Microbiome-related metabolite changes in gut tissue, cecum content and feces of rats treated with antibiotics

Behr, Christina; Sperber, Saskia; Jiang, Xiaoqi; Strauss, Volker; Kamp, Hennicke; Walk, T.; Herold, Michael; Beekmann, Karsten; Rietjens, I.M.C.M.; Ravenzwaay, Bennard van

doi:10.1016/j.taap.2018.06.028

Cited by 33 publications

(35 citation statements)

References 40 publications

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“…In addition to studies on the rumen, reports on the microbial composition of the feces in dairy calves are abundant. Previous studies have found an association between fecal microbiota and age (Song et al 2018 ), diet (Dill-McFarland et al 2019 ; Wang et al 2019 ), antibiotic therapy (Behr et al 2018 ; Oultram et al 2015 ; Yousif et al 2018 ), and health (Gomez et al 2017 ) in dairy calves. For lactating cows, the core fecal microbiota was identified from ten farms across Northern and Central California, USA (Hagey et al 2019 ).…”

Section: Introductionmentioning

confidence: 98%

Dynamic changes of the fecal bacterial community in dairy cows during early lactation

Huang

Wang

et al. 2020

AMB Expr

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The dynamics of the community structure and composition of the dairy cow fecal bacterial communities during early lactation is unclear, therefore this study was conducted to characterize the fecal bacterial communities in dairy cows during early lactation using 16S rRNA gene sequencing. Feces were sampled from 20 healthy fresh Holstein dairy cows on day 1 (Fresh1d group) and day 14 (Fresh14d group) after calving. After calving, cows were fed the same fresh diet. The dominant phyla Firmicutes and Proteobacteria were decreased (P ≤ 0.01) with lactating progress and phyla Bacteroidetes were increased (P = 0.008) with lactating progress and dietary transition. At family level, the predominant families were Ruminococcaceae (35.23%), Lachnospiraceae (11.46%), Rikenellaceae (10.44%) and Prevotellaceae (6.89%). A total of 14 genera were different between fecal samples from Fresh1d and Fresh14d, included the predominant genera, such as Ruminococcaceae_UCG-005 (P = 0.008), Rikenellaceae_RC9_gut_group (P = 0.043) and Christensenellaceae_R-7_group (P = 0.008). All fecal bacterial communities shared members of the genera Ruminococcaceae_UCG-005, Bacteroides and Rikenellaceae_RC9_gut_group. These findings help to improve our understanding of the composition and structure of the fecal microbial community in fresh cows and may provide insight into bacterial adaptation time and dietary in lactating cows.

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Section: Introductionmentioning

confidence: 98%

Dynamic changes of the fecal bacterial community in dairy cows during early lactation

Huang

Wang

et al. 2020

AMB Expr

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“…This may be due to the dynamic nature of microbial and metabolite interactions, or because samples were not collected at a time where sleep was altered in SF animals. There is conflicting evidence about whether changes in fecal levels of SCFAs, and possibly other bacterial metabolites, correlate with changes in cecal and plasma SCFA concentrations ( 9 , 42 ), and plasma levels of SCFAs may be a better measure of metabolite effects on blood pressure ( 31 ). Including both systemic and fecal metabolite measures can be considered in future work to further understand relationships between the gut microbiome/metabolome and blood pressure.…”

Section: Discussionmentioning

confidence: 99%

Sleep fragmentation increases blood pressure and is associated with alterations in the gut microbiome and fecal metabolome in rats

