Microbiome of the upper nasal cavity of beef calves prior to weaning12

McDaneld, T. G.; Kuehn, L. A.; Keele, J. W.

doi:10.1093/jas/skz119

Cited by 9 publications

(6 citation statements)

References 20 publications

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Section: Discussionmentioning

confidence: 99%

“…Bovine respiratory disease is one of the most common causes of health and economic losses in the feedlot and closely linked to an animal’s commensal bacteria populations in the respiratory tract including Mycoplasma [ 68 , 69 ]. Several Mycoplasma species are opportunistic pathogens present in symptomatic and asymptomatic cattle with Mycoplasma bovis being the most pathogenic [ 47 , 55 , 69 ]. A recent study reported a shared metacommunity between the lung and nasopharynx for Mycoplasma species and suggested the nasopharynx as the best target for management strategies against BRD [ 61 ], as it could prevent for example Mycoplasma adherence.…”

Section: Discussionmentioning

confidence: 99%

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Feeding selenium-biofortified alfalfa hay during the preconditioning period improves growth, carcass weight, and nasal microbial diversity of beef calves

et al. 2020

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We previously reported that feeding Se-biofortified alfalfa hay to weaned beef calves in a preconditioning program decreases morbidity and mortality during the feedlot period. To understand the mode of action by which supranutritional Se supplementation supports calf health, we examined the effect of agronomic Se-biofortification on nasal microbiome and fecal parasites. Recently weaned Angus-cross beef calves (n = 30) were randomly assigned to two groups and fed an alfalfa hay-based diet for 9 weeks in a preconditioning program. Alfalfa hay was harvested from fields fertilized with sodium selenate at a rate of 0 or 90 g Se/ha. Calculated Se intake from dietary sources was 1.09 and 27.45 mg Se/calf per day for calves consuming alfalfa hay with Se concentrations of 0.06 and 3.47 mg Se/kg dry matter, respectively. Feeding Se-biofortified alfalfa hay for 9 weeks was effective at increasing whole-blood Se concentrations (556 ± 11 vs 140 ± 11 ng/mL; P < 0.001) and increasing body weight (PTreatment, = 0.03) in weaned beef calves. Slaughter yield grades were higher for calves that had been fed Se-enriched alfalfa hay during the preconditioning period (PTreatment = 0.008). No significant differences were observed in fecal parasite load, which remained low. The nasal microbiome and microbiota diversity within calves and across calves expanded from weaning (week 0) to the feedlot period (week 12), which was promoted by feeding Se-biofortified alfalfa hay. Especially concerning was the expansion of nasal Mycoplasmataceae in the feedlot, which reached over 50% of the total microbiota in some calves. In conclusion, we identified dietary Se-biofortified alfalfa hay as a potential promoter of nasal microbiome genome and microbiota diversity, which may explain in part high-Se benefits for prevention of bovine respiratory disease complex in beef calves.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Feeding selenium-biofortified alfalfa hay during the preconditioning period improves growth, carcass weight, and nasal microbial diversity of beef calves

et al. 2020

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“…The supernatants were stored in microtubes at − 80°C until analysis. All laboratory analyses were performed within 24 h. Nasal swabs were taken as Tara G. McDaneld described in his study [42]. Nasal swabs were collected from the upper nasal cavity of all donkeys using 15 cm nasal swabs.…”

Section: Sample Collectionmentioning

confidence: 99%

Effects of long-distance transportation on blood constituents and composition of the nasal microbiota in healthy donkeys

Zhao

Jiang

Ji³

et al. 2020

BMC Vet Res

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Background This study aims to determine the effects of transportation on the nasal microbiota of healthy donkeys using 16S rRNA sequencing. Results Deep nasal swabs and blood were sampled from 14 donkeys before and after 21 hours’ long-distance transportation. The values of the plasma hormone (cortisol (Cor), adrenocorticotrophic hormone (ACTH)), biochemical indicators (total protein (TP), albumin (ALB), creatinine (CREA), lactic dehydrogenase (LDH), aspartate transaminase (AST), creatine kinase (CK), blood urea (UREA), plasma glucose (GLU)) and blood routine indices (white blood cell (WBC), lymphocyte (LYM), neutrophil (NEU), red blood cell (RBC), hemoglobin (HGB)) were measured. 16S rRNA sequencing was used to assess the nasal microbiota, including alpha diversity, beta diversity, and phylogenetic structures. Results showed that levels of Cor, ACTH, and heat-shock protein 90 (HSP90) were significantly increased (p < 0.05) after long-distance transportation. Several biochemical indicators (AST, CK) and blood routine indices (Neu, RBC, and HGB) increased markedly (p < 0.05), but the LYM decreased significantly (p < 0.05). Nine families and eight genera had a mean relative abundance over 1%. The predominant phyla in nasal microbiota after and before transportation were Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Transportation stress induced significant changes in terms of nasal microbiota structure compared with those before transportation based on principal coordinate analysis (PCoA) coupled with analysis of similarities (ANOSIM) (p < 0.05). Among these changes, a notably gain in Proteobacteria and loss in Firmicutes at the phylum level was observed. Conclusions These results suggest transportation can cause stress to donkeys and change the richness and diversity of nasal microbiota. Further studies are required to understand the potential effect of these microbiota changes on the development of donkey respiratory diseases.

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“…The most common phyla overall in the nares of calves prior to weaning and consequently not exposed to other calves included Proteobacteria, Bacteroidetes, and Firmicutes, while the most common genera were Moraxella , Mannheimia , and Promicromonospora. Streptococcus , Bacteroides , and Clostridium were present as well [ 194 ]. Increased Moraxella and Streptococcus were also noted in unhealthy cattle compared to healthy cattle.…”

Section: Alternative Animal Modelsmentioning

confidence: 99%

Factors Limiting the Translatability of Rodent Model–Based Intranasal Vaccine Research to Humans

Cai

Cui

2022

AAPS PharmSciTech

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The intranasal route of vaccination presents an attractive alternative to parenteral routes and offers numerous advantages, such as the induction of both mucosal and systemic immunity, needle-free delivery, and increased patient compliance. Despite demonstrating promising results in preclinical studies, however, few intranasal vaccine candidates progress beyond early clinical trials. This discrepancy likely stems in part from the limited predictive value of rodent models, which are used frequently in intranasal vaccine research. In this review, we explored the factors that limit the translatability of rodent-based intranasal vaccine research to humans, focusing on the differences in anatomy, immunology, and disease pathology between rodents and humans. We also discussed approaches that minimize these differences and examined alternative animal models that would produce more clinically relevant research.

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Microbiome of the upper nasal cavity of beef calves prior to weaning12

Abstract: Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). 2 USDA is an equal opportunity provider and employer.

Cited by 9 publications

References 20 publications

Feeding selenium-biofortified alfalfa hay during the preconditioning period improves growth, carcass weight, and nasal microbial diversity of beef calves

Feeding selenium-biofortified alfalfa hay during the preconditioning period improves growth, carcass weight, and nasal microbial diversity of beef calves

Effects of long-distance transportation on blood constituents and composition of the nasal microbiota in healthy donkeys

Factors Limiting the Translatability of Rodent Model–Based Intranasal Vaccine Research to Humans

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