Microbiome-Independent Effects of Antibiotics in a Murine Model of Nosocomial Infections

Abstract

Antimicrobial-resistant (AMR) pathogens are responsible for over 2.8 million infections and over 35,000 deaths per year in the United States. To study these microbes, animal models that are susceptible to these pathogens are required.

The tempo and mode of gene regulatory programs during bacterial infection

“…Cells equivalent to state 5 were selected based on their expression of TNF ( Figure S5F ) and the ratio of modules between them was measured revealing greater representation of later modules in the WT compared with the ‘‘toxin-less’’ USA300 strain ( Figure 5H ). We also infected cells with seven other strains ( Table S2 ; Lacey et al, 2022 ; Rose et al, 2015 ) to control for the effect of these toxins rather than other strain-specific differences and found no significant difference in trajectory analysis, suggesting that this shift in trajectory was indeed toxin-mediated ( Table S2 and Figures S5G – S5I ).…”