Maki

Burke

Calik

et al. 2020

Physiological Genomics

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The gut microbiota, via the production of metabolites entering the circulation, plays a role in blood pressure regulation. Blood pressure is also affected by the characteristics of sleep. To date, no studies have examined relationships among the gut microbiota/metabolites, blood pressure, and sleep. We hypothesized that fragmented sleep is associated with elevated mean arterial pressure, an altered and dysbiotic gut microbial community, and changes in fecal metabolites. In our model system, rats were randomized to 8 h of sleep fragmentation during the rest phase (light phase) or were undisturbed (controls) for 28 consecutive days. Rats underwent sleep and blood pressure recordings, and fecal samples were analyzed during: baseline ( days −4 to −1), early sleep fragmentation ( days 0–3), midsleep fragmentation ( days 6–13), late sleep fragmentation ( days 20–27), and recovery/rest ( days 28–34). Less sleep per hour during the sleep fragmentation period was associated with increased mean arterial pressure. Analyses of gut microbial communities and metabolites revealed that putative short chain fatty acid-producing bacteria were differentially abundant between control and intervention animals during mid-/late sleep fragmentation and recovery. Midsleep fragmentation was also characterized by lower alpha diversity, lower Firmicutes:Bacteroidetes ratio, and higher Proteobacteria in intervention rats. Elevated putative succinate-producing bacteria and acetate-producing bacteria were associated with lower and higher mean arterial pressure, respectively, and untargeted metabolomics analysis demonstrates that certain fecal metabolites are significantly correlated with blood pressure. These data reveal associations between sleep fragmentation, mean arterial pressure, and the gut microbiome/fecal metabolome and provide insight to links between disrupted sleep and cardiovascular pathology.

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“…[ 34,35 ] Moreover, use of anaerobic faecal in vitro incubations to study colonic metabolism was previously evaluated in a study in which microbial‐related in vivo metabolic changes in gut tissue, cecum content, and feces of rats treated with antibiotics were compared. [ 36 ] Based on the results obtained it was concluded that “as a non‐invasive sampling method, feces provide a suitable matrix for studies on metabolism by the gut microbiota.” [ 36 ]…”

Section: Discussionmentioning

confidence: 99%

Interindividual Differences in Human In Vitro Intestinal Microbial Conversion of Green Tea (‐)‐Epigallocatechin‐3‐O‐Gallate and Consequences for Activation of Nrf2 Mediated Gene Expression

Liu

Vervoort

Elzen

et al. 2020

Molecular Nutrition Food Res

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Scope An in vitro faecal incubation model combined with reporter gene assay based testing strategy is developed to characterize interindividual differences in the gut microbial conversion of (‐)‐epigallocatechin‐3‐O‐gallate (EGCG) and its consequences for potential activation of Nrf2‐mediated gene expression. Method & Results Anaerobic human faecal incubations are performed to characterize the microbial metabolism of EGCG including interindividual variability. EGCG derived intestinal microbial metabolite patterns show substantial interindividual differences that are correlated to relative microbial abundances determined by 16S rRNA sequencing. Results obtained show the time‐dependent formation of gallic acid, pyrogallol, phenylpropane‐2‐ols, phenyl‐γ‐valerolactones, and 5‐(3′,5′‐dihydroxyphenyl)valeric acid as the major metabolites, with substantial interindividual differences. The activity of the formed metabolites in the activation of EpRE‐mediated gene expression is tested by EpRE‐LUX reporter gene assay. In contrast to EGCG, at low micromolar concentrations, especially gallic acid, pyrogallol, and catechol induce significant activity in the EpRE‐LUX assay. Conclusions Given these results and taking the level of formation into account, it is concluded that especially gallic acid and pyrogallol contribute to the EpRE‐mediated beneficial effects of EGCG. The interindividual differences in the formation may result in interindividual differences in the beneficial effects of EGCG and green tea consumption.

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Microbiome-related metabolite changes in gut tissue, cecum content and feces of rats treated with antibiotics

Cited by 33 publications

References 40 publications

Dynamic changes of the fecal bacterial community in dairy cows during early lactation

Dynamic changes of the fecal bacterial community in dairy cows during early lactation

Sleep fragmentation increases blood pressure and is associated with alterations in the gut microbiome and fecal metabolome in rats

Interindividual Differences in Human In Vitro Intestinal Microbial Conversion of Green Tea (‐)‐Epigallocatechin‐3‐O‐Gallate and Consequences for Activation of Nrf2 Mediated Gene Expression

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