sample, only 16S and RNA-seq evaluation used for urine.…”

Section: Microbiomes Of Urologic Diseasementioning

confidence: 99%

“…Eisenhofer et al 7 have presented a RIDE checklist to account for cross-contamination and contaminant DNA in blank controls, which includes: a) reporting study design and approaches used to reduce and assess contributions of contamination, b) inclusion of controls to assess contaminant DNA with at least one of each type of negative control, c) determining the level of contamination by comparing biological samples to controls, and d) exploring contaminant taxa and reporting their impact on interpretation. To the best of our knowledge, only two studies in PCa 76 , 85 have stringently adhered to such guidelines identifying that four new bacteria ( Porphyromonas , Varibaculum , Peptoniphilus , and Fenollaria spp.) in patient urine with significant association with metastatic disease.…”

Section: Limitations Of Current Researchmentioning

confidence: 99%