Microbiology of the Burn Compromised Patient

Holder, Ian Alan

doi:10.1007/978-1-4471-3454-1_11

Cited by 7 publications

(3 citation statements)

References 41 publications

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“…The human skin is normally colonized by a great variety of bacterial species, such as staphylococcal, micrococcal, and Corynebacterium species which form the indigenous microbiota and an efficient barrier to microbial invaders. Whenever this barrier is broken, e.g., by trauma, surgery or burn, microbes from the patient's skin flora or the environment colonize and may induce an infection (5,10,31). The most common wound pathogen, besides group A streptococci, is Staphylococcus aureus.…”

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confidence: 99%

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Growth Conditions Influence Expression of Cell Surface Hydrophobicity of Staphylococci and Other Wound Infection Pathogens

Ljungh

Wadström

1995

Microbiology and Immunology

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The initial adhesion of microbes to tissue and solid surfaces can be mediated by hydrophobic interaction. Expression of microbial cell surface hydrophobicity (CSH) is influenced by growth conditions, and often best expressed after growth under nutrient-poor conditions, or "starvation." In the present study, the CSH of 133 strains of Enterobacteriaceae, Staphylococcus aureus, coagulase-negative staphylococci, Enterococcus faecalis, group A streptococcus, Pseudomonas aeruginosa, Clostridium perfringens, Bacteroidesfragilis, Peptococcus magnus, and of 8 Candida albicans strains was measured by the salt aggregation test after growth on hematin agar in a 5% CO2 atmosphere, or under anaerobiosis. Cells of all but 8 strains expressed pronounced or moderate CSH, i.e., they aggregated in 0.01-2 M ammonium sulfate. When the agar surface was covered by human serum (diluted 1:5) to mimic growth conditions in a wound, 94 strains expressed higher CSH, and 44 strains the same CSH as after growth without serum. The CSH of 12 strains of different species was measured after growth on blood, hematin and PDM agar, with or without serum, and in an aerobic or a 5% CO2 atmosphere. The highest CSH was expressed after growth in 5% CO2 with serum, and the lowest growth after on blood agar in aerobic atmosphere. Identical results were obtained with native and heat-inactivated (56 C, 20 min) serum. The reduced surface tension obtained in 5% CO2, as well as yet unidentified serum factors, promotes expression of CSH.

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“…The most common wound pathogen, besides group A streptococci, is Staphylococcus aureus. Pseudomonas aeruginosa and the fungal pathogen Candida albicans are of particular importance in burn wounds (10). In abdominal wounds gram-negative enteric bacteria are frequently isolated along with anaerobic bacteria from the indigenous intestinal flora.…”

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Growth Conditions Influence Expression of Cell Surface Hydrophobicity of Staphylococci and Other Wound Infection Pathogens

Ljungh

Wadström

1995

Microbiology and Immunology

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“…Displaying increased resistance to antimicrobial agents, P. aeruginosa burn infections are particularly severe and have for long been of great concern. A previous study mentioned that P. aeruginosa caused more fatal septicaemia secondary to burn infections than the combined deaths from all other Gram negative bacteria (Holder et al 1990). Another study also reported that P. aeruginosa remains a serious cause of infection and septic mortality in burn patients, particularly when nosocomially acquired (Tredget et al 2004).…”

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confidence: 98%

In Vivo Evaluation of a Recombinant N-acylhomoserine Lactonase Formulated in a Hydrogel Using a Murine Model Infected With Mdr Pseudomonas Aeruginosa Clinical Isolate, CCASUP2

Sakr

Elkhatib

Aboshanab

et al. 2021

Preprint

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Failure in the treatment of P. aeruginosa, due to its broad spectrum of resistance, has been associated with increased patient mortality. One alternative approach for infection control is quorum quenching which was found to decrease virulence of such pathogen. In this study, the efficiency of a recombinant Ahl-1 lactonase formulated as a hydrogel was investigated to control the infection of multidrug resistant (MDR) P. aeruginosa infected burn using a murine model. The recombinant N-acylhomoserine lactonase (Ahl-1) was formulated as a hydrogel. To test its ability to control the infection of MDR P. aeruginosa, a thermal injury model was used. Survival rate, and systemic spread of the infection were evaluated. Histopathological examination of the animal dorsal skin was also done for monitoring the healing and cellular changes at the site of infection. Survival rate in the treated group was 100% relative to 40% in the control group. A decrease of up to 3 logs of bacterial count in the blood samples of the treated animals relative to the control group and a decrease of up to 4 logs and 2.3 logs of bacteria in lung and liver samples, respectively were observed. Histopathological examination revealed more enhanced healing process in the treated group. Accordingly, by promoting healing of infected MDR P. aeruginosa burn and by reducing systemic spread of the infection as well as decreasing mortality rate, Ahl-1 hydrogel application is a promising strategy that can be used to combat and control P. aeruginosa burn infections.

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P. aeruginosa Burn Infections: Pathogenesis and Treatment

Holder¹

1993

Pseudomonas Aeruginosa as an Opportunistic Pathogen

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Microbiology of the Burn Compromised Patient

Cited by 7 publications

References 41 publications

Growth Conditions Influence Expression of Cell Surface Hydrophobicity of Staphylococci and Other Wound Infection Pathogens

Growth Conditions Influence Expression of Cell Surface Hydrophobicity of Staphylococci and Other Wound Infection Pathogens

In Vivo Evaluation of a Recombinant N-acylhomoserine Lactonase Formulated in a Hydrogel Using a Murine Model Infected With Mdr Pseudomonas Aeruginosa Clinical Isolate, CCASUP2

P. aeruginosa Burn Infections: Pathogenesis and Treatment

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